Aquaculture Nutrition

Effects of Dietary Dextrin Level on Growth and Carbohydrate Metabolism of Ukraine Scaly Carp (Cyprinus carpio)

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  • Department of Fishery Science, Tianjin Agricultural University, Tianjin 300384, China

Received date: 2014-12-23

  Online published: 2015-05-13

Abstract

In order to investigate the effects of dietary dextrin level on growth and carbohydrate metabolism of Ukraine scaly carp (Cyprinus carpio), Ukraine scaly carp with an average body weight about of 3.92 g were used as test object, and 2 experimental diets with 30% protein level were designed which containing 15% and 25% dextrin, respectively. A total of 300 Ukraine scaly carp were randomly divided into 2 groups with 3 replicates per group and 50 fish per replicate. After 8 weeks feeding, the growth indices, biochemical indices and carbohydrate metabolize enzyme activities of Ukraine scaly carp were examined. Glucokinase (GK) and glucose-6-phosphatase (G6Pase) mRNA expression levels in hepatopancreas and intestine during the refeeding after fasting 48 h were tested. The results showed as follows: 1) specific growth rate in the 25% dextrin group was significantly higher than that in the 15% dextrin group (P<0.05). 2) The contents of serum triglyceride, cholesterol and glucose in the 25% dextrin group were significantly higher than those in the 15% dextrin group (P<0.05). 3)The activities of serum hexokinase (HK), GK, pyruvate kinase (PK) and malate dehydrogenase (MDH) and hepatopancreas HK, GK and G6Pase in the 25% dextrin group were significantly higher than those in the 15% dextrin group (P<0.05), while the activity of hepatopancreas MDH was significantly lower than that in the 15% dextrin group (P<0.05). 4) As for the GK mRNA expression level in hepatopancreas at 24 h after refeeding, the 25% dextrin group was significantly higher than the 15% dextrin group (P<0.05);compared with 15% dextrin, the G6Pase mRNA expression level in hepatopancreas was significantly increased by 25% dextrin at 6 h after refeeding (P<0.05). The results indicate that the improvement effect of 25% of dietary dextrin level for growth and carbohydrate metabolism of Ukraine scaly carp is better than 15% of dietary dextrin level under this experimental condition.

Cite this article

GAO Yan, LI Jinghui, FANG Zhenzhen, CHENG Zhenyan, QIAO Xiuting, BAI Dongqing . Effects of Dietary Dextrin Level on Growth and Carbohydrate Metabolism of Ukraine Scaly Carp (Cyprinus carpio)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(5) : 1401 -1410 . DOI: 10.3969/j.issn.1006-267x.2015.05.010

References

[1] ZHOU C,LIU B,GE X,et al.Effect of dietary carbohydrate on the growth performance,immune response,hepatic antioxidant abilities and heat shock protein 70 expression of Wuchang bream,Megalobrama amblycephala[J].Journal of Applied Ichthyology,2013,29(6):1348-1356.  

[2] 窦兵帅,梁萌青,郑珂珂,等.饲料中碳水化合物水平对鲈鱼生长、生理状态参数及体组成的影响[J].渔业科学进展,2014,35(1):46-54.

[3] LIU B,XIE J,GE X P,et al.Effect of high dietary carbohydrate on growth,serum physiologicalresponse,and hepatic heat shock cognate protein 70 expressionof the top-mouth culter Erythroculter ilishaeformis Bleeker[J].Fisheries Science,2012,78(3):613-623.  

[4] TIAN L X,LIU Y J,YANG H J,et al.Effects of different dietary wheat starch levels on growth,feed efficiency and digestibility in grass carp (Ctenopharyngodon idella)[J].Aquaculture International,2012,20(2):283-293.  

[5] ENES P,PERES H,COUTO A, et al.Growth performance and metabolic utilization of diets including starch,dextrin,maltose or glucose as carbohydrate source by gilthead sea bream (Sparus aurata) juveniles[J].Fish Physiology Biochemistry,2010,36(4):903-910.  

[6] 聂琴,苗惠君,苗淑彦,等.不同糖源及糖水平对大菱鲆糖代谢酶活性的影响[J].水生生物学报,2013,37(3):425-433.

[7] 商华.优良养殖新品种——乌克兰鳞鲤[J].农村百事通,2013(6):45-46,81.

[8] 王金雨.乌克兰鳞鲤二、三倍体部分生物学性状的比较研究[D].硕士学位论文.天津:天津农学院,2010.

[9] 桂远明.水产动物动物机能学实验[M].北京:中国农业出版社,2004:113-116,119-124.

[10] PANSERAT S,FONTAGNÉ S,BERGOT P,et al.Ontogenesis of hexokinase Ⅰ and hexokinaseⅣ (glucokinase) gene expressions in common carp (Cyprinus carpio) related to diet[J].British Journal of Nutrition,2001,85(6):649-651.  

[11] 王广宇.日粮碳水化合物水平对翘嘴红鲌生长、血液指标及GKG6PaseHSC70基因表达的影响[D].硕士学位论文.南京:南京农业大学,2009.

[12] 程汉良,姬南京,彭永兴,等.彭泽鲫葡萄糖激酶基因全长cDNA克隆及表达分析[J].动物营养学报,2011,23(7):1167-1175.

