Cloning and Sequence Analysis of Full Length cDNAs of Growth Hormone/Insulin-Like Growth Factor-Ⅰ Axis Relative Genes of Japanese Seabass (Lateolabrax japonicus) and Siberian Sturgeon (Acipenser baerii Brandt)

ZHANG Zhiyong, XUE Min, WANG Jia, WU Xiufeng, ZHENG Yinhua, HAN Fang

doi:10.3969/j.issn.1006-267x.2015.04.035

2015 , Vol. 27 >Issue 4: 1278 - 1292

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.04.035

Short Communications

Cloning and Sequence Analysis of Full Length cDNAs of Growth Hormone/Insulin-Like Growth Factor-Ⅰ Axis Relative Genes of Japanese Seabass (Lateolabrax japonicus) and Siberian Sturgeon (Acipenser baerii Brandt)

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1. The National Aquatic Feed Safety Assessment Center, Feed Institute Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. Animal Nutrition and Feed Center, Food Nutrition and Health Research Institute Co., Ltd., Beijing 102209, China;
3. Key Laboratory of Feed Biotechnology, The Ministry of Agriculture, Beijing 100081, China

Received date: 2014-10-29

Online published: 2015-04-21

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Abstract

The reverse transcription-phosphatase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods were used in this experiment to clone the full-length sequences of cDNAs for growth hormone (GH) and GH receptor (GHR) of Japanese seabass (Lateolabrax japonicus) and Siberian sturgeon (Acipenser baerii Brandt). The results showed as follows: the full-length GH cDNA of Japanese seabass (GenBank accession No. JQ995145) was 949 bp, consisting of an open reading frame (ORF) of 615 bp encoding a protein of 204 amino acids with a theoretical molecular weight of 23.06 ku, and shared 36.0% to 90.2% similarity with other species. The full-length GHRⅠ cDNA of Japanese seabass (GenBank accession No. JX402001) was 3 070 bp, consisting of an ORF of 1 911 bp encoding a protein of 637 amino acids with a theoretical molecular weight of 70.79 ku, and shared 32.2% to 86.2% similarity with other species. The full-length GHRⅡ cDNA of Japanese seabass (GenBank accession No. JQ995146) was 2 926 bp, consisting of an ORF of 1 749 bp encoding a protein of 582 amino acids with a theoretical molecular weight of 64.42 ku, and shared 30.8% to 81.4% similarity with other species. The full-length GH cDNA of Siberian sturgeon (GenBank accession No. JX003684) was 999 bp, consisting of an ORF of 645 bp encoding a protein of 214 amino acids with a theoretical molecular weight of 24.14 ku, and shared 38.3% to 70.1% similarity with other species. The full-length GHR cDNA of Siberian sturgeon (GenBank accession No. JX003685) was 2 238 bp, consisting of an ORF of 1 716 bp encoding a protein of 571 amino acids with a theoretical molecular weight of 63.41 ku, and shared 39.5% to 49.2% with other species. The full-length sequences of cDNAs for GH and GHR of Japanese seabass and Siberian sturgeon will provide a scientific basis for further study on the regulatory mechanism of GH/IGF-Ⅰ axis on growth and development of fish.

Key words： Japanese seabass (Lateolabrax japonicus); Siberian sturgeon (Acipenser baerii Brandt); gene cloning; GH/IGF-Ⅰ axis; growth hormone; growth hormone receptor

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ZHANG Zhiyong, XUE Min, WANG Jia, WU Xiufeng, ZHENG Yinhua, HAN Fang . Cloning and Sequence Analysis of Full Length cDNAs of Growth Hormone/Insulin-Like Growth Factor-Ⅰ Axis Relative Genes of Japanese Seabass (Lateolabrax japonicus) and Siberian Sturgeon (Acipenser baerii Brandt)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(4) : 1278 -1292 . DOI: 10.3969/j.issn.1006-267x.2015.04.035

References

[1] 钟欢.GH/IGF轴相关基因在不同倍性鲫鲤中的表达及与生长的相关性研究[D].博士学位论文.长沙:湖南师范大学, 2012.

[2] 张志勇, 薛敏, 王嘉, 等.混合植物蛋白质替代鱼粉对花鲈和西伯利亚鲟生长和肉质影响的比较研究[J].动物营养学报, 2013, 25(6):1260-1275.

[3] ROSE T M, SCHULTZ E R, HENIKOFF J G, et al.Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences[J].Nucleic Acids Research, 1998, 26(7):1628-1635.

[4] KUMAR S, TAMURA K, JAKOBSEN I B, et al.MEGA2:molecular evolutionary genetics analysis software[J].Bioinformatics, 2001, 17(12):1244-1245.

[5] PÊREZ-SÁNCHEZ J, LE BAIL P Y.Growth hormone axis as marker of nutritional status and growth performance in fish[J].Aquaculture, 1999, 177(1/2/3/4):117-128.

[6] AYSON F G, DE JESUS E G T, AMEMIYA Y, et al.Isolation, cDNA cloning, and growth promoting activity of rabbitfish (Siganus guttatus) growth hormone[J].General and Comparative Endocrinology, 2000, 117(2):251-259.

[7] 马细兰, 张勇, 黄卫人, 等.尼罗罗非鱼生长激素及其受体的cDNA克隆与mRNA表达的雌雄差异[J].动物学报, 2006, 52(5):924-933.

[8] 李胜杰, 白俊杰, 叶星, 等.加州鲈生长激素和胰岛素样生长因子I cDNA的克隆及序列分析[J].广东海洋大学学报, 2007, 27(3):1-5.

[9] GARDINER B G.Sturgeons as living fossils[M]//ELDREDGE N, STANLEY S M.Living fossils.New York:Springer Verlag Press, 1984:148-152.

[10] CALDUCH-GINER J A, MINGARRO M, DE CELIS S V R, et al.Molecular cloning and characterization of gilthead sea bream (Sparus aurata) growth hormone receptor (GHR).Assessment of alternative splicing[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 2003, 136(1):1-13.

[11] VERY N M, KITTILSON J D, NORBECK L K, et al.Isolation, characterization, and distribution of two cDNAs encoding for growth hormone receptor in rainbow trout (Oncorhynchus mykiss)[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 2005, 140(4):615-628.

[12] SAERA-VILA A, CALDUCH-GINER J A, PÉREZ-SÁNCHEZ J.Duplication of growth hormone receptor (GHR) in fish genome:gene organization and transcriptional regulation of GHR type Ⅰ and Ⅱ in gilthead sea bream (Sparus aurata)[J].General and Comparative Endocrinology, 2005, 142(1/2):193-203.

[13] 章力, 黄希贵, 焦保卫, 等.南方鲇两种生长激素受体的结构分析及其组织分布和激素调节[J].动物学报, 2006, 52(6):1096-1106.

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