Difference Analysis of Gut Microbiome of Rhinopithecus roxellana in Different Ages

JIAN Ping, WANG Qiang, WANG Jian, NIU Lili, ZHU Hui, ZENG Yan, NI Xueqin, ZENG Dong

doi:10.3969/j.issn.1006-267x.2015.04.037

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 4: 1302 - 1309

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.04.037

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Difference Analysis of Gut Microbiome of Rhinopithecus roxellana in Different Ages

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1. Research Center of Animal Micro-Ecological, College of Veterinary, Sichuan Agricultural University, Ya'an 625014, China;
2. Chengdu Zoo, Chengdu 610080, China;
3. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya'an 625014, China

Received date: 2014-10-29

Online published: 2015-04-21

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Abstract

This experiment aimed at analyzing the structure of gut microbiome in young, adult and old Rhinopithecus roxellana, and analyzing the change trend of the gut microbiome with the age increasing. Performed the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) with the total DNA extracted from the faeces of young, adult and old Rhinopithecus roxellana, and the results were analyzed by UPGMA cluster and principal component, and the biological diversity of Rhinopithecus roxellana in different ages was compared. The number of eubacteria, Lactobacillus spp., Enterobacteriaceae, Bifidobacterium spp. in gut microbiome was detected by fluorescence quantitative PCR technology. The results showed as follows: not only the UPGMA cluster analysis, but also the principal component analysis and the biological diversity comparison indicated that the diversity of gut microbiome of Rhinopithecus roxellana in different ages existed obvious differences. The advantages of the band of the young and adult Rhinopithecus roxellana were more than the old Rhinopithecus roxellana in the fingerprint. The number of eubacteria in the gut microbiome of Rhinopithecus roxellana in different ages was all in the level 10¹¹. The number of Enterobacteriaceae in the gut microbiome of the young Rhinopithecus roxellana was significantly less than the adult's and the old's (P<0.05). The number of Lactobacillus spp. in the gut microbiome of the adult Rhinopithecus roxellana was significantly more than the young's and the old's (P<0.01). The number of Bifidobacterium spp. in the gut microbiome of the old Rhinopithecus roxellana was significantly less than the adult's and the young's (P<0.01). In conclusion, along with the age increasing, the diversity of the gut microbiome of Rhinopithecus roxellana shows an increasing trend from childhood to adulthood and shows a reducing trend from adulthood to senectitude. And, the number of advantage bacterium group is reduced in the senile period. Moreover, the number of gut microbiome of Rhinopithecus roxellana has great differences in different ages.

Key words： Rhinopithecus roxellana; age; gut microbiome; fluorescence quantitative PCR; PCR-DGGE

Cite this article

JIAN Ping, WANG Qiang, WANG Jian, NIU Lili, ZHU Hui, ZENG Yan, NI Xueqin, ZENG Dong . Difference Analysis of Gut Microbiome of Rhinopithecus roxellana in Different Ages[J]. Chinese Journal of Animal Nutrition, 2015 , 27(4) : 1302 -1309 . DOI: 10.3969/j.issn.1006-267x.2015.04.037

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