Short Communications

Arginine Regulates Growth-Related Factor Gene Expression through Endocrine Pathway of Piglets under Oxidative Stress

Expand
  • Animal Disease-Resistance Nutrition Key Laboratory of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China

Received date: 2014-11-21

  Online published: 2015-04-21

Abstract

The present study was conducted to test the effects of arginine (Arg) supplementation on endocrine and growth-relative factor gene expression of piglets under oxidative stress, in order to explore the mechanism of Arg on anti-oxidative stress. A total of 24 PIC weaned female piglets were randomly allocated into 4 groups with 6 replicates per group, and each replicate had 1 piglet. Piglets in those groups were fed basal diet+injected sterile saline (control group), basal diet+injected Diquat (oxidative stress group), basal diet supplemented with 0.8% Arg+injected Diquat (0.8% Arg group), and basal diet supplemented with 1.6% Arg+injected Diquat (1.6% Arg group), respectively. On day 8 of experiment, piglets were injected intraperitoneally either with diquat (experiment groups) or sterile saline (control group) at 10 mg/kg BW to induce oxidative stress. The experiment lasted for 11 d. The results showed as follows: oxidative stress significantly decreased the concentrations of insulin and insulin-like growth factor-Ⅰ (IGF-Ⅰ) in plasma at 24 h post-stress of piglets (P < 0.05). Supplementation of 1.6% Arg significantly increased the concentration of IGF-Ⅰ in plasma at 48 and 96 h post-stress of piglets under oxidative stress (P < 0.05). 2) Oxidative stress didnot affect the activities of nitric oxide synthases (NOS) in plasma of piglets (P>0.05). However, Oxidative stress significantly decreased the activities of NOS in liver of piglets (P < 0.05). Supplementation of 1.6 % Arg significantly increased the activities of NOS in liver of piglets under oxidative stress (P < 0.05). 3) Oxidative stress significantly decreased IGF-Ⅰ, insulin-like growth factor-Ⅰ receptor (IGF-ⅠR) and insulin-like growth factor-binding protein 3 (IGFBP3) mRNA expression levels in liver of piglets (P < 0.05), but had no significant effects on the mRNA expression levels of those 3 genes in muscle (P>0.05). Supplementation of 1.6% Arg significantly increased the mRNA expression levels of IGF-Ⅰ, IGFBP3 mRNA in liver, and IGF-Ⅰ, IGF-ⅠR and IGFBP3 in muscle of piglets under oxidative stress (P < 0.05). It is concluded that Arg can up-regulate growth-related factor gene expression through stimulating the secretion of insulin and IGF-Ⅰ, which might be one of the ways of Arg to alleviate oxidative stress in piglets.

Cite this article

ZHENG Ping, YU Bing, HE Jun, YU Jie, MAO Xiangbing, LUO Junqiu, HUANG Zhiqing, WANG Quyuan, CHEN Daiwen . Arginine Regulates Growth-Related Factor Gene Expression through Endocrine Pathway of Piglets under Oxidative Stress[J]. Chinese Journal of Animal Nutrition, 2015 , 27(4) : 1214 -1221 . DOI: 10.3969/j.issn.1006-267x.2015.04.027

References

S H.Nitric oxide:a physiological messenger molecule[J].Annual Review of Biochemistry, 1994, 63:175-195.

[5] WU G Y, BAZER F W, DAVIS T A, et al.Important roles for the arginine family of amino acids in swine nutrition and production[J].Livestock Science, 2007, 112(1/2):8-22.

[6] SIASOS G, TOUSOULIS D, ANTONIADES C, et al.L-arginine, the substrate for no synthesis:an alternative treatment for premature atherosclerosis?[J].International Journal of Cardiology, 2007, 116(3):300-308.  

[7] BARRY D P, ASIM M, LEWIS N D, et al.Increased L-arginine availability enhances Helicobacter pylori colonization in the chronic mouse model[J].Gastroenterology, 2009, 136(5):A-104.

[8] HERNANDEZ A, HANSEN C F, MULLAN B P, et al.L-arginine supplementation of milk liquid or dry diets fed to pigs after weaning has a positive effect on production in the first three weeks after weaning at 21 days of age[J].Animal Feed Science and Technology, 2009, 154(1/2):102-111.

[9] BERARD J, BEE G.Effects of dietary L-arginine supplementation to gilts during early gestation on foetal survival, growth and myofiber formation[J].Animal, 2010, 4(10):1680-1687.  

[10] KONG X F, TAN B, YIN Y L, et al.L-arginine stimulates the mTOR signaling pathway and protein synthesis in porcine trophectoderm cells[J].The Journal of Nutritional Biochemistry, 2012, 23(9):1178-1183.  

[11] 吴琛, 刘俊锋, 孔祥峰, 等.日粮添加精氨酸对环江香猪肉质及抗氧化功能的影响[C]//姚军虎.第六次全国饲料营养学术研讨会论文集.北京:中国畜牧兽医学会动物营养学分会, 2010.

