Molecular Nutrition

Effects of Chlorogenic Acid on Apoptosis-Related Gene Expressions in Rats with Non-Alcoholic Fatty Liver Disease Caused by High-Fat Diet

  • LIU Yunlong ,
  • SONG Zhuo ,
  • PENG Bingjie ,
  • XU Shihao ,
  • ZHU Qi ,
  • WANG Zheng
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  • College of Bioscience & Biotechnology, Hunan Agriculture University, Changsha 410128, China

Received date: 2015-01-22

  Online published: 2015-07-07

Abstract

To investigate the effects of chlorogenic acid (CGA) on the expressions of apoptosis-related genes in rats with nonalcoholic fatty liver disease (NAFLD) caused by high-fat diet. Forty male SD rats were randomly divided into 5 groups: control group (NC group), high-fat diet model group (HFD group), high-fat diet+low dose CGA group [(20 mg/(kg·d), HFD-LC group], and high-fat diet+high dose CGA group [(90 mg/(kg·d), HFD-HC group]. Each group had 10 rats. All rats were weighed everyday and were killed after 12 weeks. Serum contents of alanine transarninase (ALT), total cholesterol (TC), triglyceride (TG), free fatty acid (FFA), high-density lipoprotein cholesterol (HDL-c) and low-density lipoprotein cholesterol (LDL-c) were detected; histological changes of the liver were examined by hematoxylin and eosin staining; liver wet weight and hepatic index were weighed; the mRNA expression levels of tumor necrosis factor-α (TNF-α), tumor necrosis factor receptor-1 (TNFR-1), caspase-8, caspase-3, B cell lymphoma/leukemia-2 (Bcl-2), Bcl-2 related gene (Bax), nuclear factor-κB (NF-κB), and interleukin 6 (IL-6) were assessed by semi-quantification RT-PCR. The results showed as follows: compared with NC group, final body weight (P > 0.05), liver wet weight (P < 0.01), hepatic index (P < 0.01), and the mRNA expression levels of apoptosis-related genes were much higher in HFD group. Compared with HFD group, final body weight (P > 0.05), liver wet weight (P < 0.05), hepatic index (P>0.05), and the mRNA expression levels of apoptosis-related genes were reduced after the treatment of low dose CGA; and high dose of CGA could induce more on those indices [final body weight (P<0.05), liver wet weight (P < 0.05), hepatic index (P>0.05)] than low dose CGA could do. The histological study revealed that there were a lot of fatty vacuoles, a large number of cells with apoptotic morphology. CGA dose dependently reduced the quantity of fatty vacuoles, and improve the physiological state of hepatic cells. It is concluded that CGA can reduce the chance of hepatic apoptosis caused by high-fat diet, improve fat metabolism, and has protection effects on rat liver.

Cite this article

LIU Yunlong , SONG Zhuo , PENG Bingjie , XU Shihao , ZHU Qi , WANG Zheng . Effects of Chlorogenic Acid on Apoptosis-Related Gene Expressions in Rats with Non-Alcoholic Fatty Liver Disease Caused by High-Fat Diet[J]. Chinese Journal of Animal Nutrition, 2015 , 27(7) : 2140 -2149 . DOI: 10.3969/j.issn.1006-267x.2015.07.019

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