Molecular Nutrition

Effects of Low Protein Diet Supplemented with Tryptophan on Growth Performance, Body Composition, Immunity and Antioxidant Functions in Serum of Litopenaeus vannamei

  • QIU Jinmu ,
  • SUN Yuping ,
  • CAO Junming ,
  • HU Junru ,
  • WANG Guoxia ,
  • CHEN Bing ,
  • HUANG Yanhua
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  • 1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640 China;
    2. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;
    3. Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
    4. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Received date: 2015-01-29

  Online published: 2015-07-07

Abstract

This experiment was conducted to examine the effects of low protein diet supplemented with tryptophan on growth performance, body composition, immunity and antioxidant functions in serum of Litopenaeus vannamei. A total of 960 Litopenaeus vannamei with the average body weight of (2.00±0.01) g were randomly allocated into 6 groups (HT0, LT0, LT1, LT2, LT3 and LT4) with 4 replicates per group and 40 shrimp per replicate. Shrimp in HT0 were fed a high protein diet with 40.79% crude protein, and Shrimp in LT0, LT1, LT2, LT3 and LT4 were fed low protein diets which contained 37.01% crude protein and supplemented with 0, 1.20, 2.50, 5.00 and 10.00 g/kg tryptophan, respectively. The feeding experiment lasted for 8 weeks. The results showed that the final body weight, weight gain rate, specific growth rate in LT2 and LT3 were significantly higher than those in HT0 (P<0.05); the feed intake in LT1, LT2 and LT3 was significantly higher than that in HT0 and LT0 (P<0.05); the protein efficiency ratio in LT0, LT3 and LT4 was significantly higher than that in HT0 (P<0.05); the feed conversion rate was found no significant difference among HT0, LT0, LT3 and LT4 (P>0.05); the survival rate was lower in LT1 and LT3, but the other four groups showed no significant difference (P>0.05); the hepatosomatic index, moisture and ash contents of whole-body in LT1, LT2, LT3 and LT4 had no significant differences compared with HT0 and LT0 (P>0.05). There was no significant difference in the whole-body crude protein content among LT2, LT3, LT4 and HT0 (P>0.05), however, LT4 was significantly higher than LT0 and LT1 (P<0.05); the ether extract content of whole-body in LT1 and LT4 was significantly reduced compared with HT0 (P<0.05), while there no significant difference among LT0, LT2, LT3 and HT0 (P>0.05). The activities of phenol oxidase, alkaline phosphatase and total superoxide dismutase, and total antioxidant capacity in serum had no significant differences among all the groups (P>0.05); the serum catalase activity in LT2 was significantly higher than that in LT0 (P<0.05); the serum malonaldehyde content in LT2, LT3 and LT4 was significantly lower than that in LT0 (P<0.05), but it in LT2, LT3 and LT4 showed no significant difference compared with HT0 (P>0.05). In conclusion, tryptophan supplemented in the low protein diets can increase the whole-body protein deposition, improve the immunity and antioxidant functions, and enhance the growth performance. Regression analysis base on weight gain rate and serum catalase activity indicates that the optimal dietary tryptophan content in low protein diet (37.01% crude protein) for L. vannamei is 7.53 and 8.04 g/kg, respectively.

Cite this article

QIU Jinmu , SUN Yuping , CAO Junming , HU Junru , WANG Guoxia , CHEN Bing , HUANG Yanhua . Effects of Low Protein Diet Supplemented with Tryptophan on Growth Performance, Body Composition, Immunity and Antioxidant Functions in Serum of Litopenaeus vannamei[J]. Chinese Journal of Animal Nutrition, 2015 , 27(7) : 2272 -2281 . DOI: 10.3969/j.issn.1006-267x.2015.07.035

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