Effects of CLA and ALA on Genes and Protein Expressions of Nuclear Transcription Factor Nrf2 and GSTA1 in HepG2 Cell

LI Guangliang, ZHANG Xiuying, QI Yue, HAO Lihong, ZHANG Jinming

doi:10.3969/j.issn.1006-267x.2015.04.021

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 4: 1172 - 1177

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.04.021

Molecular Nutrition

Effects of CLA and ALA on Genes and Protein Expressions of Nuclear Transcription Factor Nrf2 and GSTA1 in HepG2 Cell

Expand

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

Received date: 2014-11-21

Online published: 2015-04-21

Fold

Abstract

The purpose of the study was to explore the effects of conjugated linoleic acid (CLA)and α-linolenic acid (ALA)on mRNA and protein expression of nuclear transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) and glutathione S-transferase A1 (GSTA1) in HepG2 cells. The experiment groups were used 0.2, 0.5 and 1.0 mmol/L CLA and ALA treated HepG2 cells for 24 h, the mRNA expression levels were quantified by quantitative reverse transcription PCR analysis, and the protein expression levels were detected by western blotting method. The results showed as follows: compared with the control group, the mRNA and protein expressions of Nrf2 and GSTA1 were significantly incresed in cells treated with CLA with the concentration of 0.2 and 0.5 mmol/L (P<0.01), and with the CLA concentration increased, the mRNA and protein expressions of Nrf2 and GSTA1 were decreased. However, when HepG2 cells were treated with ALA, the mRNA and protein expressions of Nrf2 and GSTA1 were significantly increased with the ALA concentration increased of compared to control group (P<0.01). In conclusion, CLA with a relatively lower concentration elevates the mRNA and protein expressions of Nrf2 and GSTA1, on the contrary, the mRNA and protein expressions of Nrf2 and GSTA1 are increase gradually with the CLA concentration increased.

Key words： conjugated linoleic acid; α-linolenic acid; HepG 2 cell; nuclear factor erythroid 2-related factor 2; glutathione S-transferase A1

Cite this article

LI Guangliang, ZHANG Xiuying, QI Yue, HAO Lihong, ZHANG Jinming . Effects of CLA and ALA on Genes and Protein Expressions of Nuclear Transcription Factor Nrf2 and GSTA1 in HepG2 Cell[J]. Chinese Journal of Animal Nutrition, 2015 , 27(4) : 1172 -1177 . DOI: 10.3969/j.issn.1006-267x.2015.04.021

References

[1] MONTEIRO J, LESLIE M, MOGHADASIAN M H, et al.The role of n-6 and n-3 polyunsaturated fatty acids in the manifestation of the metabolic syndrome in cardiovascular disease and non-alcoholic fatty liver disease[J].Food & Function, 2014, 5(3):426-435.

[2] PATTERSON E, WALL R, FITZGERALD G F, et al.Health implications of high dietary omega-6 polyunsaturated fatty acids[J].Journal of Nutrition and Metabolism, 2012, 2012:539426.

[3] POUDYAL H, PANCHAL S K, DIWAN V, et al.Omega-3 fatty acids and metabolic syndrome:effects and emerging mechanisms of action[J].Progress in Lipid Research, 2011, 50(4):372-387.

[4] BATAILLE A M, MANAUTOU J E.Nrf2:a potential target for new therapeutics in liver disease[J].Clinical Pharmacology & Therapeutics, 2012, 92(3):340-348.

[5] WAKABAYASHI N, SLOCUM S L, SKOKO J J, et al.When NRF2 talks, who's listening?[J].Antioxidants & Redox Signaling, 2010, 13(11):1649-1663.

[6] LIANG F Q, ALSSADI R, MOREHEAD P, et al.Enhanced expression of glutathione-S-transferase A1-1 protects against oxidative stress in human retinal pigment epithelial cells[J].Experimental Eye Research, 2005, 80(1):113-119.

[7] SYKIOTIS G P, HABEOS I G, SAMUELSON A V, et al.The role of the antioxidant and longevity-promoting Nrf2 pathway in metabolic regulation[J].Current Opinion in Clinical Nutrition and Metabolic Care, 2011, 14(1):41-48.

[8] QI X L, WU S G, ZHANG H J, et al.Effects of dietary conjugated linoleic acids on lipid metabolism and antioxidant capacity in laying hens[J].Archives of Animal Nutrition, 2011, 65(5):354-365.

[9] MOLLICA M P, TRINCHESE G, CAVALIERE G, et al.c9, t11-conjugated linoleic acid ameliorates steatosis by modulating mitochondrial uncoupling and Nrf2 pathway[J].Journal of Lipid Research, 2014, 55(5):837-849.

[10] SIMOPOULOS A P.The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases[J].Experimental Biology and Medicine, 2008, 233(6):674-688.

[11] MIRMIRAN P, HOSSEINPOUR-NIAZI S, NADERI Z, et al.Association between interaction and ratio of ω-3 and ω-6 polyunsaturated fatty acid and the metabolic syndrome in adults[J].Nutrition, 2012, 28(9):856-863.

[12] BEYAZIT Y, PURNAK T, KEKILLI M.Omega-3 fatty acids:a promising alternative treatment approach for patients with nonalcoholic fatty liver disease[J].Digestive Diseases and Sciences, 2010, 55(8):2422-2423.

[13] KOHJIMA M, ENJOJI M, HIGUCHI N, et al.The effects of unsaturated fatty acids on lipid metabolism in HepG2 cells[J].In Vitro Cellular & Developmental Biology-Animal, 2009, 45(1/2):6-9.

[14] HUERTA-OLVERA S G, MACÍAS-BARRAGÁN J, RAMOS-MÁRQUEZ M E, et al.Alpha-lipoic acid regulates heme oxygenase gene expression and nuclear Nrf2 activation as a mechanism of protection against arsenic exposure in HepG2 cells[J].Environmental Toxicology and Pharmacology, 2010, 29(2):144-149.

[15] DELPLANQUE B, DU Q, AGNANI G, et al.A dairy fat matrix providing alpha-linolenic acid (ALA) is better than a vegetable fat mixture to increase brain DHA accretion in young rats[J].Prostaglandins, Leukotrienes and Essential Fatty Acids, 2013, 88(1):115-120.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References