Effect of Estradiol Valerate on Lipid Metabolism of Female Bama Xiang-Pigs and Its Mechanism

JIANG Guoli, FAN Juexin, ZHANG Bin, LI Lili, YIN Yulong

doi:10.3969/j.issn.1006-267x.2015.06.026

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 6: 1883 - 1890

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.06.026

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Effect of Estradiol Valerate on Lipid Metabolism of Female Bama Xiang-Pigs and Its Mechanism

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1. Laboratory of Animal Nutrition and Human Health, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2015-01-22

Online published: 2015-06-17

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Abstract

To investigate the effect of estradiol valerate on lipid metabolism of female Bama Xiang-pigs and its mechanism, a total of 20 healthy female Bama Xiang-pigs of 35 days old were selected and randomly divided into two groups (control group and experimental group) with each group had 10 replicates and each replicate had 1 pig. The pigs in the control group were fed a basal diet, while the pigs in the experimental group were fed the basal diet supplemented with 0.66 mg/kg estradiol valerate (equivalent to 0.5 mg/kg estradiol) for 120 d. The results showed as follows: 1) diets supplemented with estradiol valerate significantly decreased the final body weight, body weight gain and body fat percentage of female Bama Xiang-pigs (P<0.05). 2) Diets supplemented with estradiol valerate significantly reduced the contents of glucose (GLU), triglyceride (TG) and low density lipoprotein (LDL) in blood of female Bama Xiang-pigs (P<0.05). 3) Compared with control group, the mRNA relative expression levels of fatty acid synthetase (FAS), acetyl CoA carboxylase (ACC) and lipoprteinlipase (LPL) in dorsal subcutaneous adipose of female Bama Xiang-pigs in experimental group were significantly decreased (P<0.05), and the mRNA relative expression levels of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL) and estrogen receptor-β (ER-β) were significantly increased (P<0.05), while the mRNA relative expression levels of peroxisome proliferators-activated receptor-γ (PPAR-γ), peroxisome proliferators-activated receptor-α (PPAR-α) and estrogen receptor-α (ER-α) were not significantly different (P>0.05); the mRNA relative expression levels of FAS, ACC and LPL in abdominal subcutaneous adipose were significantly decreased (P<0.05), and the mRNA relative expression levels of PPAR-α, HSL, ATGL, ER-α and ER-β were significantly increased (P<0.05), while the mRNA relative expression levels of PPAR-γ was not significantly different (P>0.05); the mRNA relative expression levels of PPAR-γ, FAS, ER-α and ER-β in perirenal adipose were significantly decreased (P<0.05), and the mRNA relative expression levels of HSL, ATGL and LPL were significantly increased (P<0.05), while the mRNA relative expression levels of ACC and PPAR-α were not significantly different (P>0.05). Those results indicate that estradiol valerate can control the body weight gain and fat deposition and improve the levels of blood GLU and TG of female Bama Xiang-pigs effectively. In addition, there may be exist two ways for estradiol valerate to regulate the lipid metabolism, one is that influencing the lipid metabolism in different white adipose tissues by regulating the expression of genes related to lipogenic and lipolytic metabolism, the other one is that modulating the expression of LPL by regulating the expression of estrogen receptors, then to affect the lipogenic metabolism.

Key words： female Bama Xiang-pig; estradiol valerate; body fat percentage; triglyceride; lipid metabolism; estrogen receptor

Cite this article

JIANG Guoli, FAN Juexin, ZHANG Bin, LI Lili, YIN Yulong . Effect of Estradiol Valerate on Lipid Metabolism of Female Bama Xiang-Pigs and Its Mechanism[J]. Chinese Journal of Animal Nutrition, 2015 , 27(6) : 1883 -1890 . DOI: 10.3969/j.issn.1006-267x.2015.06.026

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