Aquaculture Nutrition

Effects of Replacement of Fish Meal with Silkworm Pupae on Growth Performance, Body Composition and Serum Biochemical Indices of Genetically Improved Farmed Tilapia (Oreochromis niloticus)

  • WANG Shuwen ,
  • HUANG Xianzhi ,
  • LUO Li ,
  • CHEN Yongjun ,
  • BAI Fujin ,
  • LI Yun
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  • 1. College of Animal Science and Technology of Southwest University, Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratory of Aquatic Science of Chongqing, Chongqing 400715, China;
    2. State Key Laboratory of Silkworm Genome Biology, Chongqing 400716, China

Received date: 2015-03-27

  Online published: 2015-09-12

Abstract

This experiment was conducted to investigate the effects of different proportion silkworm pupae (SP) substitution for fish meal on growth performance, body composition and serum biochemical indices of genetically improved farmed tilapia (GIFT, Oreochromis niloticus). Five isonitrogenous (containing 32% crude protein) and isolipidic (containing 5.5%ether extract) diets were formulated by replacing 0 (control), 25%, 50%, 75% and 100% FM with SP on the basis of a basal diet which contained 8% fish meal, and they were named as SP0, SP25, SP50, SP75 and SP100, respectively. Each diet was fed to three replicates of 20 juvenile GIFT with an initial body weight of (44.4±0.41) g for 56 d. The results showed as follows: 1) compared with SP0 group, weight gain rate and specific growth rate in SP50 group were increased by 16.18% and 8.37% (P<0.05), respectively, and feed conversion ratio was decreased by 3.94% (P<0.05); no difference was found in viscerosomatic index among all groups (P>0.05). 2) Whole fish and hepatosomatic ether extract contents in SP50 groups were decreased by 6.39% and 7.76% compared with SP0 group (P<0.05), respectively, but whole body crude protein and hepatosomatic mositure contents were not influenced by silkworm pupae substitution proportion (P>0.05). 3) Protein efficiency ratio (PER) and protein retention rate (PRR) firstly increased and then decreased with increase of SP substitution proportion, and reached maximum in SP50 group which were increased by 6.48% and 13.83% compared with SP0 group (P<0.05), respectively; while lipid retention rate (LRR) followed contrary pattern as that of PER and PRR (P<0.05). 4) Replacement of FM with SP significantly decreased serum total cholesterol, triglyceride and glucose concents (P<0.05); serum lysozyme activity was the highest when the silkworm pupae substitution proportion was 50%, but it significantly declined when the silkworm pupae substitution proportion was over 75% (P<0.05), concomitantly, it significantly increased serum aspartate transaminase and alanine transaminase activities (P<0.05). In conclusion, replacement of 50% fish meal with silkworm pupae can improve growth performance, protein retention rate and serum lysozyme activity of GIFT, and has the functions of decreasing serum lipid,blood glucose and liver lipid.

Cite this article

WANG Shuwen , HUANG Xianzhi , LUO Li , CHEN Yongjun , BAI Fujin , LI Yun . Effects of Replacement of Fish Meal with Silkworm Pupae on Growth Performance, Body Composition and Serum Biochemical Indices of Genetically Improved Farmed Tilapia (Oreochromis niloticus)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(9) : 2774 -2783 . DOI: 10.3969/j.issn.1006-267x.2015.09.015

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