Molecular Nutrition

Effects of Niacin on Ruminal Fermentation Indexes and Microflora of Water Buffaloes

  • LIN Bo ,
  • LIANG Xin ,
  • WEI Shengju ,
  • LI Lili ,
  • LIANG Xianwei ,
  • LI Ping ,
  • ZOU Caixia
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  • Key Laboratory of Buffalo Genetics, Breeding and Reproduction, Ministry of Agriculture and Guangxi, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China

Received date: 2015-03-02

  Online published: 2015-08-13

Abstract

This study was conducted to study the effects of dietary addition of niacin on ruminal fermentation indexes and microflora of water buffaloes. Thirty-six healthy hybrid water buffaloes with similar body weight were selected and divided into four groups randomly. The four groups were fed the same basal diet but added with 0 (control), 4, 8 and 12 g/d, respectively. Niacin was mixed in concentrate to feed. The advanced experiment feeding period was 14 days and the formal experimental period was 42 days. At the end of experiment, rumen fluid samples were taken from rumen by stomach tube to test the ruminal fermentation indexes, 16S rRNA genes of ruminal bacteria and methanogen were tested to determine microbial community by Illumina Miseq PE250 high-throughput sequencing. The results showed as follows: 1) compared with control group, the addition of 12 g/d of niacin increased average daily feed intake (P<0.05) and rumen fluid ammonia concentration (P>0.05), niacin groups significantly decreased rumen fluid total volatile fatty acid (TVFA) concentration (P<0.05), but the rumen fluid pH was not significantly influenced (P>0.05). 2) Water buffalo ruminal bacterial community was dominated by Bacteroidetes and Firmicutes at phyla level, Prevotellaceae and Fibrobacteraceae at family level, while ruminal methanogen community was dominated by Methanobrevibacter (over 90%) at genus level, followed by Methanosphaera and Thermoplasma. Water buffalo ruminal bacteria and methanogen community composition was generally similar with other ruminants which could utilize plant fiber. 3) Compared with control group, the addition of niacin significantly decreased ruminal Firmicutes the proportion (P<0.05), and significantly increased ruminal Fibrobacteraceae proportion (P<0.05), but there was no influence on methanogen community (P>0.05). In conclusion, the addition of niacin changes ruminal fermentation indexes and microflora of water buffaloes, the changes of ruminal fermentation indexes on one hand are related with release on heat stress and improvement on digestion of niacin, on another hand are related with the change of ruminal microflora.

Cite this article

LIN Bo , LIANG Xin , WEI Shengju , LI Lili , LIANG Xianwei , LI Ping , ZOU Caixia . Effects of Niacin on Ruminal Fermentation Indexes and Microflora of Water Buffaloes[J]. Chinese Journal of Animal Nutrition, 2015 , 27(8) : 2396 -2404 . DOI: 10.3969/j.issn.1006-267x.2015.08.011

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