Molecular Nutrition

Differentially Analysis of Liver Transcriptomes of Finishing Pigs Fed Different Dietary Fats Sources

  • LI Jianping ,
  • QIN Guixin ,
  • ZHAO Zhihui ,
  • ZHANG Qiaoling ,
  • WANG Dali ,
  • SUN Boxing ,
  • JIANG Hailong
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  • 1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;
    2. Department of Animal Husbandry, Henan University of Animal Husbandry and Economy, Zhengzhou 450011, China;
    3. Agriculture Test Station, Jilin University, Changchun 130062, China;
    4. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China

Received date: 2015-01-06

  Online published: 2015-07-07

Abstract

The study was to detect differentially expressed genes in liver of finishing pigs which were divided into two groups and separately fed safflower seed oil and coconut oil. The chosen pigs were slaughtered and liver tissue were isolated and their mRNA were respectively extracted. The liver transcriptomes were analyzed by Illumina HiSeq TM 2500 high-throughput RNA sequencing system. All RNA-Seq reads were mapped on the reference pig genome (Sscrofa10.2) using TopHat2 software. All of differentially expressed genes were annotated using Nr, GO and KEGG databases. The results showed that there were 938 differentially expressed genes in liver between two groups. 479 genes were up-regulated and 459 genes were down-regulated in safflower seed oil group vs. coconut oil group. There were 773 differentially expressed genes annotated into database of cellular component, 768 into biological process, 729 into molecular function in GO databases. There were 346 differentially expressed genes annotated into KEGG databases, and clustered pathways of differentially expressed genes included synthesis and degradation of ketone bodies and terpenoid backbone biosynthesis(P < 0.05).

Cite this article

LI Jianping , QIN Guixin , ZHAO Zhihui , ZHANG Qiaoling , WANG Dali , SUN Boxing , JIANG Hailong . Differentially Analysis of Liver Transcriptomes of Finishing Pigs Fed Different Dietary Fats Sources[J]. Chinese Journal of Animal Nutrition, 2015 , 27(7) : 2128 -2139 . DOI: 10.3969/j.issn.1006-267x.2015.07.018

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