Isolation and Identification of Anaerobic Cellulolytic Bacteria in Intestine of Rhizomyidae

CAO Xiaoyan, WANG Shangru, XU Wen, XU Gaoqiao, ZHANG Shengnan, WANG Shichang, LIANG Shizhong

doi:10.3969/j.issn.1006-267x.2015.06.038

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 6: 1976 - 1982

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.06.038

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Isolation and Identification of Anaerobic Cellulolytic Bacteria in Intestine of Rhizomyidae

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1. College of Animal Science and Technology of Guangxi University, Nanning 530005, China;
2. Nanning University, Nanning 530200, China

Received date: 2015-01-05

Online published: 2015-06-17

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Abstract

In order to isolate and identify anaerobic cellulolytic bacteria in intestine of rhizomyidae, cecum content of Rnizomyssinehsis was collected and diluted. Culture mediums with different kinds of cellulose [microcrystalline cellulose (MCC) and sodium carboxymethyl cellulose (CMC-Na), respectively] were used for preliminary screening of cellulolytic bacteria under anaerobic condition, and then multiple screening using culture medium with cellobiose as the only carbon source was carried out. The screened strains were identified by the methods of morphology and molecular biology. The results showed as follows: after preliminary screening and screening, two strains of anaerobic cellulolytic bacteria were isolated. After preliminary screening on culture medium with MCC and CMC-Na, the cellulolytic enzyme activity of final screened strains was 6.599 and 5.268 U/mL, respectively. After PCR identification and sequencing, the sequence was used to construct phylogenetic tree, and high homology was found between the two strains and Clostridium butyricum, which reached 99%. The two strains belonged to Clostridium. In conclusion, two strains of anaerobic cellulolytic bacteria are successfully screened from cecum in of rhizomyidae, and are belonged to Clostridium.

Key words： rhizomyidae; cecum; anaerobic; cellulolytic bacteria; 16S rDNA

Cite this article

CAO Xiaoyan, WANG Shangru, XU Wen, XU Gaoqiao, ZHANG Shengnan, WANG Shichang, LIANG Shizhong . Isolation and Identification of Anaerobic Cellulolytic Bacteria in Intestine of Rhizomyidae[J]. Chinese Journal of Animal Nutrition, 2015 , 27(6) : 1976 -1982 . DOI: 10.3969/j.issn.1006-267x.2015.06.038

References

[1] KANG M S,IM W T,JUNG H M,et al.Cellulomonas composti sp. nov. a cellulolytic bacterium isolated from cattle farm compost[J].International Journal of Systematic and Evolutionary Microbiology,2007,57(6):1256-1260.

[2] TRIPATHI V K,SEHGAL J P,PUNIYA A K,et al.Hydrolytic activities of anaerobic fungi from wild blue bull (Boselaphus tragocamelus)[J].Anaerobe,2007,13(1):36-39.

[3] THAREJA A,PUNIYA A K,GOEL G,et al.In vitro degradation of wheat straw by anaerobic fungi from small Ruminants [J].Archives of Animal Nutrition,2006,60(5):412-417.

[4] 何明清,程安春.动物微生物生态学[M].成都:四川科学技术出版社,2004:156-157.

[5] 刘占英,侯先志,刘玉承,等.一株分解纤维素的肠球菌的分离鉴定[J].内蒙古大学学报:自然科学版,2008,39(2):166-171.

[6] JOBLIN K N.Isolation,enumeration,and maintenance of rumen anaerobic fungi in roll tubes[J].Applied and Environmental Microbiology,1981,42(6):1119-1122.

[7] 张龙翔,张庭芳,李令嫒.生化实验方法与技术[M].2版.北京:高等教育出版社,1999.

[8] 张武先,王金华,熊智思,等.思茅松毛虫2龄幼虫肠道好氧细菌的筛选及毒力测定[J].安徽农业科学,2011,39(28):17288-17290.

[9] THOMPSON J D,GIBSON T J,PLEWNIAK F,et al.The CLUSTAL_X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools[J ].Nucleic Acids Research,1997,25(24):4876-4882.

[10] 罗辉,仇天雷,马诗淳,等.一株中温厌氧纤维素降解细菌的分离、鉴定及其系统发育分析[J].中国沼气 2008,26(3):3-7.

[11] PETITDEMANGE E,CAILLET F,GIALLO J,et al.Clostridium cellulolyticum sp. nov.,a cellulolytic,mesophilic:species from decayed grass[J].International Journal of Systematic and Evolutionary Microbiology,1984,34(2):155-159.

[12] MADDEN R H,BRYDER M J,POOLE N.Isolation and characterization of an anaerobic,cellulolytic bacterium,Clostridium papyrosolvens sp. nov.[J].International Journal of Systematic and Evolutionary Microbiology,1982,32(1):87-91.

[13] 刘亭婷,王晓霞.酪酸梭菌的作用机理及其在动物生产中的应用[J].中国饲料,2012(4):9-11.

[14] 蒲一涛,钟毅沪,周万龙.固氮菌和纤维素分解菌的混合培养及对生活垃圾降解的影响[J].环境科学与技术1999(1):15-18.

[15] 张晓昱,杜甫佑,王宏勋,等.不同木质纤维素基质上白腐菌降解特性的研究[J].微生物学杂志2004,24(6):4-7.

[16] 杨艳红,郑一敏,王伯初,等.多菌种固体共发酵生物软化稻壳的研究[J].微生物学通报,2004,31(4):14-18.

[17] 牛俊玲,崔宗均,李国学,等.高效纤维素降解菌复合系的筛选构建及其对秸秆的分解特性[J].农业环境科学学报,2005,24(4):795-799.

[18] 王伟东,崔宗均,牛俊玲,等.一组木质纤维素分解菌复合系的筛选及培养条件对分解活性的影响[J].中国农业大学学报,2004,5(9):7-11.

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