Cloning and Sequence Analysis of Full-Length cDNAs of Food Intake Regulation Factors in Japanese Seabass (Lateolabrax japonicus)

LIU Yan; XU Henglong; XUE Min; WANG Jia; WU Xiufeng; ZHENG Yinhua; HAN Fang; ZHANG Yanjiao

doi:10.3969/j.issn.1006-267x.2015.09.014

2015 , Vol. 27 >Issue 9: 2757 - 2773

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.09.014

Aquaculture Nutrition

Cloning and Sequence Analysis of Full-Length cDNAs of Food Intake Regulation Factors in Japanese Seabass (Lateolabrax japonicus)

LIU Yan ,
XU Henglong ,
XUE Min ,
WANG Jia ,
WU Xiufeng ,
ZHENG Yinhua ,
HAN Fang ,
ZHANG Yanjiao

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1. Microbial Ecology Laboratory, Ocean University of China, Qingdao 266003, China;
2. National Aquafeed Safety Assessment Station, Feed Reseach Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
3. Key Laboratory of Feed Biotechnology (Ministry of Agriculture), Beijing 100081, China;
4. Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) & The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, China

Received date: 2015-03-26

Online published: 2015-09-12

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Abstract

The full-length cDNAs of food intake regulation factors, including mechanistic target of rapamycin (mTOR), ribosomal protein S6 kinase beta-1 (S6K1), neuropeptide Y (NPY) precursor and preproghrelin were cloned from Japanese seabass (Lateolabrax japonicus) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE) methods. The results showed as follows: the full-length mTOR cDNA of Japanese seabass (GenBank accession No. KJ746670) was 8 296 bp, consisting of an open reading frame (ORF) of 7 551 bp encoding a polypeptide of 2 516 amino acids with a theoretical molecular weight of 286.14 ku, and shared 62.0% to 98.0% similarity with other species. The full-length S6K1 cDNA of Japanese seabass (GenBank accession No. KJ746671) was 2 232 bp, consisting of an ORF of 1 539 bp encoding a polypeptide of 512 amino acids with a theoretical molecular weight of 56.87 ku, and shared 80.4% to 84.9% similarity with other species. The full-length NPY precursor cDNA of Japanese seabass (GenBank accession No. KJ850326) was 676 bp, consisting of an ORF of 300 bp encoding a polypeptide of 99 amino acids with a theoretical molecular weight of 11.26 ku, and shared 53.6% to 99.0% similarity with other species. The full-length preproghrelin cDNA of Japanese seabass (GenBank accession No. KJ850327) was 1 201 bp, consisting of an ORF of 324 bp encoding a polypeptide of 107 amino acids with a theoretical molecular weight of 12.03 ku, and shared 19.6% to 85.0% similarity with other species. The results of this study will provide a scientific basis for further study to explain regulatory mechanism of the factors of food intake regulation in Japanese seabass.

Key words： gene cloning; food intake regulation; Japanese seabass (Lateolabrax japonicus); mTOR; S6K1; NPY precursor; preproghrelin

Cite this article

LIU Yan , XU Henglong , XUE Min , WANG Jia , WU Xiufeng , ZHENG Yinhua , HAN Fang , ZHANG Yanjiao . Cloning and Sequence Analysis of Full-Length cDNAs of Food Intake Regulation Factors in Japanese Seabass (Lateolabrax japonicus)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(9) : 2757 -2773 . DOI: 10.3969/j.issn.1006-267x.2015.09.014

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