Simultaneous Measurement of Aflatoxin B1 and Zearalenone in Gastrointestinal Chyme of Pigs and Assessment of Binding Efficacy of Mycotoxin Adsorbents

AN Ya, WANG Danyang, DING Liren, YANG Xingang, DENG Yajun, LIU Qiang

doi:10.3969/j.issn.1006-267x.2015.06.019

2015 , Vol. 27 >Issue 6: 1823 - 1831

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.06.019

Feed Security

Simultaneous Measurement of Aflatoxin B₁ and Zearalenone in Gastrointestinal Chyme of Pigs and Assessment of Binding Efficacy of Mycotoxin Adsorbents

Expand

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2015-01-23

Online published: 2015-06-17

Fold

Abstract

This experiment was conducted to develop a high-performance liquid chromatographic (HPLC) method for quantitative determination of aflatoxin B₁ and zearalenone in supernatant liquor of gastric and intestinal of pigs, and the method was used to evaluate binding efficacy of aflatoxin B₁ and zearalenone by mycotoxin absorbents in different media. In order to eliminate the influence of complex composition in the supernatant, the experiment optimized extraction and purification of the supernatant, detection wavelengths and mobile phases of liquid chromatography. Binding efficacy of 4 mycotoxin adsorbents in a fixed mycotoxin concentration was evaluated respectively in purified water,the supernatant liquor of gastric and intestinal chyme of pigs. The results showed as follows: 1) the supernatant liquor samples were extracted with n-hexane and dichloromethane, the optimal chromatographic condition was that the fluorescent emitted light and excitation light wavelength were set as 360, 440 nm within 0 to 9 minutes and 235, 418 nm within 9 to 14 minutes, respectively, and the mobile phase was the mixture of acetonitrile and water with acetonitrile percentage in the range of 40% to 60%. The analytical method was quantitative determination for aflatoxin B₁ from 1.00 to 10 000.00 ng/mL and zearalenone from 20.00 to 5 000.00 ng/mL, respectively. The linear correlation coefficient of aflatoxin B₁ was 1; the limit of detection was 0.16 ng/mL, the intra-day and inter-day precision were 2.37% and 2.74%, with recoveries ranging from 89.1% to 100.3%. The linear correlation coefficient of zearalenone was 0.999; the limit of detection was 2.00 ng/mL, the intra-day and inter-day precision were 4.50% and 8.84%, with recovery ranging from 98.7% to 104.1%. 2) The adsorption rates of aflatoxin B₁ on P₃, M₃, Y₁ and Y₂ were 72.10%, 84.18%, 83.17% and 66.01% from gastric supernatant of pigs, and 78.15%, 88.08%, 88.12% and 67.34% from intestinal supernatant,respectively. The binding rate of zearalenone on P₃, M₃, Y₁ and Y₂ were 85.35%, 18.41% and 16.20% from gastric supernatant of pigs, and 12.98%, 39.75%, 25.15%, 21.40% and 24.85% from intestinal supernatant,respectively. In conclusion, the HPLC method is developed for simultaneous measurement of aflatoxin B₁ and zearalenone in supernatant liquor of gastric and intestinal chyme of pigs, and its detection limits and ranges meet the requirement of Hygienical Standard for Feeds in our country. The method is applied to evaluate binding efficacy of mycotoxin adsorbents under the condition of physiological body fluid of pigs.

Key words： aflatoxin B₁; zearalenone; mycotoxin adsorbent; supernatant liquor of chyme of pigs; binding efficacy

Cite this article

AN Ya, WANG Danyang, DING Liren, YANG Xingang, DENG Yajun, LIU Qiang . Simultaneous Measurement of Aflatoxin B₁ and Zearalenone in Gastrointestinal Chyme of Pigs and Assessment of Binding Efficacy of Mycotoxin Adsorbents[J]. Chinese Journal of Animal Nutrition, 2015 , 27(6) : 1823 -1831 . DOI: 10.3969/j.issn.1006-267x.2015.06.019

References

[1] Council for Agricultural Science and Technology.Mycotoxins:risks in plant,animal and human systems[M].Wisconsin:Council for Agricultural Science and Technology,2003:1-191.

[2] ZAIN M E.Impact of mycotoxins on humans and animals[J].Journal of Saudi Chemical Society,2011,15(2):129-144.

[3] DEVREESE M,DE BACKER P,CROUBEL S.Different methods to counteract mycotoxin production and its impact on animal health[J].Vlaams Diergeneeskundig Tijdschrift,2013,82(4):181-190.

[4] 齐德生.蒙脱石改性前后对AFB₁和营养成分的吸附及对AFB₁的脱毒效果[D].博士学位论文.武汉:华中农业大学,2003:75-85.

