Effects of Dietary Physically Effective Neutral Detergent Fiber Level on Rumen Fermentation Parameters and Cellulolytic Bacteria of 8 to 10-Month-Old Heifers

CHEN Qing, WANG Hongrong, GE Rufang, CUI Huihui, WANG Mengzhi, YU Lihuai, LIN Miao

doi:10.3969/j.issn.1006-267x.2015.04.031

2015 , Vol. 27 >Issue 4: 1243 - 1251

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.04.031

Short Communications

Effects of Dietary Physically Effective Neutral Detergent Fiber Level on Rumen Fermentation Parameters and Cellulolytic Bacteria of 8 to 10-Month-Old Heifers

Expand

College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2014-11-21

Online published: 2015-04-21

Fold

Abstract

The objective of this experiment was to evaluate effects of dietary physically effective neutral detergent fiber (peNDF) level on rumen fermentation parameters and cellulolytic bacteria of 8 to 10-month-old heifers. Twenty-four Chinese Holstein heifers with similar month of age and body weight were randomly divided into 4 groups. Heifers were offered one of four diets, which were chemically identical but included different peNDF levels [22.73%, 23.92%, 25.85% and 28.39%] through cutting the roughage of Chinese wildrye into different lengths. The pre-test lasted for 10 days, and the test lasted for 60 days. The results showed as follows: 1) the intakes of dry matter, neutral detergent fiber and acid detergent fiber of heifers were not significantly affected by peNDF level (P > 0.05). 2) Rumen fluid pH increased with the increasing peNDF level, 22.73% group was significantly lower than the other 3 groups (P < 0.01); there were no effects of peNDF level on total volatile fatty acid concentration, and acetate and butyrate contents (P > 0.05), however, with the increasing of peNDF level, propionate content decreased, and 22.73% group was significantly higher than 23.92% group (P < 0.01) and 28.39% group (P < 0.05); the ratio of acetate to propionate of 23.92% group was significantly higher than that of 22.73% group (P < 0.01) and 25.85% group(P < 0.05); ammonia-N concentration of 25.85% group was significantly higher than that of the other 3 groups (P < 0.01 or P < 0.01). 3) Using 22.73% group as control, the relative content of Fibrobacter succinogene of the other 3 groups was increased with increasing peNDF level, and that of 28.39% group was the highest, but was still significantly lower than that of 22.73% group (P < 0.01); with the increasing of peNDF level, the relative content of Ruminococcus flave faciensk, Ruminococcus Albus and Prevotella rumicola increased initially and then decreased, and the highest contents all appeared in 25.85% group, which were significantly higher than those of the other 3 groups (P < 0.05 or P < 0.01). It is concluded that properly increasing of dietary peNDF level is beneficial for improvement of rumen fluid pH and rumen fermentation, as well as the content of cellulolytic bacteria in rumen; under the conditions in the present study, 25.85% is the optimal peNDF_1.18 level in diets for 8 to 10-month-old heifers.

Key words： peNDF; feed intake; rumen fluid pH; ammonia nitrogen; volatile fatty acid; cellulolytic bacteria; heifers

Cite this article

CHEN Qing, WANG Hongrong, GE Rufang, CUI Huihui, WANG Mengzhi, YU Lihuai, LIN Miao . Effects of Dietary Physically Effective Neutral Detergent Fiber Level on Rumen Fermentation Parameters and Cellulolytic Bacteria of 8 to 10-Month-Old Heifers[J]. Chinese Journal of Animal Nutrition, 2015 , 27(4) : 1243 -1251 . DOI: 10.3969/j.issn.1006-267x.2015.04.031

References

[1] 王洪荣.反刍动物瘤胃酸中毒机制解析及其营养调控措施[J].动物营养学报, 2014, 26(10):3140-3148.

[2] MERTENS D R.Creating a system for meeting the fiber requirements of dairy cows[J].Journal of Dairy Science, 1997, 80(7):1463-1481.

[3] SCHWAB E C, SHAVER R D, SHINNERS K J, et al.Processing and chop length effects in Brown-Midrib corn silage on intake, digestion, and milk production by dairy cows[J].Journal of Dairy Science, 2002, 85(3):613-623.

[4] TAFAJ M, ZEBELI Q, BAES C, et al.A meta-analysis examining effects of particle size of total mixed rations on intake, rumen digestion and milk production in high-yielding dairy cows in early lactation[J].Animal Feed Science and Technology, 2007, 138(2):137-161.

[5] 张卫兵, 刁其玉, 赵广城.从营养和饲养角度加强后备牛的培育[J].乳业科学与技术, 2008(1):24-27.

[6] 刁其玉.优质后备奶牛的营养与培育[J].乳业科学与技术, 2009(03):101-105.

[7] 于永华, 纪小利.浅谈育成奶牛的饲养管理[J].中国畜禽种业, 2012, 12:94.

[8] 李文, 刘小林, 文利侠, 等.中国荷斯坦后备奶牛生长发育规律的研究[J].畜牧兽医杂志, 2007, 05:19-21, 24.

