Identification of a Lactic Acid-Producing Strain of Bacillus sp. BLZ01 and Its Influence on Intestinal Flora of Broiler

ZHANG Dongmei; PAN Kangcheng; NI Xueqin; JING Bo; LIU Minggang

doi:10.3969/j.issn.1006-267x.2015.07.025

2015 , Vol. 27 >Issue 7: 2193 - 2200

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.07.025

Molecular Nutrition

Identification of a Lactic Acid-Producing Strain of Bacillus sp. BLZ01 and Its Influence on Intestinal Flora of Broiler

ZHANG Dongmei ,
PAN Kangcheng ,
NI Xueqin ,
JING Bo ,
LIU Minggang

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1. Research Center of Animal Microecology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Sichuan Province Key Laboratory of Animal Disease and Human Health, Chengdu 611130, China

Received date: 2015-01-30

Online published: 2015-07-07

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Abstract

The paper was conducted with the objective of identification of a lactic acid-producing strain of Bacillus sp. BLZ01 and its influence on the intestinal flora of the broiler. Combining the morphological, physiochemical properties with 16S rRNA sequencing analysis, strain of Bacillus sp. BLZ01 was identified. Twenty 7-day-old broilers were randomly divided into 2 groups (each group included 10 broilers). The control group was fed the basal diet. The experimental group was fed the basal diet supplemented with 0.05 g/kg of the BLZ01 probiotics. At the age of 42 days, the ileum and cecum microflora diversity of broilers were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed as follows:1) strain of BLZ01 was identified as Bacillus megaterium which could produce levolactic acid. 2) The number of PCR-DGGE bands and concentration of the ileum and cecum microflora in broilers treated with Bacillus megaterium BLZ01 was siginificantly increased compared with that of control group. 3) Lactobacillus sp. and uncultured bacteria were mainly appeared in the sequences of DNA recovered from single band in DGGE fingerprints by identified. The results indicated that strain of Bacillus BLZ01 is Bacillus megaterium which can produce levolactic acid; the abundance and population density of intestinal microflora in broiler treated with Bacillus megaterium BLZ01 are increased.

Key words： Bacillus megaterium; 16S rRNA; PCR-DGGE; intestinal microflora; broiler

Cite this article

ZHANG Dongmei , PAN Kangcheng , NI Xueqin , JING Bo , LIU Minggang . Identification of a Lactic Acid-Producing Strain of Bacillus sp. BLZ01 and Its Influence on Intestinal Flora of Broiler[J]. Chinese Journal of Animal Nutrition, 2015 , 27(7) : 2193 -2200 . DOI: 10.3969/j.issn.1006-267x.2015.07.025

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