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Isolation, Screening and Identification of Tannin Degradable Bacteria in Rumen of Goats

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  • 1. Animal Science and Technology of Guangxi University, Nanning 530005, China;
    2. Nanning University, Nanning 530200, China

Received date: 2015-01-05

  Online published: 2015-06-17

Abstract

This study was conducted to isolate, screen and identify several strains of tannin degradable bacteria in rumen of goats. Ruminal contents was collected from goats, and were preliminary screened by tolerance culture medium with different tannic concentrations, then strains of tannin degradable bacteria were isolated, screened by tannase identification culture mediums. The screened strains were firstly identified by the methods of 16S rDNA gene sequencing, and then were cultured on tannase inductive culture mediums with different tannin concentrations (0.5%, 1% and 1.5%, respectively) for the analysis of enzyme activity. The results showed as follows: four strains of tannin degradable bacteria were isolated and screened. According to the results of 16S rDNA gene sequencing, the strains were Providencia alcalifaciens, Kerstersia, Providencia Vermicola and Pseudomonas aeruginosa, respectively. After cultured on tannase inductive culture medium, the intra-cellular tannase activity of Kerstersia was higher than that of the other three strains, which was pretty close to each other; after inducted by 1.5% tannin, the activity was significantly higher than that inducted by 0.5% and 1% tannin (P<0.05). In conclusion, four strains with high enzyme activity of tannin degradable bacteria in rumen of goats was successfully isolated, screened and identified.

Cite this article

LI Xingfan, XU Wen, ZHANG Shengnan, XU Gaoqiao, WANG Shanru, WANG Shichang, LIANG Shizhong . Isolation, Screening and Identification of Tannin Degradable Bacteria in Rumen of Goats[J]. Chinese Journal of Animal Nutrition, 2015 , 27(6) : 1921 -1927 . DOI: 10.3969/j.issn.1006-267x.2015.06.031

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