[9] NRC.Nutrient requirements of dairy cattle[S].7th ed.Washington, D.C.:National Academy Press, 2001.

[10] HEINRICHS A J, KONONOFF P J.Evaluating particle size of forages and TMRs using the Penn State Particle Separator[EB/OL].[2010-06-01].http://www.vetm ed.wsu.edu/courses-jmgay/ documents/DAS02421.pdf.

[11] 张宏福.动物营养参数与饲养标准[M].2版.北京:中国农业出版社, 2010:6.

[12] 冯宗慈, 高民.通过比色测定瘤胃液氨氮含量方法的改进[J].畜牧与饲料科学, 2010, 31(6-7):37.

[13] 解永磊, 秦松岩, 王昕彤, 毕成良.气相色谱测定挥发性脂肪酸的条件优化[J].广东农业科学, 2014, 23:98-101, 105.

[14] ZHOU J, BRUNS M A, TIEDJE J M.DNA recovery from soils of diverse composition[J].Applied and Environmental Microbiology, 1996, 62(2):316-322.

[15] 阳成波, 印遇龙, 黄瑞林, 等.实时定量RT-PCR的原理及方法[J].免疫学杂志, 2003, 19(3):145-150.

[16] MUYZER G, DE WAAL E C, UITTERLINDEN A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J].Applied and environmental microbiology, 1993, 59(3):695-700.

[17] 王加启.反刍动物营养学研究方法[M].北京:现代教育出版社, 2011:33.

[18] BEAUCHEMIN K A, YANG W Z, RODE L M.Effects of particle size of alfalfa-based dairy cow diets on chewing activity, ruminal fermentation, and milk production[J].Journal of Dairy Science, 2003, 86(2):630-643.

[19] YANG W Z, BEAUCHEMIN K A, RODE L M.Effects of particle size of alfalfa-based dairy cow diets on site and extent of digestion[J].Journal of Dairy Science, 2002, 85(8):1958-1968.

[20] YANG W Z, BEAUCHEMIN K A.Altering physically effective fiber intake through forage proportion and particle length:digestion and milk production[J].Journal of Dairy Science, 2007, 90(7):3410-3421.

[21] ALENDE M, DEPETRIS G J, DI MARCO O N, et al.Performance, chewing activity, and ruminal parameters in yearling beef steers fed early-harvested sorghum silage:effect of chop length and wheat straw addition[J].Animal Feed Science and Technology, 2009, 154(1-2):125-133.

[22] STOJANOVIC B, GRUBIC G, DJORDJEVIC N, et al.Effects of different levels of physically effective fibers in diets for cows in early lactation[J].Spanish Journal of Agricultural Research, 2012, 10(1):99-107.

[23] ALAMOUTI A A, ALIKHANI M, GHORBANI G R, et al.Effects of inclusion of neutral detergent soluble fibre sources in diets varying in forage particle size on feed intake, digestive processes, and performance of mid-lactation Holstein cows[J].Animal Feed Science and Technology, 2009, 154(1-2):9-23.

[24] YANG W Z, BEAUCHEMIN K A.Altering physically effective fiber intake through forage proportion and particle length:chewing and ruminal pH[J].Journal of Dairy Science, 2007, 90(6):2826-2838.

[25] ZHAO X H, ZHANG T, XU M, et al.Effects of physically effective fiber on chewing activity, ruminal fermentation, and digestibility in goats[J].Journal of Animal Science, 2011, 89(2):501-509.

[26] ZHAO X G, WANG M, TAN Z L, et al.Effects of Rice Straw Particle Size on Chewing Activity, Feed Intake, Rumen Fermentation and Digestion in Goats[J].Asian-Australasian Journal of Animal Sciences, 2009, 22(9):1256-1266.

[27] 冯仰廉.反刍动物营养学[M].北京:科学出版社, 2004.

[28] ZEBELI Q, TAFAJ M, JUNCK B, et al.Evaluation of the response of ruminal fermentation and activities of nonstarch polysaccharide-degrading enzymes to particle length of corn silage in dairy cows[J].Journal of Dairy Science, 2008, 91(6):2388-2398.

[29] BEAUCHEMIN K A, YANG W Z.Effects of physically effective fiber on intake, chewing activity, and ruminal acidosis for dairy cows fed diets based on corn silage[J].Journal of Dairy Science, 2005, 88(6):2117-2129.

[30] KONONOFF P J, HEINRICHS A J.The effect of reducing alfalfa haylage particle size on cows in early lactation[J].Journal of Dairy Science, 2003, 86(4):1445-1457.

[31] 贺鸣.TMR中粗饲料不同颗粒大小对干奶牛咀嚼行为和瘤胃发酵的影响[D].硕士学位论文.北京:中国农业大学, 2005.

[32] STORM A C, KRISTENSEN N B.Effects of particle size and dry matter content of a total mixed ration on intraruminal equilibration and net portal flux of volatile fatty acids in lactating dairy cows[J].Journal of Dairy Science, 2010, 93(9):4223-4238.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References