[5] YIANNIKOURIS A,ANDRÉ G,BULÉON A,et al.Comprehensive conformational study of key interactions involved in zearalenone complexation with β-D-glucans[J].Biomacromolecules,2004,5(6):2176-2185.

[6] MARROQUÍN-CARDONA A,DENG Y,TAYLOR J F,et al.In vitro and in vivo characterization of mycotoxin-binding additives used for animal feeds in Mexico[J].Food Additives and Contaminants,2009,26(5):733-743.

[7] PIMPUKDEE K,KUBENA L F,BAILEY C A,et al.Aflatoxin-induced toxicity and depletion of hepatic vitamin a in young broiler chicks:protection of chicks in the presence of low levels of Novasil Plus in the diet[J].Poultry Science,2004,83(5):737-744.

[8] PHILLIPS T D.Dietary clay in the chemoprevention of aflatoxin-induced disease[J].Toxicological Sciences,1999,52(Suppl.1):118-126.

[9] LEMKE S L,OTTINGER S E,MAYURA K,et al.Development of a multi-tiered approach to the in-vitro prescreening of clay-based enterosorbents[J].Animal Feed Science and Technology,2001,93(1):17-29.

[10] LEMKE S L,GRANT P G,PHILLIPS T D.Adsorption of zearalenone by organophilic montmorillonite clay[J].Journal of Agricultural and Food Chemistry,1998,46(9):3789-3796.

[11] AVANTAGGIATO G,HAVENAAR R,VISCONTI A.Evaluation of the intestinal absorption of deoxynivalenol and nivalenol by an in vitro gastrointestinal model,and the binding efficacy of activated carbon and other adsorbent materials[J].Food and Chemical Toxicology,2004,42(5):817-824.

[12] AVANTAGGIATO G,HAVENAAR R,VISCONTI A.Assessing the zearalenone-binding activity of adsorbent materials during passage through a dynamic in vitro gastrointestinal model[J].Food and Chemical Toxicology,2003,41(10):1283-1290.

[13] KOLOSOVA A,STROKA J.Evaluation of the effect of mycotoxin binders in animal feed on the analytical performance of standardised methods for the determination of mycotoxins in feed[J].Food Additives & Contaminants Part A:Chemistry Analysis Control Exposure & Risk Assessment,2009,29(12):1959-1971.

[14] ANKLAM E,STROKA J,BOENKE A.Acceptance of analytical methods for implementation of EU legislation with a focus on mycotoxins[J].Food Control,2002,13(3):173-183.

[15] KRSKA R,JOSEPHS R.The state-of-the-art in the analysis of estrogenic mycotoxins in cereals[J].Fresenius' Journal of Analytical Chemistry,2001,369(6):469-476.

[16] SPRYNSKYY M,GADZALA-KOPCIUCH R,NOWAK K,et al.Removal of zearalenone toxin from synthetics gastric and body fluids using talc and diatomite:a batch kinetic study[J].Colloids and Surfaces B:Biointerfaces,2012,94:7-14.

[17] STROKA J,HOLST C,ANKLAM E,et al.Immunoaffinity column cleanup with liquid chromatography using post-column bromination for determination of aflatoxin B₁ in cattle feed:collaborative study[J].Journal of AOAC International,2003,86(6):1179-1186.

[18] ARRANZ I,MISCHKE C,STROKA J,et al.Liquid chromatographic method for the quantification of zearalenone in baby food and animal feed:interlaboratory study[J].Journal of AOAC International,2007,90(6):1598-1609.

[19] 刘媛婷.霉菌毒素吸附剂对黄曲霉毒素B₁吸附特性及效果研究[D].硕士学位论文.雅安:四川农业大学,2011:38-43.

[20] MIAZZO R,ROSA C A R,CARVALHO E C D Q,et al.Efficacy of synthetic zeolite to reduce the toxicity of aflatoxin in broiler chicks[J].Poultry Science,2000,79(1):1-6.

[21] DÖLL S,DÄNICKE S,VALENTA H,et al.In vitro studies on the evaluation of mycotoxin detoxifying agents for their efficacy on deoxynivalenol and zearalenone[J].Archives of Animal Nutrition,2004,58(4):311-324.

[22] MAGNOLI A P,ALONSO V A,CAVAGLIERI L R,et al.Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B₁ adsorption ability by a montmorillonite[J].Food Additives & Contaminants:Part A,2013,30(4):743-749.

[23] MOSCHINI M,GALLO A,PIVA G,et al.The effects of rumen fluid on the in vitro aflatoxin binding capacity of different sequestering agents and in vivo release of the sequestered toxin[J].Animal Feed Science and Technology,2008,147(4):292-309.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References