Short Communications

Effects of Salix Mixed Fermented Feed on Ruminal Environmental Parameters by in Vitro Gas Production Method

Expand
  • Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China

Received date: 2015-01-12

  Online published: 2015-06-17

Abstract

The current experiment was carried out to investigate the effects of Salix mixed fermented feed on ruminal environmental parameters by in vitro gas production method. According to the different proportion of Salix (36.44% and 67.08%), two groups of mixed fermentation feed were made, represented by FF1 and FF2 groups respectively. Each group was added the same number of compound bacteria preparation (live number ≥5×107 CFU/g). Gas production and gas production kinetic parameters in 96 h, and ruminal fermentation characteristic indexes in 24 h at different fermentation periods of 1, 3, 5, 7, 10, 12, 15, 22, 30, 45 and 60 days were determined, respectively. The results showed as follows: 1) with the extension of fermentation time, accumulative gas production in 96 h, digestible organic matter content and metabolizable energy were firstly decreased and then increased. 2) The concentrations of volatile fatty acids and microbial protein were significantly increased (P<0.05), while pH, the concentrations of ammonia nitrogen and butyric acid, and acetic acid/propionic acid were significantly decreased by Salix mixed fermentation feed (P<0.05). In conclusion, under in vitro culture condition, the two kinds of Salix mixed fermented feed with the supplemental proportion of Salix of 36.44% and 67.08% can effectively promote rumen microbial fermentation, raise energy utilization efficiency and increase the microbial protein synthesis in rumen, and the optimal supplemental proportion of Salix is 36.44% compared with 67.08%.

Cite this article

LI Manshuang, XUE Shuyuan, WANG Chao, WANG Limei, GUO Tianlong, JIN Hai . Effects of Salix Mixed Fermented Feed on Ruminal Environmental Parameters by in Vitro Gas Production Method[J]. Chinese Journal of Animal Nutrition, 2015 , 27(6) : 1943 -1953 . DOI: 10.3969/j.issn.1006-267x.2015.06.034

References

[1] 安保,白永祥,田志.沙柳生物学特性与造林技术研究[J].内蒙古林业科技,2003(Suppl.):24-26.

[2] 林瑄.重要的木本饲料树种-沙柳[J].饲料研究,1986(9):13-14.

[3] 李会科,邹厚远.榆林沙区主要人工固沙灌丛营养成分及对放牧羊只生育影响的研究[J].草业科学,2000,17(4):17-20.

[4] 薛树媛,李九月,金海,等.荒漠地区几种牧草和灌木中营养成分含量的动态变化[J].饲料工业,2011,32(1):44-47.

[5] 王晗生.黄土高原几种灌木的叶营养成分分析[J].西北林学院学报,1997,12(1):53-56.

[6] 李满双,薛树媛,郭天龙,等.贮藏时间对沙柳混合发酵饲料营养成分及体外消化率的影响[J].动物营养学报,2015,27(2):655-662.

[7] 熊本海,庞之洪,罗清尧.中国饲料成分及营养价值表(2013年第24版)制定说明[J].中国饲料,2013(21):33-33.

[8] MENKE K H,STEINGASS H.Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research Development,1988,28(1):7-55.

[9] ØRSKOV E R,MCDONALD I.The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage[J].Journal of Agricultural Science,1979,92(2):499-503.  

[10] MENKE K H,RAAB L,SALEWSKI A,et al.The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro[J].The Journal of Agricultural Science,1979,93(1):217-222.  

[11] 冯宗慈,高民.通过比色法测定瘤胃液氨氮含量方法的改进[J].内蒙古畜牧科学,1993,4: 40-41.

[12] 刘占英.绵羊瘤胃主要纤维降解细菌的分离鉴定及不同氮源对其纤维降解能力的影响[D].博士学位论文.呼和浩特:内蒙古农业大学,2006.

[13] 黄雅莉,邹彩霞,韦升菊,等.体外产气法研究半胱胺对水牛瘤胃发酵参数和甲烷产量的影响[J].动物营养学报,2014,26(1):125-133.

[14] 何亭漪.不同粗饲料在绵羊瘤胃和体外降解规律的研究及代谢能数学预测模型的建立[D].硕士学位论文.呼和浩特:内蒙古农业大学,2013.

[15] 程鹏辉,廖新俤,吴银宝.利用猪粪液为菌源体外发酵产气法评价牧草纤维品质[J].草业学报,2007,16(5):61-69.

[16] 胡明,卢德勋,牛文艺,等.不同阴阳离子水平日粮对绵羊瘤胃内环境参数的影响[J].内蒙古畜牧科学,2002(5):9-12.

[17] 王潍波,赵国琦,郭熠洁.体外条件下葡萄糖处理大豆粕对瘤胃内环境参数的影响[J].饲料工业,2009,30(17):34-37.

[18] 沈美英.日粮内不同粗饲料品质对绵羊瘤胃发酵功能和微生物区系的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2006.

[19] 王加启.反刍动物营养学研究方法[M].北京:现代教育出版社,2011.

[20] ALLISON M N,SMITH R H.Biosynthesis of aminoacids by ruminal microorganisms[J].Journal of Animal Science,1969,29(5):797-807.

[21] 李袁飞,郝建祥,马艳艳,等.体外瘤胃发酵法评定不同类型饲料的营养价值[J].动物营养学报,2013,25(10):2403-2413.

[22] 马慧,苗树君,刘君,等.反刍动物日粮正组合效应的调控及优化配制[J].黄牛杂志,2005,31(6):47-50.

[23] DIAZ A,AVENDANO M,ESCOBAR A.Evaluation of Sapindus saponaria as a defaunating agent and its effects on different rumen digestion parameters[J].Livestock Research for Rural Development,1993,5(2):1-6.

[24] SPEARS J W,SCHLEGEL P,SEAL M C,et al.Bioavailability of zinc from zinc sulfate and different organic zinc sources and their effects on ruminal volatile fatty acid proportions[J].Livestock Production Science,2004,90(2):211-217.

[25] 吕永艳,张杰杰,孙国强.不同比例的青贮玉米秸和花生蔓与精料的组合对奶牛体外瘤胃发酵参数的影响[J].中国畜牧杂志,2013,49(21):44-47.

[26] 管红娟. EAA构成与碳水化合物类型对混合瘤胃微生物体外发酵的影响[D].硕士学位论文.泰安:山东农业大学,2010.

[27] 丁健.不同结构日粮对肉用山羊瘤胃内环境参数及细菌氨态氮利用的影响[D].扬州:扬州大学,2003.

[28] 陈宁,尹君亮,李周权,等.日粮因素对反刍动物瘤胃消化代谢影响的研究现状[J].家畜生态学报,2006,27(3):106-108.
Outlines

/