Effects of Isobutyrate on Gene Expressions of Ruminal and Small Intestinal Mucosa of Calves

LIANG Yan; LIU Qiang; WANG Cong; ZHANG Yanli; PEI Caixia; WANG Yongxin; GUO Gang; HUO Wenjie; ZHANG Shuanlin; SHI Caiye; LIU Jianxin

doi:10.3969/j.issn.1006-267x.2015.08.021

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 8: 2483 - 2492

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.08.021

Molecular Nutrition

Effects of Isobutyrate on Gene Expressions of Ruminal and Small Intestinal Mucosa of Calves

LIANG Yan ,
LIU Qiang ,
WANG Cong ,
ZHANG Yanli ,
PEI Caixia ,
WANG Yongxin ,
GUO Gang ,
HUO Wenjie ,
ZHANG Shuanlin ,
SHI Caiye ,
LIU Jianxin

Expand

1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
2. College of Animal Science, Zhejiang University, Hangzhou 310058, China

Received date: 2015-03-23

Online published: 2015-08-13

Fold

Abstract

This study was conducted to examine the effects of dietary isobutyrate supplementation on gene expressions related to rumen and small intestine development of pre-weaning and post-weaning calves. Thirty-six 30-day-old Chinese Hostein dairy calves with similar body weight [(40.0±0.2) kg] were divided into four groups at random. Isobutyrate was added to a basal diet with 0 (control), 3, 6 and 9 g/d, respectively. Three calves from each group were slaughtered at the age of 45, 60 (weaning age) and 90 days, samples of ruminal and small intestinal mucosa were collected to detect the mRNA expression levels of growth hormone receptor (GHR), insulin receptor (INSR), 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1) and sodium-glucose cotransporter 1 (SGLT1) by the real-time quantitative PCR. The results showed that body weight and mRNA expression levels of GHR, INSR and HMGCS1 increased significantly with age (P<0.05), but SGLT1 mRNA expression level firstly decreased then increased before and after weaning, and the changes were significant (P<0.05). At the age of 45 and 60 days, body weight in 9 g/d isobutyrate group was significantly higher than that in control group (P<0.05), but was not significant different from that in 6 g/d isobutyrate group (P>0.05). At all days of age and all parts of rumen and small intestine, the mRNA expression levels of GHR, INSR and HMGCS1 in 6 and 9 g/d isobutyrate groups were higher than those in control group, and there were no significant differences between the two groups (P>0.05). At all parts of small intestine, the supplementation of isobutyrate did not affect the mRNA expression level of SGLT1 of 45-day-old and 90-day-old calves (P>0.05). At the age of 60 days, mRNA expression level of SGLT1 of duodenum in 6 and 9 g/d isobutyrate groups was significantly higher than that in control group and 3 g/d isobutyrate group, while mRNA expression level of SGLT1 of proximal jejunum, distal jejunum and ileum in 9 g/d isobutyrate group was significantly higher than that in control group (P<0.05), but had no significant difference with that in 6 g/d isobutyrate group (P>0.05). The results indicate that the growth of calves and the development of ruminal and small intestinal mucosa can be promoted by the supplementation of isobutyrate, the optimal dose of isobutyrate is 6 g/d under conditions in the present study.

Key words： isobutyrate; calves; ruminal mucosa; samll intestinal mucosa; gene expression

Cite this article

LIANG Yan , LIU Qiang , WANG Cong , ZHANG Yanli , PEI Caixia , WANG Yongxin , GUO Gang , HUO Wenjie , ZHANG Shuanlin , SHI Caiye , LIU Jianxin . Effects of Isobutyrate on Gene Expressions of Ruminal and Small Intestinal Mucosa of Calves[J]. Chinese Journal of Animal Nutrition, 2015 , 27(8) : 2483 -2492 . DOI: 10.3969/j.issn.1006-267x.2015.08.021

References

[1] 冯仰廉.反刍动物营养学[M].北京:科学出版社,2004.

[2] 王仁杰.异位酸对南江黄羊体外瘤胃发酵、生产性能及血液指标的影响[D].硕士学位论文.雅安:四川农业大学,2012.

[3] 沈冰蕾,苗树君,邵广.不同种类及水平的异位酸对奶牛日粮消化率及瘤胃纤维素酶活性的影响[J].中国畜牧杂志,2013,49(5):39-43.

