Effects of Glucose Supplemental Level at Different Protein Levels on Growth Performance, Hepatic Glycolysis and Gluconeogenic Key Enzyme Activities of Large Yellow Croaker (Larmichthys crocea Richardson)

WANG Mengqiang; ZHOU Piaoping; HUANG Wenwen; ZHOU Qicun

doi:10.3969/j.issn.1006-267x.2015.08.015

2015 , Vol. 27 >Issue 8: 2431 - 2442

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.08.015

Molecular Nutrition

Effects of Glucose Supplemental Level at Different Protein Levels on Growth Performance, Hepatic Glycolysis and Gluconeogenic Key Enzyme Activities of Large Yellow Croaker (Larmichthys crocea Richardson)

WANG Mengqiang ,
ZHOU Piaoping ,
HUANG Wenwen ,
ZHOU Qicun

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School of Marine Sciences, Ningbo 315211, China

Received date: 2015-03-16

Online published: 2015-08-13

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Abstract

An 8-week feeding trial was conducted to evaluate the effects of glucose supplemental level at different protein levels on growth performance, serum indices, hepatic glycolysis and gluconeogenic key enzyme activities, glycogen content and digestive enzyme activities of large yellow croaker (Larmichthys crocea Richardson). Six experimental diets were formulated to contain two protein levels (42% and 48%, respectively) and three glucose supplemental levels (10%, 20% and 30%, respectively). Each diet was randomly assigned to 3 replicates, and each replicate had 50 juvenile large yellow croaker with the average body weight of (14.89±0.11) g. The results showed that weight gain rate (WGR) and specific growth rate (SGR) were significantly affected by the interaction of dietary protein level and glucose supplemental level (P<0.05). When the dietary protein level was 42%, the WGR and SGR showed a increasing trend with the glucose supplemental level increasing; however, when the dietary protein was 48%, fish fed the diet supplemented with 10% glucose had higher WGR and SGR than fish fed the diet supplemented with 30% glucose (P>0.05). The interaction of dietary protein level and glucose supplemental level had a significant effects on the ativities of glucokinase (GK), phosphofructokinase (PFK), pyruvate kinase (PK), glucose-6-phosphatase (G6Pase), fructose-1,6-bisphosphatase (FBPase), phosphoenolpyruvate (PEPCK) in liver (P<0.05). When the dietary protein level was 42%, the activities of GK, PFK, PK, G6Pase, FBPase and PEPCK in liver were significantly affected by dietary glucose supplemental level (P<0.05), and the GK activity was significantly increased with the glucose supplemental level increasing (P<0.05), while the PEPCK activity had a decreasing trend with the glucose supplemental level increasing. When the dietary protein level was 48%, the activities of GK, G6Pase and FBPase in liver were significantly affected by dietary glucose supplemental level (P<0.05), and the GK activity had a increasing trend with the glucose supplemental level increasing. The interaction of dietary protein level and glucose supplemental level had a significant effect on hepatic glycogen content (P<0.05), and the hepatic glycogen content had a increasing trend with the glucose supplemental level increasing when dietary protein level was 42% or 48%. The interaction of dietary protein level and glucose supplemental level only significantly affected a few serum indices (such as total protein and glucose contents, and alanine aminotransferase and aspartate aminotransferase activities in serum) (P<0.05), and did not significantly affected pepsin, intestinal amylase and lipase activities (P>0.05). The results indicated that, when the dietary glucose level is 42%, large yellow croaker has the ability to maintain blood glucose content by adjusting the hepatic glycogen, glycolysis and gluconeogenic key enzymes activities with the glucose supplemental level increasing; while, when the dietary glucose level is 48%, the ability of glucose utilization is decreased with the glucose supplemental level increasing.

Key words： large yellow croaker; protein; glucose; glycolysis key enzymes; gluconeogenic key enzymes

Cite this article

WANG Mengqiang , ZHOU Piaoping , HUANG Wenwen , ZHOU Qicun . Effects of Glucose Supplemental Level at Different Protein Levels on Growth Performance, Hepatic Glycolysis and Gluconeogenic Key Enzyme Activities of Large Yellow Croaker (Larmichthys crocea Richardson)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(8) : 2431 -2442 . DOI: 10.3969/j.issn.1006-267x.2015.08.015

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