Effects of Pectin and Xylan on Growth Performance and Digestive Physiology of Chinese Mitten Crab (Eriocheir sinensis)

WU Tao; ZHANG Zhenlong; CAI Chunfang; YE Yuantu; LI Ting; ZHU Jianming

doi:10.3969/j.issn.1006-267x.2015.07.036

2015 , Vol. 27 >Issue 7: 2282 - 2291

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.07.036

Molecular Nutrition

Effects of Pectin and Xylan on Growth Performance and Digestive Physiology of Chinese Mitten Crab (Eriocheir sinensis)

WU Tao ,
ZHANG Zhenlong ,
CAI Chunfang ,
YE Yuantu ,
LI Ting ,
ZHU Jianming

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School of Biology & Basic Medical Sciences, Soochow University, Suzhou 215123, China

Received date: 2015-02-09

Online published: 2015-07-07

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Abstract

To reveal the potential anti-nutritional effects of non-starch polysaccharides (NSPs) in plant feed ingredients, the effects of pectin and xylan on growth performance and digestive physiology of Chinese mitten crab (Eriocheir sinensis) were studied. The pectin, enzymatic hydrolysis pectin, wheat bran (xylan source) and enzymatic hydrolysis wheat bran were supplied into the experimental diets at 8%, 8%, 30% and 30%, respectively. Chinese mitten crab with an initial body weight of (5.9±0.4) g were over-fed experimental diets for eight weeks. Each experimental diet had 3 replicates with 50 crabs. Chinese mitten crab with an initial body weight of (42.0±2.1) g were used to digestion rate experiments. Each experimental diet had 3 replicates with 15 crabs. The results showed that no significant difference in growth performance of Chinese mitten crab was detected among groups (P>0.05). The contents of serum cholesterol (CHO), high density lipoprotein (HDL) and low density lipoprotein (LDL) of crabs fed the diet containing pectin were significantly lower than that of crabs fed the diets containing enzymatic hydrolysis pectin, xylan and enzymatic hydrolysis xylan (P<0.05), and no significant differences were observed among the latter three groups (P>0.05). Hepatopancreas trypsin activity of crabs fed the diet containing pectin was significantly lower than that of crabs fed the diet containing enzymatic hydrolysis pectin (P<0.05); no significant difference was detected between crabs fed the diets containing xylan and enzymatic hydrolysis xylan (P>0.05), but both of them were significantly higher than of crabs fed the diet containing enzymatic hydrolysis pectin (P<0.05). Hepatopancreas amylase activity in xylan group was significantly lower than that in enzymatic hydrolysis xylan group (P<0.05). The apparent digestibility of dry matter, protein and lipid could be described as pectin grouP < enzymatic hydrolysis pectin grouP < xylan grouP< enzymatic hydrolysis xylan group. The apparent digestibility of dry matter and lipid in pectin group was significantly lower than that in enzymatic hydrolysis pectin (P<0.05), and the apparent digestibility of protein and lipid in xylan group was significantly lower than that in enzymatic hydrolysis xylan group (P<0.05). The intestinal mucosa of crab was covered by peritrophic membrane (PE), and no difference was observed in the thichness of PE, the width or height of fold among groups. In conclusion, pectin and xylan can reduce nutrient apparent digestibility, but no impact on intestinal morphology of Chinese mitten crab; pectin and xylan show different intensity of anti-nutritional effects on Chinese mitten crab, and on the whole they demonst pectin higher than xylan.

Key words： Chinese mitten crab (Eriocheir sinensis); pectin; xylan; digestive function; growth performance

Cite this article

WU Tao , ZHANG Zhenlong , CAI Chunfang , YE Yuantu , LI Ting , ZHU Jianming . Effects of Pectin and Xylan on Growth Performance and Digestive Physiology of Chinese Mitten Crab (Eriocheir sinensis)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(7) : 2282 -2291 . DOI: 10.3969/j.issn.1006-267x.2015.07.036

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