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Effects of Chronic Immunological Stress Induced by Increasing Administration of Lipopolysaccharide on Growth Performance of Piglets

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  • 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
    3. College of Life Science, Anhui Agricultural University, Hefei 230036, China

Received date: 2015-01-23

  Online published: 2015-06-17

Abstract

This experiment was conducted to study the effects of gradually increasing 30% of lipopolysaccharide (LPS) by injecting on the chronic immunological stress and on growth performance and nutrient apparent digestibility of piglets. Sixteen weaned piglets were randomly allotted into 2 groups (control and LPS groups), and were individually housed in metabolic cages. Saline (control) or LPS were injected at 48 h intervals with an initial dosage of 80 μg/kg BW, increased by 30% at each following injections. The experiment lasted for 15 d. The results showed that the contents of interleukin-1β and cortisol in plasma of the LPS group were significantly higher than those of control group on days 1 and 5 (P<0.01), and there was a trend that the content of cortisol in plasma of LPS group was higher than that of control group (P=0.10). Moreover, the content of haptoglobin in plasma was significantly higher than that of control group during the whole experimental period (P<0.05). Compared with the control group, during three phases of this experiment (from 1 to 4 days, 5 to 8 days and 9 to 15 days), the average daily gain of LPS group was decreased by 40.00% (P<0.01), 29.52% (P<0.05) and 19.30% (P<0.05), respectively. For the average daily feed intake was decreased by 25.09% (P=0.01), 23.15% (P<0.05) and 19.47% (P<0.05), respectively. At last, no significant difference was found in gain to feed ratio (P>0.05). Compared with control group, the apparent digestibility of dry matter in LPS group was decreased by 5.43% (P<0.05), and gross energy apparent digestibility had a decreasing trend (P=0.06), apparent digestibility of crude protein and ether extract were decreased slightly (P>0.10). In conclusion, increasing of 30% injection of LPS successfully induces the chronic immunological stress, and reduces feed intake and nutrient apparent digestibility of piglet, as well as inhibites the growth of piglets.

Cite this article

JIA Anfeng, FENG Jinghai, ZHANG Minhong, LI Zeyang, CHANG Yu, XU Shengsheng, HE Chuan . Effects of Chronic Immunological Stress Induced by Increasing Administration of Lipopolysaccharide on Growth Performance of Piglets[J]. Chinese Journal of Animal Nutrition, 2015 , 27(6) : 1868 -1874 . DOI: 10.3969/j.issn.1006-267x.2015.06.024

References

[1] RAKHSHANDEH A,DE LANGE C F M.Evaluation of chronic immune system stimulation models in growing pigs[J].Animal,2012,6(2):305-310.  

[2] HOU Y Q,YAO K,WANG L,et al.Effects of α-ketoglutarate on energy status in the intestinal mucosa of weaned piglets chronically challenged with lipopolysaccharide[J].British Journal of Nutrition,2011,106(3):357-363.  

[3] ESCRIBANO D,CAMPOS P H R F,GUTIÉRREZ A M,et al.Effect of repeated administration of lipopolysaccharide on inflammatory and stress markers in saliva of growing pigs[J].The Veterinary Journal,2014,200(3):393-397.  

[4] CAMPOS P H R F,MERLOT E,DAMON M,et al.High ambient temperature alleviates the inflammatory response and growth depression in pigs challenged with Escherichia coli lipopolysaccharide[J].The Veterinary Journal,2014,200(3):404-409.  

[5] KELEY E B,SPEARS J W,AUMAN S K.Dietary phosphorus and an inflammatory challenge affect performance and immune function of weanling pigs[J].Journal of Animal Science,2001,79(2):413-419.

[6] LIU Y L ,LI D F,GONG L M,et al.Effects of fish oil supplementation on the performance and the immunological,adrenal,and somatotropic responses of weaned pigs after an Escherichia coli lipopolysaccharide challenge[J].Journal of Animal Science,2003,81(11):2758-2765.

