Molecular Nutrition

Effects of Lactoferrin on Growth Performance, Serum Antioxidant Indices and Tissue Antioxidant Gene Expressions of Diansa Weaned Piglets

  • AN Qingcong ,
  • LI Cenxi ,
  • ZHANG Chunyong ,
  • PAN Hongbin ,
  • LI Meiquan ,
  • XU Nana ,
  • CHEN Kelin ,
  • GUO Rongfu
Expand
  • Yunnan Key Laboratory of Animal Nutrition and Feeds, Yunnan Agricultural University, Kunming 650201, China

Received date: 2015-02-05

  Online published: 2015-07-07

Abstract

This experiment was conducted to investigate the effects of lactofferin (LF) on growth performance, serum antioxidant indices and tissue antioxidant gene expressions in Diansa weaned piglets. A total of 192 Diansa weaned piglets at the age of (28±2) days were randomly divided into 4 groups with 6 replicates in every group and with 8 piglets in every replicate. The control group was fed a basal diet, and LF1, LF2 and LF3 groups were respectively supplemented with 125, 250 and 500 mg/kg LF in the basal diet. Average daily weight (ADG), average daily feed intake (ADFI), feed to gain ratio (F/G) and diarrhea rate were determined after feeding 42 days. Kits were used for detecting serum antioxidant indices: total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD) activity, malondialdehyde (MDA) content and glutathione peroxidase (GSH-Px) activity. The expression levels of glutraredoxin 1 (GRX1), thioredoxin 1 (TRX1), superoxide dismutase (SOD) and GSH-Px genes in heart and liver were detected by real-time quantitative PCR (RT-qPCR). The results showed as follows: 1) compared with control group, LF2 and LF3 groups significantly improved ADG and ADFI (P < 0.05), LF1 and LF2 groups significantly (P < 0.05) reduced F/G, and LF2 and LF3 groups significantly reduced dairrhea rate (P < 0.05). 2) In serum, compared with control group, T-SOD activity were significantly increased in LF1 (P < 0.05), LF2 (P < 0.01) and LF3 (P < 0.05) groups, GSH-Px activity and T-AOC in LF2 group were significantly increased (P < 0.05), and the contents of MDA in LF2 and LF3 groups were significantly decreased (P < 0.05). 3) Compared with control group, the expression levels of GRX1, SOD and GSH-Px genes in heart and liver were increased in LF1 and LF2 groups. In liver, the difference of GSH-Px gene in LF1 group was significant (P < 0.05), and those of GRX1, SOD and GSH-Px genes in LF2 group were extremely significant (P < 0.01). TRX1 gene expression level in LF1 group was extremely significantly increased (P < 0.01). The expression levels of GRX1 and TRX1 genes in both tissues, SOD and GSH-Px genes in heart in LF3 group were decreased. and the differences of GRX1, TRX1 and SOD genes in heart was extremely significant (P < 0.01). The results suggest that the additon of LF (250 mg/kg was the best in this study) with a certain amount in diets can improve growth performance of weaned piglets, and enhance antioxidant capacity of piglets by improving the serum antioxidant indices and up-regulating antioxidant gene expressions.

Cite this article

AN Qingcong , LI Cenxi , ZHANG Chunyong , PAN Hongbin , LI Meiquan , XU Nana , CHEN Kelin , GUO Rongfu . Effects of Lactoferrin on Growth Performance, Serum Antioxidant Indices and Tissue Antioxidant Gene Expressions of Diansa Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2015 , 27(7) : 2018 -2026 . DOI: 10.3969/j.issn.1006-267x.2015.07.006

References

[1] HAWELL B.Biochemistry of oxidative stress[J].Biochemical Society Transactions,2007,35(5):1147-1150.  

[2] DURA?KOVÁ Z.Some current insights into oxidative stress[J].Physiological Research,2010,59(4):459-469.

[3] TOMITA M,WAKABAYASHI H,SHIN K,et al.Twenty-five years of research on bovine lactoferrin applications[J].Biochimie,2009,91(1):52-57.  

[4] EMBLETON N D,BERRINGTON J E,MCGUIRE W,et al.Lactoferrin:antimicrobial activity and t-herapeutic potential[J].Seminars in Fetal & Neonatal Medicine,2013,18(3):143-149.  

