Effects of High Lipid Diet on Cecal Microbial Community Structure of Japanese Quail (Cotur nix japonica)

LIU Shasha; ZHANG Hongfu; ZHEMG Mingde; JI Cheng

doi:10.3969/j.issn.1006-267x.2015.08.008

Chinese Journal of Animal Nutrition >

0 2368 - 2377

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.08.008

Molecular Nutrition

Effects of High Lipid Diet on Cecal Microbial Community Structure of Japanese Quail (Cotur nix japonica)

LIU Shasha ,
ZHANG Hongfu ,
ZHEMG Mingde ,
JI Cheng

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science, Chinese Agruculture University, Beijing 100193, China;
3. Avian Research Centre, Faculty of Land and Food Systems, The University of British Columbia, Vancouver V6T1Z4, Canada

Received date: 2015-02-16

Online published: 2019-01-01

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Abstract

The object of this study was to investigate the effects of high lipid diet on cecal microbial community structure and composition and its lipid metabolism of Japanese quail (Cotur nix japonica). A total of 160 one-day-old Japanese quail fed a semi-synthetic diet for the first 6 weeks. At 6 weeks of age, the birds were randomly divided into 2 groups with 8 replicates per group and 10 birds per replicate, fed either a regular synthetic diet or a synthetic diet with added cholesterol for another 6 weeks. After the feeding trial, 8 birds from each group were euthanized and collected blood for testing the lipid metabolism indices, cecal contents for investigating the microbial community by using pyrosequencing.The results showed as follows: 1) The content of total cholesterol (TC), low density lipoprotein (LDL) and LDL/high density lipoprotein (HDL) of Japanese quail were significantly increased in high lipid diet group (P<0.05). 2) High lipid diet significantly decreased cecal microbial diversity (P<0.05); in high lipid diet group, the abundance of family Lactobacillales, Streptococcaceae and Clostridiales, others was significantly decreased (P<0.05), while the abundance of family Erysipelotrichales, Erysipelotrichaceae was significantly increased (P<0.05). 3) The abundance of cc_115 (Erysipelotrichaceae) and unclassified bacteria belong to Lachnospiraceae were positively correlated with contents of TC, LDL and LDL/HDL (P<0.05), besides Eubacterium (Erysipelotrichaceae) was only positively correlated with LDL/HDL (P<0.05), while the abundance of Ruminococcus (Ruminococcaceae) was negatively correlated with contents of TC, LDL and LDL/HDL (P<0.05), and the abundance of Ruminococcus(Lachnospiraceae) was positively correlated with contents of HDL (P<0.05). In conclusion, high lipid diet highly affects the cecal microbial community structure and composition and its lipid metabolism of Japanese quail.

Key words： high lipid diet; cecal microbial community; lipid metabolism

Cite this article

LIU Shasha , ZHANG Hongfu , ZHEMG Mingde , JI Cheng . Effects of High Lipid Diet on Cecal Microbial Community Structure of Japanese Quail (Cotur nix japonica)[J]. Chinese Journal of Animal Nutrition, 0 : 2368 -2377 . DOI: 10.3969/j.issn.1006-267x.2015.08.008

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