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Isolation, Identification and Acclimation of Lactobacillus acidophilus from Intestinal Tracts of Piglets

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  • College of Bioscience and Bioengineering of Jiangxi Agricultural University, Nanchang Key Laboratory of Fermentation Application Technology, Nanchang 330045, China

Received date: 2014-11-30

  Online published: 2015-04-21

Abstract

The paper aimed at isolation and screening of a strain, Lactobacillus acidophilus, which can be localized well in intestinal tracts of pigs. Fresh feces of piglets were selected for bacteria source, MRS medium was used, and streak plate techniques were applied for isolation and purification. Bacteria were identified through morphological observation, biochemical and physiological tests, as well as 16S rRNA gene sequence analyis. Bacteria strains were acclimated in gradually elevated acidity and concentration of bile salt in medium and culture temperatures. The results showed as follows: 1) a strain named La-5 was isolated and purified, which was identified as Lactobacillus acidophilus. 2) Strain La-5c screened from original strain La-5 by adapting had a 36% to 44% increment of survival rate compared with the original strain La-5 under the range of pH 1.5 to 3. The strain La-5 had a survival rate of 37% at pH=1.5. 3) In the range of 0.03% to 0.3% of bile salt concentration, strain La-5c showed a survival rate of 85% to 90%, while strain La-5 only had 28% to 53%. 4) At the culture temperature of 50 ℃, survival rate of strain La-5c was 66.1%, but it is <0.01% for the original strain La-5. It is concluded that the acclimated strain La-5c had elevated tolerances to low pH, high concentration of bile salt and high culture temperature compared with the original Lactobacillus acidophilus La-5 from piglet feces.

Cite this article

HU Guocai, HUANG Jing, WU Xiaoyu . Isolation, Identification and Acclimation of Lactobacillus acidophilus from Intestinal Tracts of Piglets[J]. Chinese Journal of Animal Nutrition, 2015 , 27(4) : 1316 -1325 . DOI: 10.3969/j.issn.1006-267x.2015.04.039

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