This test was conducted to research the quality of fermented feed by the feruloyl esterases, and investigate the effects of it on the performance of broilers. This paper was conducted to reduce the existed non-starch polysaccharides anti-nutritional factors in the feed and promote the intake of the nutrients. A total of 180 yellow foot chicken aged of 20 days were allocated to 4 groups with 5 replicates per group and 9 chicken per replicate. The 4 groups were fed basal diet with 10% stipe (control), 10% common fermented feed, 10% enzymatic feed and 10% enzymatic fermentation feed. The results showed that, the contents of crude protein, total amino acids, ferulic acid and xylooligosaccharides, and the number of total beneficial bacteria in enzymatic fermented feed were higher than those of the common fermented feed. In the broiler's feeding, average daily gain of broilers was increased by 6.42%, average daily feed intake and feed to gain ratio were decreased by 5.91% and 11.34%, respectively. Our results demonstrate the feruloyl esterases enzymatic fermented feed can improve the feed quality, promote the broiler growth and enhance the feed conversion rate.
WANG Linlin, CHEN Peiqin, LUO Yun, LI Xialan
. Enzymatic fermented feed by the Feruloyl Esterases: Quality and Effects on the Performance of Broilers[J]. Chinese Journal of Animal Nutrition, 2015
, 27(5)
: 1540
-1548
.
DOI: 10.3969/j.issn.1006-267x.2015.05.026
[1] 李夏兰,程珊影,杨道秀,等.阿魏酸酯酶和木聚糖酶协同降解麦糟[J].化工进展,2012,31(5):1096-1102.
[2] YANG H J,XIE C Y.Assessment of fibrolytic activities of 18 commercial enzyme products and their abilities to degrade the cell wall fraction of corn stalks in in vitro enzymatic and ruminal batch cultures[J].Animal Feed Science and Technology,2010,159(3):110-121.
[3] 赵东平,杨文钰,陈兴福.阿魏酸的研究进展[J].时珍国医国药,2008,19(8):1839-1841.
[4] 陈瑞娟.新型低聚糖的介绍[J].食品与发酵工业,2003(2):82-90.
[5] 中华人民共和国农业部.NY/T 33—2004 鸡饲养标准[S].北京:中国标准出版社,2004.
[6] 邓轶韬,李夏兰,陈宗香,等.阿魏酸酯酶产生菌的筛选及产酶条件的优化[J].华侨大学学报:自然科学版,2011,32(3):300-303.
[7] 中华人民共和国国家技术监督局.GB/T 6432—1994.饲料中粗蛋白测定方法[S].北京:中国标准出版社,1995.
[8] 中华人民共和国国家质量监督检验检疫总局.GB/T 20806—2006饲料中中性洗涤纤维(NDF)的测定[S].北京:中国标准出版社,2006.
[9] 中华人民共和国农业部.NY/T 1459—2007饲料中酸性洗涤纤维的测定[S].北京:中国标准出版社,2007.
[10] 中华人民共和国国家质量监督检验检疫总局.GB/T 20805—2006饲料中酸性洗涤木质素的测定[S].北京:中国标准出版社,2006.
[11] 邢颖.氨基酸金属离子螯合物合成条件及测定方法的研究[D].硕士学位论文.成都:四川大学,2011.
[12] 孙晶,朱学伸,王苑,等.食品中L-乳酸检测方法及其研究进展[J].安徽农业科学,2010,38(31):17801-17802.
[13] 中华人民共和国国家质量监督检验检疫总局.GB/T 13093—2006饲料中细菌总数的测定[S].北京:中国标准出版社,2006.
[14] 中华人民共和国国家卫生部.GB 4789.35—2010 食品安全国家标准 食品微生物学检验 乳酸菌检验[S].北京:中国标准出版社,2010.
[15] 周乃继,李爱科,张晓琳,等.一种用于饲料肽快速检测的Tricine-SDS-PAGE方法[J].中国生物工程杂志,2008(专刊):63-64.
[16] 中华人民共和国国家质量监督检验检疫总局.GB 13078—2001饲料卫生标准[S].北京:中国标准出版社,2001.
[17] 中华人民共和国国家质量监督检验检疫总局.GB 18406.3—2001农产品安全质量无公害畜禽肉安全要求[S].北京:中国标准出版社,2001.
[18] 胡瑞,陈艳,王之盛,等.复合益生菌发酵豆粕生产工艺参数的优化及酶菌联合发酵对豆粕品质的影响[J].动物营养学报,2013,25(8):1896-1903.
[19] 李龙,陈小连,徐建雄.复合益生菌发酵饲料工艺参数及其品质研究[J].上海交通大学学报:农业科学版,2010,28(6):530-551.
[20] 贾朋辉,郭洪新,李国军,等.微生态发酵饲料菌群变化及其应用[J].饲料博览,2009(3):24-27.
[21] 杨道秀,李夏兰,陈培钦,等.新型复合酶在肉鸡饲料中的应用[J].食品与生物技术学报,2013,32(4):410-416.
[22] 杨红建,谢春元.一种饲用复合阿魏酸酯酶添加剂[P].CN101228921,中国专利.2008-07-30.
[23] KRUEGER N A,ADESOGAN A T,STAPLES C R,et al.The potential to increase digestibility of tropical grasses with a fungal,ferulic acid esterase enzyme preparation[J].Animal Feed Science and Technology,2008,145(1):95-108.