Effects of Different Neutral Detergent Fiber to Starch Raito Diets on Rumen Fermentation in Vitro

ZHU Dan; ZHANG Peihua; ZHAO Meng; LIU Shijie; ZHANG Kaizhan; BU Dengpan; WILLIAM P. Weiss

doi:10.3969/j.issn.1006-267x.2015.08.032

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 8: 2580 - 2588

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.08.032

Molecular Nutrition

Effects of Different Neutral Detergent Fiber to Starch Raito Diets on Rumen Fermentation in Vitro

ZHU Dan ,
ZHANG Peihua ,
ZHAO Meng ,
LIU Shijie ,
ZHANG Kaizhan ,
BU Dengpan ,
WILLIAM P. Weiss

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
3. CAAS-ICRAF Joint Laboratory on Agroforestry and Sustainable Animal Husbandry, Beijing 100081, China;
4. Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agriculture University, Harbin 150030, China;
5. China Feed Industry Association, Beijing 100125, China;
6. Beijing Sino Farm Ltd., Co., Beijing 100028, China;
7. College of Animal Science and Technology, Ohio State University, Wooster 44691, USA

Received date: 2015-03-16

Online published: 2015-08-13

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Abstract

This experiment was conducted to examine the effects of different neutral detergent fiber to starch ratio (NDF/starch) diets on rumen fermentation in vitro. Corn silage, dried oat hay and corn were used to adjust the NDF/starch of total mixed rations, and NDF/starch was 0.86 (Ⅰ), 1.13 (Ⅱ), 1.56(Ⅲ) and 2.38 (Ⅳ), respectively. Three healthy Holstein cows fitted with permanent ruminal cannulas were used as the donor of rumen fluid. Accumulative gas production in 2,6,12,24,36 and 72 h, gas production kinetic parameters and rumen fermentation parameters of the diets were determined by combining the method of in vitro rumen fermentation and the technique of real-time recording of gas production. The results showed as follows: 1) with the increase of dietary NDF/starch, in vitro dry matter degradation rate after 48 h fermentation was decreased, and groups Ⅰ and Ⅱ were significantly higher than groups Ⅲ and Ⅳ (P<0.05); accumulative gas production in 48 h and theoretical maximum gas production ranked in descending order, and group Ⅳ was significantly higher than the other groups (P<0.05). No significant differences in the time of reaching 1/2 theoretical maximum gas production and the maximum gas production rate among all groups were observed (P>0.05). 2) The concentrations of ammonia nitrogen (NH₃-N) and microbial protein in fermentation fluid were not affected by dietary NDF/starch (P>0.05). 3) With the increase of dietary NDF/starch, pH, acetate/propionate and acetate/total volatile fatty acid (TVFA) were significantly increased (P<0.05 or P<0.01), but TVFA, propionate, butyrate and propionate/TVFA were significantly decreased (P<0.05 or P<0.01). 4) Correlation between nutrient levels of different NDF/starch diets and fermentation parameters in vitro showed that the accumulative gas production in 48 h and theoretical maximum gas production were significantly positive related to the contents of starch, neutral detergent soluble (NDS) and non-fiber carbohydrate(NFC), as well as NDS/CP (P<0.01), and also were significantly negative related to neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents (P<0.01). In conclusion, the results of in vitro gas production method indicate that dietary NDF/starch between 0.86 and 1.13 can promote rumen fermentation under the present experimental conditions.

Key words： neutral detergent fiber; starch; diets; rumen fermentation; in vitro

Cite this article

ZHU Dan , ZHANG Peihua , ZHAO Meng , LIU Shijie , ZHANG Kaizhan , BU Dengpan , WILLIAM P. Weiss . Effects of Different Neutral Detergent Fiber to Starch Raito Diets on Rumen Fermentation in Vitro[J]. Chinese Journal of Animal Nutrition, 2015 , 27(8) : 2580 -2588 . DOI: 10.3969/j.issn.1006-267x.2015.08.032

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