Effects of Dietary Supplementation of L-Tryptophan on Performance, Egg Quality and Serum Biochemical Parameters of Xinyang Green Shell Laying Hens

HE Qiang; DING Yang; YUAN Chao; LI Junming; YAN Huaxiang; ZOU Xiaoting

doi:10.3969/j.issn.1006-267x.2015.08.010

2015 , Vol. 27 >Issue 8: 2388 - 2395

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.08.010

Molecular Nutrition

Effects of Dietary Supplementation of L-Tryptophan on Performance, Egg Quality and Serum Biochemical Parameters of Xinyang Green Shell Laying Hens

HE Qiang ,
DING Yang ,
YUAN Chao ,
LI Junming ,
YAN Huaxiang ,
ZOU Xiaoting

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1. Key laboratory of Animal Nutrition and Feed Science of Agriculture in East China, Feed Science Institute, Zhejiang University, Hangzhou 310058, China;
2. Shanghai Poultry Breeding Co., Ltd., Shanghai Academy of Agriculture Sciences, Shanghai 201106, China

Received date: 2015-03-16

Online published: 2015-08-13

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Abstract

This study was conducted to investigate the effects of dietary supplementation of L-tryptophan (L-Trp) on performance, egg quality and serum biochemical parameters of Xinyang Green Shell laying hens, and to obtain the optimal supplemental levels of L-Trp for green shell laying hens (29 to 40 weeks of age). A total of 525 Xinyang green shell laying hens (28 weeks of age) with the similar egg production were randomly allotted to 5 treatments with 5 replicates of 21 birds each. The control treatment was given a basal diet containing 0.15% L-Trp, and the experiment treatments were supplemented with 200, 400, 600 and 800 mg/kg of L-Trp, to obtain the L-Trp levels in the experimental diets were 0.17%, 0.19%, 0.21% and 0.23%, respectively. The feeding trial lasted 13 weeks, including a 1-week acclimation period and a 12-week experimental period. The results were shown as follows: 1) dietary L-Trp significantly increased egg production and average egg weight (P<0.05), and decreased feed/egg (P<0.05), while no significant effect in feed intake (P>0.05) was found. 2) Albumen height and Haugh unit increased as dietary L-Trp increased from 0.15% to 0.19 and then decreased as L-Trp increased from 0.19% to 0.23%. Compared with the control group, albumen height and Haugh unit in 0.19% L-Trp group were significantly increased by 1.09% (P<0.05) and 6.5% (P<0.05), respectively. 3) Dietary L-Trp significantly increased serum total protein content (P<0.05) and decreased serum urea nitrogen content (P<0.05), but no significant effects on serum albumin and serum uric acid contents (P>0.05);dietary L-Trp significantly decreased serum free fatty acid content (P<0.05) and increased serum very low density lipoprotein content (P<0.05), while supplementing L-Trp had no significant effects on serum triglyceride, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol contents (P>0.05). In conclusion, the optimal tryptophan level of Xinyang green shell laying hens in peak period is 0.21% to 0.23% in this study.

Key words： tryptophan; green shell laying hens; performance; egg quality; serum biochemical parameters

Cite this article

HE Qiang , DING Yang , YUAN Chao , LI Junming , YAN Huaxiang , ZOU Xiaoting . Effects of Dietary Supplementation of L-Tryptophan on Performance, Egg Quality and Serum Biochemical Parameters of Xinyang Green Shell Laying Hens[J]. Chinese Journal of Animal Nutrition, 2015 , 27(8) : 2388 -2395 . DOI: 10.3969/j.issn.1006-267x.2015.08.010

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