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Effects of Tea Saponin on Proliferation and Milk Fat Synthesis Key Enzymes of Bovine Mammary Epithelial Cells

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  • Key Laboratory for Dairy Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China

Received date: 2014-11-21

  Online published: 2015-04-21

Abstract

This experiment was conducted to study the effects of tea saponin on proliferation and milk fat synthesis key enzymes of bovine mammary epithelial cells. Mammary epithelial cells were obtained by the method of enzyme digestion. After immunofluorescence identification was done, mammary epithelial cells were cultured in tea saponin solution of different concentrations [0 (control), 0.05, 0.25, 0.50, 1.00, 5.00, 10.00, 20.00, 40.00, 60.00, 80.00 and 100.00 μg/mL]. 1) Thiazolyl blue tetrazolium bromide (MTT) method was used to detect the effects of tea saponin on the proliferation of cells. 2) Enzyme-linked immunosorbent assay (ELISA) kit was used to measure acetyl-CoA carboxylase (ACACA), fatty acid synthase (FASN) and stearoyl-coenzyme A desaturase (SCD) contents in cells. 3) Fluorescence-based quantitative real-time PCR method was used to measure the relative expression level of mRNA of ACACA, FASN and SCD. The results showed as follows: 1) compared with control group, there was no significant effect of tea saponin at concentration of 0.05 to 5.00 μg/mL (P>0.05), but the cell proliferation was significantly inhibited by tea saponin at concentration of 10.00 to 100.00 μg/mL (P<0.05); 2) the contents of FASN, ACACA and SCD in cells didn't significantly change among control group and 0.50, 5.00 and 20.00 μg/mL concentration groups (P>0.05); 3) compared with control group, the relative expression level of SCD mRNA in 0.50, 5.00 and 20.00 μg/mL concentration groups was significantly decreased (P<0.05). In conclusion, tea saponin plays a role in inhibition of proliferation and the relative expression level of fat synthesis key enzyme gene SCD mRNA of bovine mammary epithelial cells.

Cite this article

YAN Shuhong, XING Wenli, FANG Luoyun, WANG Junjie, LIU Xuhang, JIANG Linshu . Effects of Tea Saponin on Proliferation and Milk Fat Synthesis Key Enzymes of Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2015 , 27(4) : 1270 -1277 . DOI: 10.3969/j.issn.1006-267x.2015.04.034

References

[1] 孙妍.不同中短链与长链脂肪酸比例对奶牛乳脂含量及组成的影响[D].硕士学位论文.北京:中国农业科学院, 2012:5-42.

[2] 李俊, 张爱玉, 齐永杰, 等.茶树油粕中茶皂素研究进展[J].食品科学, 2012, 33(1):276-279.

[3] 张开慧.茶皂素的国内外研究进展[J].西部大开发, 2011(2):33-34.

[4] LAI Z F, SHAO Z Q, CHEN Y Z, et al.Effects of sasanquasaponin on ischemia and reperfusion injury in mouse hearts[J].Journal of Pharmacological Sciences, 2004, 94(3):313-324.  

[5] WANG J K, YE J A, LIU J X.Effects of tea saponins on rumen microbiota, rumen fermentation, methane production and growth performance-a review[J].Tropical Animal Health and Production, 2012, 44(4):697-706.  

[6] 叶均安, 刘建新, 板桥久雄.茶皂素对瘤胃原虫的抑制效果[J].中国饲料, 2001, 1(2):30-32.

[7] 来海良, 王一义.茶皂素对湖羊瘤胃培养物发酵的影响[J].现代农业科技, 2010(23):300-302.

[8] 苑文珠.茶皂素对湖羊生产性能及瘤胃发酵的影响[D].硕士学位论文.杭州:浙江大学, 2002:1-32.

[9] 彭春雨, 孟庆翔, 任丽萍, 等.植物提取物对奶牛产奶性能和血液指标的影响[J].饲料研究, 2011(7):8-10.

[10] 王亨, 吴培福, 邱昌伟, 等.荷斯坦奶牛乳腺上皮细胞的体外培养[J].畜牧与兽医, 2007, 39(4):41-43.

[11] 李喜艳, 王加启, 魏宏阳, 等.奶牛乳腺上皮细胞的体外培养方法及其应用概述[J].华北农学报, 2010, 25(增刊):31-35.

[12] HU H, WANG J Q, BU D P, et al.In vitro culture and characterization of a mammary epithelial cell line from Chinese Holstein dairy cow[J].PLoS One, 2009, 4(11):e7636.

[13] TONG H L, LI Q Z, GAO X J, et al.Establishment and characterization of a lactating dairy goat mammary gland epithelial cell line[J].In Vitro Cellular & Developmental Biology-Animal, 2012, 48(3):149-155.  

[14] 陈建晖, 佟慧丽, 李庆章, 等.奶牛乳腺上皮细胞系的建立[J].畜牧兽医学报, 2009, 40(5):743-747.

[15] 朱丽丽, 李惠芬.重楼皂苷对SGC-7901细胞增殖抑制及诱导凋亡的实验研究[J].时珍国医国药, 2009, 20(6):1501-1502.

[16] 吴葆华, 陈喆, 吕望, 等.桔梗皂苷D抑制人结肠癌SW620细胞增殖及其机制的研究[J].中国药学杂志, 2013, 48(增刊):354-358.

[17] 彭春燕.苷类物质对山羊乳腺上皮细胞增殖及酪蛋白基因表达的影响[D].硕士学位论文.江苏:扬州大学, 2011:2-54.

[18] 王红芳.外源反-10, 顺-12共轭亚油酸对牛乳腺上皮细胞脂肪合成的影响及其分子机制[D].硕士学位论文.泰安:山东农业大学, 2011:8-60.

[19] 庞坤, 韩立强.油脂对反刍动物乳腺脂肪关键合成酶分子调控的研究进展[J].中国农学通报, 2012, 28(8):1-5.

[20] 茅慧玲, 陈雪君, 林嘉, 等.日粮中添加茶皂素和豆油对羔羊生长性能以及肌肉脂肪酸的影响[C]//中国畜牧兽医学会动物营养学分会第十次学术研讨会论文集.北京:中国畜牧兽医学会动物营养学分会, 2008, 327-330.

[21] 王延芳.油茶皂素及其水解产物的分离及降血脂抗氧化活性研究[D].硕士学位论文.广州:华南理工大学, 2012:5-74.

[22] 夏春华, 杨钟鸣, 朱伯荣, 等.茶皂素在农药工业中应用研究进展[J].茶叶科学, 2000, 20(2):82-88.

[23] 张国运, 曹丽云, 吴建鹏, 等.茶皂素提取工艺及其应用研究进展[J].日用化学工业, 2006, 36(3):174-177.

[24] 杨强, 张石蕊.茶皂素在动物生产中的应用[J].中国饲料, 2007(8):8-10.

[25] 周奕毅.茶皂素抑制湖羊甲烷生成的微生物学机制研究[D].硕士学位论文.杭州:浙江大学, 2009:1-66.

[26] HU W L, LIU J X, YE J A, et al.Effect of tea Saponin on rumen fermentation in vitro[J].Animal Feed Science and Technology, 2005, 120(3/4):333-339.

[27] 王加启.牛奶乳脂肪和乳蛋白的合成与调控机理[J].饲料与畜牧, 2011(2):8-14.
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