[12] ZHENG P, YU B, HE J, et al.Protective effects of dietary arginine supplementation against oxidative stress in weaned piglets[J].British Journal of Nutrition, 2013, 109(12):2253-2260.  

[13] RYTWO G, TROPP D, SERBAN C.Adsorption of diquat, paraquat and methyl green on sepiolite:experimental results and model calculations[J].Applied Clay Science, 2002, 20(6):273-282.  

[14] ROGERS L K, BATES C M, WELTY S E, et al.Diquat induces renal proximal tubule injury in glutathione reductase-deficient mice[J].Toxicology and Applied Pharmacology, 2006, 217(3):289-298.  

[15] BENZICK A E, REDDY S L, GUPTA S, et al.Diquat- and acetaminophen-induced alterations of biliary efflux of iron in rats[J].Biochemical Pharmacology, 1994, 47(11):2079-2085.  

[16] BLAKEMAN D P, RYAN T P, JOLLY R A, et al.Diquat-dependent protein carbonyl formation:identification of lipid-dependent and lipid-independent pathways[J].Biochemical Pharmacology, 1995, 50(7):929-935.  

[17] SPALDING D J M, MITCHELL J R, JAESCHKE H, et al.Diquat hepatotoxicity in the fischer-344 rat:the role of covalent binding to tissue proteins and lipids[J].Toxicology and Applied Pharmacology, 1989, 101(2):319-327.  

[18] NRC.Nutrient requirements of swine[S].10th ed.Washington, D.C.:National Academy Press, 1998.

[19] PFAFFL M W.A new mathematical model for relative quantification in real-time RT-PCR[J].Nucleic Acids Research, 2001, 29(9):e45.

[20] SUSCHEK C V, SCHNORR O, HEMMRICH K, et al.Critical role of L-arginine in endothelial cell survival during oxidative stress[J].Circulation, 2003, 107(20):2607-2614.

[21] WRIGHT K J, BALAY R, HILL C M, et al.Integrated adrenal, somatotropic, and immune responses of growing pigs to treatment with lipopolysaccharide[J].Journal of Animal Science, 2000, 78(7):1892-1899.

[22] SALERI R, BARATTA M, MAINARDI G L, et al.IGF-1, IGFBP-2 and -3 but not GH concentration are different in normal and poor growing piglets[J].Reproduction Nutrition Development, 2001, 41(2):163-172.  

[23] PRIEGO T, DE CÁCERES I I, MARTIN A I, et al.No plays a role in LPS-induced decreases in circulating IGF-Ⅰ and IGFBP-3 and their gene expression in the liver[J].American Journal of Physiology:Endocrinology and Metabolism, 2004, 286(1):E50-E56.

[24] FRANK J W, ESCOBAR J, NGUYEN H V, et al.Oral N-carbamylglutamate supplementation increases protein synthesis in skeletal muscle of piglets[J].The Journal of Nutrition, 2007, 137(2):315-319.

[25] WU G, MEININGER C J, KNABE D A, et al.Arginine nutrition in development, health and disease[J].Current Opinion in Clinical Nutrition and Metabolic Care, 2000, 3(1):59-66.  

[26] LIU Y L, HUANG J J, HOU Y Q, et al.Dietary arginine supplementation alleviates intestinal mucosal disruption induced by Escherichia coli lipopolysaccharide in weaned pigs[J].British Journal of Nutrition, 2008, 100(3):552-560.  

[27] JALLALI N, RIDHA H, THRASIVOULOU C, et al.Modulation of intracellular reactive oxygen species level in chondrocytes by IGF-1, FGF, and TGF-β1[J].Connective Tissue Research, 2007, 48(3):149-158.  

[28] PI Y, GOLDENTHAL M J, MARIN-GARCIA J.Mitochondrial involvement in IGF-1 induced protection of cardiomyocytes against hypoxia/reoxygenation injury[J].Molecular and Cellular Biochemistry, 2007, 301(1/2):181-189.

[29] BAREGAMIAN N, SONG J, JESCHKE M G, et al.IGF-1 protects intestinal epithelial cells from oxidative stress-induced apoptosis[J].Journal of Surgical Research, 2006, 136(1):31-37.  

[30] ZHAO J P, JIAO H C, SONG Z G, et al.Effects of L-arginine supplementation on glucose and nitric oxide (no) levels and activity of no synthase in corticosterone-challenged broiler chickens (Gallus gallus)[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 2009, 150(4):474-480.  

[31] DELAFONTAINE P, SONG Y H, LI Y.Expression, regulation, and function of IGF-1, IGF-1R, and IGF-1 binding proteins in blood vessels[J].Arteriosclerosis Thrombosis and Vascular Biology, 2004, 24(3):435-444.  
Outlines

/