[13] 刘襄河,叶超霞,郑丽勉,等.饲料糊精水平对暗纹东方鲀幼鱼生长、消化酶活性和血液生化指标的影响[J].水产学报,2013,37(9):1359-1368.

[14] 蒋利和,吴宏玉,黄凯,等.饲料糖水平对吉富罗非鱼幼鱼生长和肝代谢功能的影响[J].水产学报,2013,37(2):245-255.

[15] 许明珠,张琴,童万平,等.饲料糖水平对方格星虫稚虫生长、体组成和消化酶活性的影响[J].动物营养学报,2013,25(3):534-542.

[16] YI T,SUN J,LIANG X,et al.Effects of polymorphisms in pepsinogen (PEP),amylase (AMY) and trypsin (TRY) genes on food habit domestication traits in mandarin fish[J].International Journal of Molecular Sciences,2013,14(11):21504-21512.  

[17] MARCUSCHI M,ESPOSITO T,MACHADO M,et al.Purification,characterization and substrate specificity of a trypsin from the Amazonian fish tambaqui (Colossoma macropomum)[J].Biochemical and Biophysical Research Communications,2010,396(3):667-673.  

[18] 强俊,王辉,彭俊,等.饲料碳水化合物水平对奥尼罗非鱼仔稚鱼生长的影响[J].饲料工业,2009,30(14):32-35.

[19] KROGDAHL Å,HEMRE G I,MOMMSENT P.Carbohydrates in fish nutrition:digestion and absorption in post larval stages[J].Aquaculture Nutrition,2005,11(2):103-122.  

[20] 任鸣春,贾文锦,戈贤平,等.饲料不同淀粉水平对团头鲂成鱼生长性能、消化酶活性及肌肉成分的影响[J].水产学报,2014,38(9):1494-1502.

[21] 范国燕,李英文.饥饿胁迫对南方鲇幼鱼糖代谢的影响[J].重庆师范大学学报:自然科学版,2011,28(3):22-27.

[22] BOUCHARD-MERCIER A,RUDKOWSKA I,Lemieux S,et al.An interaction effect between glucokinase gene variationand carbohydrate intakes modulates the plasma triglycerideresponse to a fish oil supplementation[J].Genes and Nutrition,2014,9(3):395.  

[23] 王镜岩.生物化学:下册[M].3版.北京:高等教育出版社,2002.

[24] 汪福保,罗莉,文华,等.镁对草鱼生长、形体、肝功能和糖代谢的影响[J].淡水渔业,2011,41(2):57-62,68.

[25] 张颂,蒋明,文华,等.饲料碳、脂比例对胭脂鱼幼鱼生长及糖代谢的影响[J].华南农业大学学报,2014,35(3):1-7,23.

[26] 张世亮,艾庆辉,徐玮,等.饲料中糖/脂肪比例对瓦氏黄颡鱼生长、饲料利用、血糖水平和肝脏糖酵解酶活力的影响[J].水生生物学报,2012,36(3):466-473.

[27] KUMAR V,SAHU N P,PAL A K,et al.Modulation of key enzymes of glycolysis,gluconeogenesis,amino acid catabolism,and TCA cycle of the tropical freshwater fish Labeo rohita fed gelatinizedand non-gelatinized starch diet[J].Fish Physiology and Biochemistry,2010,36(3):491-499.  

[28] 刘波,唐永凯,俞菊华,等.饲料脂肪对翘嘴红鲌生长、葡萄糖激酶和葡萄糖-6-磷酸酶活性与基因表达的影响[J].中国水产科学,2008,15(6):1024-1033.

[29] ENES P,PANSERAT S,KAUSHIK S,et al.Effect of normal and waxy maize starch on growth,food utilization and hepatic glucose metabolism in European sea bass (Dicentrarchus labrax) juveniles[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2006,143(1):89-96.  

[30] ENES P,PANSERAT S,KAUSHIK S,et al.Hepatic glucokinase and glucose-6-phosphatase responses to dietary glucose and starch in gilthead sea bream (Sparus aurata)juveniles reared at two temperatures[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2008,149(1):80-86.  

[31] POLAKOF S,SKIBA-CASSY S,KAUSHIK S,et al.Glucose and lipid metabolism in the pancreas of rainbow trout is regulated at the molecular level by nutritional status and carbohydrate intake[J].Journal of Comparative Physiology Part B:Biochemical Systemic and Environmental Physilogy,2012,182(4):507-516.

[32] 杨莹.日粮糖水平对瓦氏黄颡鱼幼鱼生长和糖代谢相关酶基因表达的影响[D].硕士学位论文.上海:华东师范大学,2011.

[33] 戈贤平.不同糖、脂含量日粮对翘嘴红鲌相关糖代谢酶的调节研究[D].博士学位论文.南京:南京农业大学,2006.

[34] PANSERAT S,PLAGNES-JUAN E,KAUSHIK S.Gluconeogenic enzyme gene expressions is decreased by dietary carbohydrate in common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata)[J].Biochimica et Biophysica Acta:Gene Structure and Expression,2002,1579(1):35-42.  
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