Evaluation of Enzymatic Hydrolysate Gross Energy of Glyphosate-Tolerant Corn and Transgenic Bt Corn Using Simulative Digestion System

ZHONG Ruqing, CHEN Liang, GAO Lixiang, HUANG Qinghua, LIU Lei, ZHANG Hongfu

doi:10.3969/j.issn.1006-267x.2015.05.017

2015 , Vol. 27 >Issue 5: 1468 - 1476

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.05.017

Experimental Methods

Evaluation of Enzymatic Hydrolysate Gross Energy of Glyphosate-Tolerant Corn and Transgenic Bt Corn Using Simulative Digestion System

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State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2014-12-09

Online published: 2015-05-13

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Abstract

The objective of the present study was to evaluate the energy value of the glyphosate-tolerant corn and transgenic Bt corn or the glyphosate-tolerant corn diet and transgenic Bt corn diet comparing to the isogenic non-transgenic corn or diet using an in vitro digestion method with computer-controlled simulated digestion system (SDS). It would provide a reference to develop in vitro method for evaluating the nutritional substantial equivalence of genetically modified crops using the SDS. A single factorial completely randomized design with 3 corn ingredients (isogenic non-transgenic corn, glyphosate-tolerant corn, and transgenic Bt corn) and corresponding 3 corn diets (isogenic non-transgenic corn diet, glyphosate-tolerant corn diet, and transgenic Bt corn diet) was adapted. The digestion in the stomach and total tract were simulated respectively. The in vitro dry matter digestibility (DMD), in vitro gross energy digestibility (GED), and enzymatic hydrolysate gross energy (EHGE) were determined of corn ingredients and diets. The results showed that the nutrient concentration was similar between the corn ingredients and diets. As a whole, the gastric and total tract DMD, the gastric and total tract GED and the EHGH were not affected by the corn ingredient treatments (P>0.05). However, the GED of transgenic Bt corn were lower than the GED of isogenic non-transgenic corn (P=0.03, coefficient of variation=0.50%), when the EHGE of transgenic Bt corn diet were greater than the EHGE of isogenic non-transgenic corn diet (P=0.02, coefficient of variation=1.12%). But, above two coefficient of variations were all less than the coefficient of variation of feed ingredients using the SDS (coefficient of variation≤1.64%). These results suggest that in vitro gastric and total tract digestibility of dry matter and gross energy for glyphosate-tolerant corn is similar to that for isogenic non-transgenic corn, with transgenic Bt corn has some statistical differences. These differences are all within the test error of the SDS, via next animal testing test and verify. The SDS may supply a new method for evaluating the nutritional substantial equivalence of genetically modified crops.

Key words： transgenic maize; in vitro gross energy digestibility; in vitro dry matter digestibility; enzymatic hydrolysate gross energy; simulative digestion system

Cite this article

ZHONG Ruqing, CHEN Liang, GAO Lixiang, HUANG Qinghua, LIU Lei, ZHANG Hongfu . Evaluation of Enzymatic Hydrolysate Gross Energy of Glyphosate-Tolerant Corn and Transgenic Bt Corn Using Simulative Digestion System[J]. Chinese Journal of Animal Nutrition, 2015 , 27(5) : 1468 -1476 . DOI: 10.3969/j.issn.1006-267x.2015.05.017

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