Short Communications

Effect of Sodium Nitrate and 2-Bromoethanesulphonate on Methane, Hydrogen and Volatile Fatty Acids Production of in Vitro Ruminal Fermentation

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  • 1. Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha 410125, China;
    2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    3. Chinese Academy of Agricultural Engineering, Beijing 100125, China;
    4. College of Horticulture Landscape, Hunan Agricultural University, Changsha 410128, China

Received date: 2014-11-25

  Online published: 2015-05-13

Abstract

The aim of this study was to investigate the mechanism of sodium nitrate (NaNO3) and 2-bromoethanesulphonate (BES) on methane inhibition from the biochemical aspect of ruminal hydrogen metabolism. Rumen fluid was collected from two adult Xiangdong black goats with permanent rumen fistulas. The study contained control group, NaNO3 groups (supplemental level was 0.10, 0.20 and 0.40 mg/mL, respectively) and BES groups (supplemental level was 0.63, 1.26 and 2.52 mg/mL, respectively). The 24 h in vitro ruminal incubation was performed to obtain gas production, production and contents of hydrogen and methane, and volatile fatty acids production and composition using automatic fermentation equipment. The results showed as follows: NaNO3 and BES supplementation significantly lowered methane production in comparison with control group (P<0.05), and increasing supplemental level of NaNO3 and BES linearly decreased methane production (P<0.05). The lower supplemental level of NaNO3 (≤0.20 mg/mL) had no significant effects on gas production, initial fractional rate of degradation (FRD0), acetate and propionate production and the ratio of acetate to propionate (P>0.05), while the higher supplemental level of NaNO3 (0.40 mg/mL) had significantly higher hydrogen production (P<0.05), and significantly lower gas production, methane production, FRD0 and total volatile fatty acids production in comparison with control group (P<0.05). BES had no significant effects on hydrogen production, FRD0 and total volatile fatty acids production in comparison with control group (P>0.05), but had significantly higher hydrogen production and content (P<0.05), and decreased the ratio of acetate to propionate (P<0.05). BES could alter pattern of rumen fermentation through facilitating propionate production. In conclusion, NaNO3 reduces methane production through hydrogen sink and rumen microbial toxicity, while BES reduced methane production through inhibiting the activity of methanogens, and had little effects on gas production and substrate degradation.

Cite this article

WANG Rong, TAN Liwei, WANG Min, DENG Jinping, YAN Zhicheng, WANG Yushi, TAN Zhiliang . Effect of Sodium Nitrate and 2-Bromoethanesulphonate on Methane, Hydrogen and Volatile Fatty Acids Production of in Vitro Ruminal Fermentation[J]. Chinese Journal of Animal Nutrition, 2015 , 27(5) : 1586 -1595 . DOI: 10.3969/j.issn.1006-267x.2015.05.031

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