Molecular Nutrition

Culture and Identification of the Chicken Intestinal Epithelial Cells Isolated from Chick Embryo in Vitro

  • ZHAN Kang ,
  • ZUO Xiaoxin ,
  • GONG Xiaoxiao ,
  • CHEN Yinyin ,
  • ZHAN Jinshun ,
  • ZHAO Guoqi
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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2015-02-01

  Online published: 2015-07-07

Abstract

This experiment was conducted to provide a stable model of chicken intestinal epithelial cell line for the study the pathogenesis of chicken intestinal diseases, chicken intestinal absorption of nutrients transport and immune mechanism. Our research applied the chicken intestinal tissue culture to successfully isolate and culture the chicken intestinal epithelial cell. We applied curettage, enzyme digestion, limiting dilution method to purify the chicken intestinal epithelial cell. MTT method was used to identify the growth curve of the chicken intestinal epithelial cell. The specific genes of chicken intestinal epithelial cells were detected by quantitative real-time PCR (RT-PCR). The results showed as follow: 1) the chicken intestinal epithelial cells could be successfully cultured by tissue culture cells. 2) The chicken intestinal epithelial cells started the proliferation 8 h after seeding the tissue. 3) The"S" shape of the growth curve of the chicken intestinal epithelial cells was accordance with the law of cell proliferation. 4) The chicken intestinal epithelial cells were swollen and started senescence and apoptosis after 5 passages. And many cells were not adherent to the bottom of the plate after seeded by passage. In conclusion, seeding the chicken intestinal tissue in 6-well plate, we are able to obtain a stable chicken intestinal epithelial cells line, which provides a stable cell model to study the pathogenesis of chicken intestinal disease, the absorption of chicken intestinal nutrients transport, immortalized chicken intestinal epithelial cells line.

Cite this article

ZHAN Kang , ZUO Xiaoxin , GONG Xiaoxiao , CHEN Yinyin , ZHAN Jinshun , ZHAO Guoqi . Culture and Identification of the Chicken Intestinal Epithelial Cells Isolated from Chick Embryo in Vitro[J]. Chinese Journal of Animal Nutrition, 2015 , 27(7) : 2150 -2156 . DOI: 10.3969/j.issn.1006-267x.2015.07.020

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