[4] WANG A,JIANG H.Rumen fluid inhibits proliferation and stimulates expression of cyclin-dependent kinase inhibitors 1A and 2A in bovine rumen epithelial cells[J].Journal of Animal Science,2010,88(10):3226-3232.

[5] BALDWIN R L.The proliferative actions of insulin,insulin-like growth factor-Ⅰ,epidermal growth factor,butyrate and propionate on ruminal epithelial cells in vitro[J].Small Ruminant Research,1999,32(3):261-268.

[6] HEINRICHS A J,LESMEISTER K E.Rumen development in the dairy calf[J].Advances in Dairy Technology,2005,17:179-187.

[7] DROZDOWSKI L A,DIXON W T,MCBURNEY M I,et al.Short-chain fatty acids and total parenteral nutrition affect intestinal gene expression[J].Journal of Parenteral & Enteral Nutrition,2002,26(3):145-150.

[8] KATO S I,SATO K,CHIDA H,et al.Effects of Na-butyrate supplementation in milk formula on plasma concentrations of GH and insulin,and on rumen papilla development in calves[J].Journal of Endocrinology,2011,211(3):241-248.

[9] GUIMARAES K C,HAZELTON S R,MATTHEWS J C,et al.Influence of starch and casein administered postruminally on small intestinal sodium-glucose cotransport activity and expression[J].Brazilian Archives of Biology and Technology,2007,50(6):963-970.

[10] 刘强,黄应祥,王聪,等.异戊酸对西门塔尔牛瘤胃发酵及尿嘌呤衍生物的影响[J].畜牧兽医学报,2007,38(2):155-160.

[11] LIU Q,WANG C,YANG W Z,et al.Effects of isobutyrate on rumen fermentation,lactation performance and plasma characteristics in dairy cows[J].Animal Feed Science and Technology,2009,154(1):58-67.

[12] LIU Q,WANG C,HUANG Y X,et al.Effects of isovalerate on ruminal fermentation,urinary excretion of purine derivatives and digestibility in steers[J].Journal of Animal Physiology and Animal Nutrition,2009,93(6):716-725.

[13] WANG C,LIU Q,PEI C X,et al.Effects of 2-methylbutyrate on rumen fermentation,ruminal enzyme activities,urinary excretion of purine derivatives and feed digestibility in steers[J].Livestock Science,2012,145(1/2/3):160-166.

[14] 杨胜.饲料分析及饲料质量检测技术[M].北京:北京农业大学出版社,1993:16-35.

[15] 中华人民共和国农业部. NY/T34—2004奶牛饲养标准[S].北京:中国农业出版社,2004.

[16] 季香,马月辉,叶绍辉,等.反刍动物生长激素受体基因的研究进展[J].草食家畜,2006(2):27-31.

[17] HAMAMDZIC M.Recent knowledge on the role of isoacids in rumen metabolism and milk production[J].Veterinaria (Sarajevo),1989,38:3-17.

[18] 张斌,刘强,杨文柱,等.异丁酸钠对奶牛泌乳性能和血液指标的影响[J].饲料工业,2010,31(7):32-36.

[19] ROTHER K I,ACCILI D.Role of insulin receptors and IGF receptors in growth and development[J].Pediatric Nephrology,2000,14(7):558-561.

[20] 祝兴林,王哲,夏成,等.胰岛素受体mRNA在新生犊牛组织中的表达[J].中国兽医科技,2005,35(10):786-789.

[21] MATSUNAGA N,ARAKAWA N T,GOKA T,et al.Effects of ruminal infusion of volatile fatty acids on plasma concentration of growth hormone and insulin in sheep[J].Domestic Animal Endocrinology,1999,17(1):17-27.

[22] LANE M A,BALDWIN R L,JESSE B W.Developmental changes in ketogenic enzyme gene expression during sheep rumen development[J].Journal of Animal Science,2002,80(6):1538-1544.

[23] KELLETT G L.The facilitated component of intestinal glucose absorption[J].The Journal of Physiology,2001,531(3):585-595.

[24] RODRIGUEZ S M,GUIMARAES K C,MATTHEWS J C,et al.Influence of abomasal carbohydrates on small intestinal sodium-dependent glucose cotransporter activity and abundance in steers[J].Journal of Animal Science,2004,82(10):3015-3023.

[25] HUME I D.Synthesis of microbial protein in the rumen.Ⅱ.A response to higher volatile fatty acids[J].Australian Journal of Agricultural Research,1970,21(2):297-304.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References