[5] 窦必成,赵玉香.滇撒猪配套系的饲养技术[J].中国畜牧兽医文摘,2011,27(1):49-50.

[6] 顾锡江,刘华戎,李发兰,等.滇撒猪配套系S1系生长肥育及胴体性能选育研究[J].现代农业科技,2014(9):276-277,279.

[7] COMSTOCK S S,REZNIKOV E A,CONTRACTOR N,et al.Dietary bovine lactoferrin alters mucosal and systemic immune cell responses in neonatal piglets[J].The Journal of Nutrition,2014,144(4):525-532.  

[8] OKAZAKI Y,KONO I,KURIKI T,et al.Bovine lactoferrin ameliorates ferric nitrilotriacetate-induced renal oxidative damage in rates[J].Journal of Clinical Biochemistry and Nutrition,2012,51(2):84-90.  

[9] PFAFFL M W.A new mathematical model for relative quantification in real-time RT-PCR[J].Nucleic Acids Research,2001,29(9):e45.

[10] WANG Y Z,SHAN T Z,XU Z R,et al.Effects of the lactoferrin (LF) on the growth performance,intestinal microflora and morphology of weanling pigs[J].Animal Feed Science and Technology,2007,135(3/4):263-272.

[11] 李美君,方成堃,张凯,等.饲粮中添加乳铁蛋白对早期断奶仔猪生长性能、肠道菌群及肠黏膜形态的影响[J].动物营养学报,2012,24(1):111-116.

[12] 伍喜林.乳铁蛋白对隔离早期断奶仔猪营养生理效应的研究[D].博士学位论文.雅安:四川农业大学,2004.

[13] 李婷,王彩云,闫序东,等.乳铁蛋白体外抗氧化性的研究[J].食品科学,2012,33(21):111-113.

[14] KRUZEL M L,ACTOR J K,RADAK Z,et al.Lactoferrin decreases LPS-induced mitochondrial dy-sfunction in cultured cells and in animal endotoxemia model[J].Innate Immunity,2010,16(2):67-79.  

[15] 张凯.乳铁蛋白对早期断奶仔猪生产性能的影响及其作用机理研究[D].硕士学位论文.长沙:湖南农业大学,2012:.25-27.

[16] 罗芳.牛初乳中乳铁蛋白分离纯化工艺及生物活性研究[D].硕士学位论文.长沙:湖南农业大学,2010:47-49.

[17] BAKER E N,BAKER H M.Molecular structure,binding properties and dynamics of lactoferrin[J].Cellular and Molecular Life Sciences,2005,62(22):2531-2539.  

[18] 徐奇友,单安山,王安.乳铁蛋白对早期断奶仔猪组织中微量元素含量的影响[J].动物营养学报,2005,17(4):62.

[19] 黄金昌,郭荣富.谷氧还蛋白和硫氧还蛋白对动物抗氧化应激生物学效应的研究进展[J].动物营养学报,2010,22(4):845-850.

[20] PILLAY C S,HOFMEYR J -H S,OLIVIER B G,et al.Enzymes or redox couples?The kinetics of thioredoxin and glutaredoxin reactions in a systems biology context[J].Biochemical Journal,2009,417(1):269-275.  

[21] GALLOGLY M M,SHELTON M D,QANUNGO S,et al.Glutaredoxin regulates apoptosis in cardiomyocytes via NFκB targets Bcl-2 and Bcl-xL:implications for cardiac aging[J].Antioxidants & Redox Signaling,2010,12(12):1339-1353.  

[22] 安清聪,张春勇,李美荃,等.谷氧还蛋白1和硫氧还蛋白1基因在高黎贡山猪不同组织中表达规律及维生素E对其在氧化应激细胞中表达的影响[J].动物营养学报,2013,25(8):1825-1835.

[23] XU C L,WANG Y Z,LIU J X,et al.Effect of dietary bovine lactoferrin on lipid peroxidation and activities,and mRNA levels of antioxidant enzymes of piglets[J].Journal of Animal and Feed Sciences,2006,15(4):609-620.
Outlines

/