Effects of Fibrolytic Enzymes and Isobutyrate on Enzyme Activities and Cellulolytic Bacteria Flora in Rumen Fluid in Calves

XU Qianqian; HOU Ming; WANG Cong; LIU Qiang; ZHANG Yanli; PEI Caixia; WANG Yongxin; GUO Gang; HUO Wenjie

doi:10.3969/j.issn.1006-267x.2015.09.022

2015 , Vol. 27 >Issue 9: 2841 - 2848

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.09.022

Feed Science and Technology

Effects of Fibrolytic Enzymes and Isobutyrate on Enzyme Activities and Cellulolytic Bacteria Flora in Rumen Fluid in Calves

XU Qianqian ,
HOU Ming ,
WANG Cong ,
LIU Qiang ,
ZHANG Yanli ,
PEI Caixia ,
WANG Yongxin ,
GUO Gang ,
HUO Wenjie

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College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China

Received date: 2015-03-25

Online published: 2015-09-12

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Abstract

The objective of this study was to evaluate the effects of dietary fibrolytic enzymes and isobutyrate supplementation on enzyme activities and cellulolytic bacteria flora in pre- and post-weaning calves. Thirty-six healthy Holstein male calves with 30 days of age and similar body weight [(48.5±0.3) kg] were selected and allocated randomly to four groups. All calves were weaned at 60 days of age. Calves in control group were fed milk/diet for calves+dried alfalfa hay, and those in experimental groups were supplemented with fibrolytic enzymes (160 U filter paper enzyme and 4 000 U xylanase, 1.83 g/d, FE group), isobutyrate (99%, 6 g/d, IB group) and their mixture (IBFE group), respectively. Rumen fluid was collected in the morning before feeding at 45 and 90 days of age for determining rumen fermentation indices, enzyme activities and cellulolytic bacteria amount. The results showed that at 45 and 90 days of age, compared with control group, the concentrations of total volatile fatty acid, propionic acid and acetic acid in experimental groups were significantly increased (P<0.05); the concentration of ammonia nitrogen in IB and IBFE groups was significantly reduced (P<0.05); the activities of carboxymethyl cellulase, xylanase, filter paper enzyme and cellobiase in experimental groups were significantly increased (P<0.05), and the increasing extends were the highest in IBFE group; the amount of F. Succinogenes, R. albus, R. flavefaciens and B. fibrisolvens in experimental groups was significantly increased (P<0.05), and the increasing extend was the highest in IBFE group. Base on above results, 6 g/d isobutyric acid and 1.83 g/d fibrolytic enzymes have promoting effects on rumen fermentation, and the better is their combination.

Key words： fibrolytic enzyme; isobutyrate; rumen fermentation; enzyme activity; cellulolytic bacteria

Cite this article

XU Qianqian , HOU Ming , WANG Cong , LIU Qiang , ZHANG Yanli , PEI Caixia , WANG Yongxin , GUO Gang , HUO Wenjie . Effects of Fibrolytic Enzymes and Isobutyrate on Enzyme Activities and Cellulolytic Bacteria Flora in Rumen Fluid in Calves[J]. Chinese Journal of Animal Nutrition, 2015 , 27(9) : 2841 -2848 . DOI: 10.3969/j.issn.1006-267x.2015.09.022

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