Effects of Dietary Lipid Source and Emulsifier on Growth Performance, Intestinal Digestive Enzyme Activities and Hepatic Biochemical Indexes of Bullfrog, Rana (Lithobates) catesbeiana

FENG Wei, WANG Ling, ZHANG Chunxiao, SONG Kai, ZHANG Lu

doi:10.3969/j.issn.1006-267x.2015.06.035

2015 , Vol. 27 >Issue 6: 1954 - 1962

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.06.035

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Effects of Dietary Lipid Source and Emulsifier on Growth Performance, Intestinal Digestive Enzyme Activities and Hepatic Biochemical Indexes of Bullfrog, Rana (Lithobates) catesbeiana

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1. Key Laboratory of Healthy Mariculture for the East China Sea of Ministry of Agriculture, Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen 361021, China;
2. Tongwei Co., Ltd., Chengdu 610041, China

Received date: 2015-01-08

Online published: 2015-06-17

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Abstract

The objective of this study was to investigate the effects of using lard oil or fish oil as main lipid source supplemented with emulsifier on growth performance, intestinal digestive enzyme activities and hepatic biochemical indexes of bullfrog, Rana (Lithobates) catesbeiana. A randomized complete block design with 2×2 factors was adopted in the experiment. Four iso-nitrogenous and iso-energetic diets were formulated with dietary lipid source including pork lard and fish oil, and the supplemental level of emulsifier was 0 and 300 mg/kg, respectively. One hundred and eighty bullfrogs with the initial average body weight of (19.01±0.01) g were randomly assigned to 4 groups with 3 replicates per group and 15 bullfrogs per replicate. The experiment lasted for 8 weeks. The results showed that the weight gain rate (WGR) and feed intake (FI) of bullfrog fed with D3 (fish oil with 0 mg/kg emulsifier) diet were significantly higher than those fed with D1 (pork lard with 0 mg/kg emulsifier) diet (P<0.05), whereas, the WGR and FI of bullfrog fed with D2 (pork lard with 300 mg/kg emulsifier) diet were significantly higher than those fed with D4 (fish oil with 300 mg/kg emulsifier) diet (P<0.05). The WGR, specific growth rate (SGR) and FI of bullfrog fed with D2 diet were significantly higher than those fed with D1 diet (P<0.05), however, feed conversion ratio (FCR), nitrogen retention rate (NRE), energy retention rate (ERE) had no significant differences between two groups (P>0.05). The diet using fish oil as the main lipid source with or without emulsifier had no significant effects on all growth performance indexes (P>0.05). No significant difference was observed in all growth performance indexes between bullfrog fed with D3 and D4 diets (P>0.05). There were no significant differences in the contents of carcass moisture, crude protein and ash of bullfrog among all groups (P>0.05), however, whole-body crude lipid content of bullfrog fed with D1 diet was significantly lower than those fed with D2 diet (P<0.05). No significant differences in intestinal protease and amylase activities of bullfrog were observed among all groups (P>0.05), however, the supplementation of emulsifier in diets could significantly increase the lipase activity in intestine of bullfrog fed with diet using pork lard as main lipid source (P<0.05), but not significantly affect that of bullfrog fed with diet using fish oil as main lipid source (P>0.05). Hepatic catalase (CAT) and superoxide dismutase (SOD) activities had no significant difference among all the groups (P>0.05). When the diet using pork lard as main lipid source, the supplementation of emulsifier in the diet could significantly increase the hepatic malondialdehyde (MDA) content (P<0.05); when the diet using fish oil as main lipid source, the supplementation of emulsifier in the diet had no significant effect on the hepatic MDA content (P>0.05). These results indicate that the diet using lard oil as main lipid source supplemented emulsifier can improve the growth performance of bullfrog.

Key words： bullfrog, Rana (Lithobates) catesbeiana; lipid source; emulsifier; growth performance; body composition

Cite this article

FENG Wei, WANG Ling, ZHANG Chunxiao, SONG Kai, ZHANG Lu . Effects of Dietary Lipid Source and Emulsifier on Growth Performance, Intestinal Digestive Enzyme Activities and Hepatic Biochemical Indexes of Bullfrog, Rana (Lithobates) catesbeiana[J]. Chinese Journal of Animal Nutrition, 2015 , 27(6) : 1954 -1962 . DOI: 10.3969/j.issn.1006-267x.2015.06.035

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