The aim of this study was to determine the excretion patterns and carry-over rate of aflatoxin M1 (AFM1) in milk of dairy goats fed a single dose of aflatoxin B1 (AFB1). Three healthy Guanzhong dairy goats in mid-lactation with similar body weight were administered 1 mg of AFB1. Milk samples were collected after 0, 4, 8, 24, 32, 48, 56, 72, 80, 96, 104 and 120 h of AFB1 administration to determine AFM1 content. The results showed as follows: 1) AFM1 was not detected in milk before AFB1 administration (0 h), but was detected in the first milking following AFB1 administration (4 h); 2) the excretion pattern of AFM1 content in milk was very similar in all the experimental goats even if the values differed among animals; 3) The peak value for AFM1 content in milk was observed in milk collected at 4 and 8 h after AFB1 administration; 4) AFM1 average content in milk was 0.39 μg/kg at 56 h and 0.02 μg/kg at 120 h, which moved below the Chinese limit (0.5 μg/kg)and the European Union limit (0.05 μg/kg), respectively; 5) the maximum value of average carry-over rate of AFB1 into AFM1 in milk was 0.19% which appeared at 4 h; 6) the sum of average carry-over rate at different time point in 24 h was 81% of total average carry-over rate after AFB1 administration. In conclusion, AFM1 is mostly excreted in milk of dairy goats in 24 h after AFB1 administration.
WANG Lifang, YAO Yiping, LIAN Haifei, ZHANG Xinxin, LI Xiuping, ZENG Jie
. Excretion Pattern and Carry-Over Rate of Aflatoxin M1 in Milk of Dairy Goats after Fed Aflatoxin B1[J]. Chinese Journal of Animal Nutrition, 2015
, 27(6)
: 1915
-1920
.
DOI: 10.3969/j.issn.1006-267x.2015.06.030
[1] IARC.Some naturally occurring substances:food items and constituents,heterocyclic aromatic amines and mycotoxins[M].Lyon:World Health Organization,1993.
[2] COTTY P J,JAIME-GARCIA R.Influences of climate on aflatoxin producing fungi and aflatoxin contamination[J].Food Microbiol,2007,119:109-115.
[3] RICHARD J L.Some major mycotoxins and their mycotoxicoses-an overview[J].Food Microbiol,2007,119:3-10.
[4] Commission of The European Communities.European Commission Directive 2003/100/EC of 31 october 2003 amending annex Ⅰ to directive 2002/32/EC of the European Parliament and of the Council on undesirable substances in animal feed[J].European Journal of Communication,2003,285:33-37.
[5] U.S.FDA.CPG Sec.683.100 action levels for aflatoxins in animal feeds[EB/OL].[2015-03-20]http://www.fda.gov/ICECI/ComplianceManuals/CompliancePolicyGuidanceManual/ucm074703.htm.
[6] SMITH E E,PHILLIPS T D,ELLIS J A,et al.Dietary hydrated sodium calcium aluminosilicate reduction of aflatoxin M1 residue in dairy goat milk and effects on milk production and components[J].Journal of Experimental Animal Science,1994,72:677-682.
[7] VELDMAN A,MEIJS J A C, BORGGREVE G J,et al.Carryover of aflatoxin from cows' food to milk[J].Animal Production Science,1992,55:163-168.
[8] BATTACONE G,NUDDA A,CANNAS A,et al.Excretion of aflatoxin M1 in milk of dairy ewes treated with different doses of aflatoxin B1[J].Journal of Dairy Science,2003,86:2667-2675.
[9] BATTACONE G, NUDDA A,PALOMBA M,et al.Transfer of aflatoxin B1 from feed to milk and from milk to curd and whey in dairy sheep fed artificially contaminated concentrates[J].Journal of Dairy Science,2005,88:3063-3069.
[10] BATTACONE G,NUDDA A,PALOMBA M,et al.The transfer of aflatoxin M1 in milk of ewes fed diet naturally contaminated by aflatoxins and effect of inclusion of dried yeast culture in the diet[J].Journal of Dairy Science,2009,92:4997-5004.
[11] 金公亮.奶山羊饲养标准[J].畜牧兽医杂志,1989,8(2):7-12.
[12] BATTACONE G,NUDDA A,RASSU S P G,et al.Excretion pattern of aflatoxin M1 in milk of goats fed a single dose of aflatoxin B1[J].Journal of Dairy Science,2012,95:2656-2661.
[13] GALLO A, MOSCHINI M,MASOERO F.Aflatoxins absorption in the gastro-intestinal tract and in the vaginal mucosa in lactating dairy cows[J].Animal.Science,2008,7:53-63.
[14] COOK W O,RICHARD J L,OSWEILER G D,et al.Clinical and pathological changes in acute bovine aflatoxicoses:rumen motility and tissue and fluid concentrations of aflatoxin B1 and M1[J].American Journal of Veterinary Research,1986,47:1817-1825.
[15] HELFERICH W G,BALDWIN R L,HSIEH D P.[14C]-aflatoxin B1 metabolism in lactating goats and rats[J].Animal.Science,1986,62:697-705.
[16] NAGESWARA RAO S B,CHOPRA R C.Influence of sodium bentonite and activated charcoal on aflatoxin M1 excretion in milk of goats[J].Small Ruminant Research,2001,41:203-213.
