Differences of Morphology and Casein Expression of Bovine Mammary Epithelial Cells under Different Culture Models

ZHAN Kang; ZUO Xiaoxin; GONG Xiaoxiao; CHEN Yinyin; ZHAN Jinshun; ZHAO Guoqi

doi:10.3969/j.issn.1006-267x.2015.07.031

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 7: 2241 - 2247

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.07.031

Molecular Nutrition

Differences of Morphology and Casein Expression of Bovine Mammary Epithelial Cells under Different Culture Models

ZHAN Kang ,
ZUO Xiaoxin ,
GONG Xiaoxiao ,
CHEN Yinyin ,
ZHAN Jinshun ,
ZHAO Guoqi

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College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2015-01-08

Online published: 2015-07-07

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Abstract

This research aimed to study the differences of morphology and casein expression of bovine mammary epithelial cells under two- and three-dimensional culture models. Bovine mammary epithelial cells were isolated by tissue mass culture, and were cloned by limiting dilution mehod. Morphology was observed in matrigel using microscopy. The induced casein expression was determined by Western blot method. The results showed as follows: 1) Bovine mammary epithelial cells could be successfully isolated by tissue mass culture, and the clones were passaged 20 generations stably. 2) After 12 h cultivation, the two-dimensional cultured bovine mammary epithelial cells neither formed apparent clusters nor produced interconnection, however, the three-dimensional cultured cells formed apparent clusters and produced interconnection under the action of laminin in matrigel. The bovine mammary epithelial cells in matrigel started to form network structure and produce interconnection. After 2 d of cultivation,the network structure among cells began to disappear, and single clusters started to appear, which made the connection among cells tighter. 3) The bovine mammary epithelial cells in two-dimensional culture did not express casein, but the expression of κ-casein could be induced in three-dimensional culture. It is concluded that the bovine mammary epithelial cells are able to change the morphology and function by three-dimensional culture, and can induce the κ-casein expression, can be used as an in vitro model for cows' lactating mechanism research.

Key words： bovine mammary epithelial cells; three-dimensional culture; casein; in vitro model

Cite this article

ZHAN Kang , ZUO Xiaoxin , GONG Xiaoxiao , CHEN Yinyin , ZHAN Jinshun , ZHAO Guoqi . Differences of Morphology and Casein Expression of Bovine Mammary Epithelial Cells under Different Culture Models[J]. Chinese Journal of Animal Nutrition, 2015 , 27(7) : 2241 -2247 . DOI: 10.3969/j.issn.1006-267x.2015.07.031

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