[7] MAO X F,PIAO X S,LAI C H,et al.Effects of β-glucan obtained from the Chinese herb Astragalus membranaceus and lipopolysaccharide challenge on performance,immunological,adrenal,and somatotropic responses of weanling pigs[J].Journal of Animal Science,2005,83(10):2775-2782.

[8] KIM J C,MULLAN B P,FREY B,et al.Whole body protein deposition and plasma amino acid profiles in growing and/or finishing pigs fed increasing levels of sulfur amino acids with and without Escherichia coli lipopolysaccharide challenge[J].Journal of Animal Science,2012,90(Suppl.4):362-365.

[9] WEBEL D M,FINCK B N,BAKER D H,et al.Time course of increased plasma cytokines,cortisol,and urea nitrogen in pigs following intraperitoneal injection of lipopolysaccharide[J].Journal of Animal Science,1997,75(6):1514-1520.

[10] ASH S A,GRIFFIN G E.Effect of parenteral nutrition on protein turnover in endotoxaemic rats[J].Clinical Science,1989,76(6):659-666.

[11] DEITCH E A.Animal models of sepsis and shock:a review and lessons learned [J].Shock,1998,9(1):1-11.  

[12] WRIGHT K J,BALAJI R,HILL C M,et al.Integrated adrenal,somatotropic,and immune responses of growing pigs to treatment with lipopolysaccharide[J].Journal of Animal Science,2000,78(7):1892-1899.

[13] GAINES A M,CARROLL J A,YI G F,et al.Effect of menhaden fish oil supplementation and lipopolysaccharide exposure on nursery pigs Ⅱ.Effects on the immune axis when fed simple or complex diets containing no spray-dried plasma[J].Domestic Animal Endocrinology,2003,24(4):353-365.  

[14] DRITZ S S,OWEN K Q,GOODBAND R D,et al.Influence of lipopolysaccharide-induced immune challenge and diet complexity on growth performance and acute-phase protein production in segregated early-weaned pigs[J].Journal of Animal Science,1996,74(7):1620-1628.

[15] CARROLL J A,DANIEL J A,KEISLER D H,et al.Non-surgical catheterization of the jugular vein in young pigs[J].Laboratory Animals,1999,33(2):129-134.  

[16] 张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业出版社,2010.

[17] BESEDOVASKY H O,DEL R A.Immune-neuro-endocrine interactions:facts and hypotheses[J].Endocrine Reviews,1996,17(1):64-102.  

[18] KLASING K C,LAURIN D E,PENG R K,et al.Immunologically mediated growth depression in chicks:influence of feed intake,corticosterone and interleukin-1[J].Journal of Nutrition,1987,117(9):1629-1637.

[19] LI J,LI D F,XING J J,et al.Effects of β-glucan extracted from Saccharomyces cerevisiae on growth performance,and immunological and somatotropic responses of pigs challenged with Escherichia coli lipopolysaccharide[J].Journal of Animal Science,2006,84(9):2374-2381.  

[20] EICHER S D,MCKEE C A,CARROLL J A,et al.Supplemental vitamin C and yeast cell wall β-glucan as growth enhancers in newborn pigs and as immunomodulators after an endotoxin challenge after weaning[J].Journal of Animal Science,2006,84(9):2352-2360.  

[21] 张玉俊.糖皮质激素影响肉仔鸡采食量的机制研究[D].泰安:山东农业大学,2011.

[22] 冯焱.免疫应激对肉鸡消化系统、免疫功能及肠道微生物区系的影响[D].杨凌:西北农林科技大学,2012.

[23] 陈静,刘显军,张飞,等.谷氨酰胺对免疫应激仔猪肠道指数的影响研究[J].中国饲料,2010(4):34-40.

[24] BURKHOLDER K M,THOMPSON K L,EINSTEIN M E,et al.Influence of stressors on normal intestinal microbiota,intestinal morphology,and susceptibility to Salmonella enteritidis colonization in broilers[J].Poultry Science,2008,87(9):1734-1741.  

[25] Lobo S M,BACKER D D,SUN Q H,et al.Gut mucosal damage during endotoxic shock is due to mechanisms other than gut ischemia[J].Journal of Applied Physiology,2003,95(5):2047-2054.  
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