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Effects of Different Treated Rice Straws on Pattern, Methane Production and Microflora of in Vitro Ruminal Fermentation

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  • College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2014-12-23

  Online published: 2015-05-13

Abstract

The aim of this study was to investigate the effects of different treated rice straws on pattern, methane production and microflora of in vitro ruminal fermentation. Three Holstein cows fitted with permanent rumen fistulas were used as the donor of rumen fluid. Untreated dry rice straw (control), rice straw silage, aminated rice straws and alkalified rice straws were used as substrates, and the changes of gas production, methane production, ruminal fermentation parameters, as well as ruminal microbes during 72 h incubation were determined using in vitro gas production method. The results showed as follows: the treatments of silage, ammoniation and alkalization significantly increased 72 h gas production and 24 h total volatile fatty acid concentration of rice straws incubated in vitro (P<0.05), the treatment of alkalization significantly increased 72 h methane production (P<0.05), and the treatments of silage and ammoniation significantly increased 24 h propionate content (P<0.05) and significantly decreased acetate/propionate (P<0.05). Among all the treatments, gas production (220.3 mL) and methane production (43.23 mL) were the highest in the treatment of alkalization (P<0.05). Compared with untreated dried rice straws, the treatments of ammoniation and alkalization significantly increased the relative number of F. succinogenes in culture medium after 24 h in vitro incubation (P<0.05), and that of ammoniation significantly decreased the relative number of Methanogens (P<0.05), however, different treatments had no significant effects on the relative number of R.albus, R.flave faciens, protozoa and fungi (P>0.05). It is concluded that treatments of rice straw, such as silage, ammoniation and alkalization can change pattern, methane production and microflora of in vitro ruminal fermentation; considering from methane production control, the treatments of silage and ammoniation are optional.

Cite this article

XIN Hangshu, LIU Kaiyu, ZHANG Yonggen, WANG Mingjun, LI Zhongyu, WANG Zhibo, PAN Chunfang, LI Xinxin, LIU Keyuan . Effects of Different Treated Rice Straws on Pattern, Methane Production and Microflora of in Vitro Ruminal Fermentation[J]. Chinese Journal of Animal Nutrition, 2015 , 27(5) : 1632 -1640 . DOI: 10.3969/j.issn.1006-267x.2015.05.036

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