Experimental Methods

Isolation and Expression Patterns of Japonicin-pb from Paa boulengeri

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  • 1. College of Life Science & Forestry, Chongqing University of Art & Science, Chongqing 402160, China;
    2. Chongqing Research Centers of Conservation & Development on Rare & Endangered Aquatic Resources, Chongqing 402168, China

Received date: 2014-12-23

  Online published: 2015-05-13

Abstract

In order to provided clues to the development of Japonicin-pb, we cloned the Japonicin-pb from Paa boulengeri and investigated its expression patterns among different growth temperatures and tissues. The complete open reading frame (ORF) sequence of Japonicin-pb precursor was amplified by RT-PCR according to the transcriptome database of Paa boulengeri constructed by our laboratory. The sequential structure of Japonicin-pb was analyzed by the method of domain alignment. The expression patterns of Japonicin-pb among different growth temperatures (15, 18, 21, 24, 27 and 30 ℃) and tissues (blood, muscle, liver and skin) were analyzed by real-time PCR. The results showed that according to the domain alignment, 3 Japonicin-pb precursors had the same N terminal signal peptide, middle spacer region, but the mature peptides were 19, 20 and 12 amino acid residues, named Japonicin-1apb, Japonicin-1bpb and Japonicin-3pb, respectively. The expression patterns showed that Japonicin-1apb was mainly expressed in skin, and its expression abundance increased gradually from 15 to 30 ℃; the expression level of Japonicin-1bpb among different tissues and growth temperatures were no significant difference; Japonicin-pb mainly distributed in liver, and it was not sensitive to the response of growth temperature. These results provide important clues for the functional study and the development of Japonicin-pb.

Cite this article

JIANG Yusong, CHEN Debi, ZOU Yong, ZHOU Jiping, FAN Wenqiao, XU Jingming . Isolation and Expression Patterns of Japonicin-pb from Paa boulengeri[J]. Chinese Journal of Animal Nutrition, 2015 , 27(5) : 1613 -1619 . DOI: 10.3969/j.issn.1006-267x.2015.05.034

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