The Research of Appropriate Sample Weight of the Method for Determining Non-Starch Polysaccharides of Feedstuffs Using Gas-Liquid Chromatography with Aiditol Acetates Derivatives

HUANG Qinghua, CHEN Liang, GAO Lixiang, LIU Qiang, LU Ling, TANG Xiangfang, LIU Lei, ZHANG Hongfu

doi:10.3969/j.issn.1006-267x.2015.05.035

2015 , Vol. 27 >Issue 5: 1620 - 1631

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.05.035

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The Research of Appropriate Sample Weight of the Method for Determining Non-Starch Polysaccharides of Feedstuffs Using Gas-Liquid Chromatography with Aiditol Acetates Derivatives

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China;
2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2014-11-30

Online published: 2015-05-13

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Abstract

The objectives of the present study was investigated appropriate sample weight and variation of the method for determining non-starch polysaccharides (NSP) of feedstuffs by gas chromatography with aiditol acetates derivatives to confirm the precision of assay and the variation of determined values. A single factorial completely randomized design with 7 sample weight treatments (0.10, 0.15, 0.20, 0.25, 0.30, 0.40 and 0.50 g) for reference dietary fiber (RDF) was adapted in Exp. 1 and each treatment contained 3 replicates. The contents of soluble and insoluble NSP of RDF was determined. In Exp. 2, the variation coefficients for determining NSP of RDF based on appropriate sample weight was analyzed in 2 batches with 3 replicates per batch. In Exp.3, the NSP of corn, soybean meal, wheat, wheat bran, cotton meal, rapeseed meal, sugar beet pulp was repeatedly determined based on appropriate sample weight. The results showed as follows: 1) total NSP of RDF with sample weight for 0.10, 0.15, 0.20 and 0.25 g (ranged from 21.33% to 21.88%, P>0.05) were significantly greater than for 0.30, 0.40 and 0.50 g (ranged from 18.06% to 19.32%, P<0.05). The insoluble NSP of RDF increased as the level of sample weight from 0.10 to 0.25 g, whereas the insoluble NSP of RDF significantly decreased by increasing the level of sample weight from 0.25 g to 0.50 g (linear, P<0.05). 2) The contents of monosaccharide, uronic acid, and total NSP were not significantly affected by the batch (P>0.05). The intra-batch, inter-batch and total coefficient of variation (CV) for soluble, insoluble and total NSP were less than 11.57%, 3.19% and 3.01%, respectively. 3) The content of total NSP for selective feed ingredients ranged from 8.59% to 65.59% and soluble NSP was less than the content of insoluble NSP. The primary monosaccharide components of feed ingredients were arabinose, xylose and glucose. The total NSP for cereal was greater than force real by-products. In conclusion, no more than approximate 55 mg NSP (the approximate content of NSP in 0.25 g RDF) of sample weight was used and the CV for total NSP are less than 3.01% in the present method using gas chromatography with aiditol acetates derivatives.

Key words： feedstuff; gas chromatography with aiditol acetates derivatives; non-starch polysaccharides; sample weight; coefficient of variation

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HUANG Qinghua, CHEN Liang, GAO Lixiang, LIU Qiang, LU Ling, TANG Xiangfang, LIU Lei, ZHANG Hongfu . The Research of Appropriate Sample Weight of the Method for Determining Non-Starch Polysaccharides of Feedstuffs Using Gas-Liquid Chromatography with Aiditol Acetates Derivatives[J]. Chinese Journal of Animal Nutrition, 2015 , 27(5) : 1620 -1631 . DOI: 10.3969/j.issn.1006-267x.2015.05.035

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