Effect of Lactobacillus delbrueckii on Growth Performance, Nutrient Digestibility, Serum Biochemical Indexes and Intestinal Structure of Fattening Pigs

HOU Gaifeng; LI Rui; LIU Ming; WANG Houjun; PENG Wei; HUANG Xingguo

doi:10.3969/j.issn.1006-267x.2015.09.026

2015 , Vol. 27 >Issue 9: 2871 - 2877

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.09.026

Short Communications

Effect of Lactobacillus delbrueckii on Growth Performance, Nutrient Digestibility, Serum Biochemical Indexes and Intestinal Structure of Fattening Pigs

HOU Gaifeng ,
LI Rui ,
LIU Ming ,
WANG Houjun ,
PENG Wei ,
HUANG Xingguo

Expand

1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received date: 2015-03-20

Online published: 2015-09-12

Fold

Abstract

The study aimed at investigating the effects of Lactobacillus delbrueckii on growth performance, nutrient digestibility, serum biochemical indexes and intestinal structure of fattening pigs. A total of 120 healthy fattening pigs were randomly divided into 2 groups according to their body weight, and 6 replicates (pens) per group with 10 pigs (half male and half female) per replicate. The control group was fed basal diet (without antibiotic), while the experimental group was fed basal diet containing 0.1% Lactobacillus delbrueckii, and the diets were fed for 42 d. The fresh fecal samples of each pen were collected in the period from the day 12 to day 14 (earlier stage), day 26 to day 28 (middle stage) and day 40 to day 42 (later stage), respectively, and then collect anterior vena cava blood on the day 42, since the trial ended, one barrow with the similar body weight in each pen was slaughtered. The results were as follows: 1) the experimental group had a greater growth performance than the control group, but there was no significant difference (P>0.05). 2) Compared with the control group, during earlier and middle stages, the digestibility of crude protein (CP), extract ether (EE) and crude fiber (CF) were improved significantly (P0.05). In later stage, except for the digestibility of ash was significantly increased (P0.05). 3) The experimental group had higher content of serum urea and activities of alanine aminotransferase (ALT), alkaline phosphatase (ALP) and aspartate aminotransferase (AST), but there was no significant difference (P>0.05). 4) Compared with control group, the mucosal thickness of duodenum, jejunum and ileum was improved, but there was no significant difference (P>0.05), the crypt depth of duodenum was decreased significantly (P<0.05), the villous height of jejunum was increased extremely significantly (P<0.01), the villous height/crypt depth (V/C) of duodenum, jejunum and ileum was improved significantly or extremely significantly (P<0.05 or P<0.01). It is concluded that adding 0.1% Lactobacillus delbrueckii into antibiotic-free diet can ameliorate intestinal structure, promote the digestion, absorption and metabolism of nutrients, and improve growth performance of fattening pigs.

Key words： Lactobacillus delbrueckii; fattening pigs; growth performance; nutrient digestibility; serum biochemical indexes; intestinal structure

Cite this article

HOU Gaifeng , LI Rui , LIU Ming , WANG Houjun , PENG Wei , HUANG Xingguo . Effect of Lactobacillus delbrueckii on Growth Performance, Nutrient Digestibility, Serum Biochemical Indexes and Intestinal Structure of Fattening Pigs[J]. Chinese Journal of Animal Nutrition, 2015 , 27(9) : 2871 -2877 . DOI: 10.3969/j.issn.1006-267x.2015.09.026

References

[1] ALLEN H K,LEVINE U Y,LOOFT T,et al.Treatment,promotion,commotion:antibiotic alternatives in food-producing animals[J].Trends in Microbiology,2013,21(3):114-119.

[2] MENG Q W,YAN L,AO X,et al.Influence of probiotics in different energy and nutrient density diets on growth performance,nutrient digestibility,meat quality,and blood characteristics in growing-finishing pigs[J].Journal of Animal Science,2010,88(10):3320-3326.

[3] SUO C,YIN Y S,WANG X N,et al.Effects of Lactobacillus plantarum ZJ316 on pig growth and pork quality[J].BMC Veterinary Research,2012,8(1):89.
[4] HU Y L,DUN Y H,LI S N,et al.Dietary Enterococcus faecalis LAB31 improves growth performance,reduces diarrhea,and increases fecal Lactobacillus number of weaned piglets[J].PLoS One,2015,10(1):e0116635.
[5] 李瑞,侯改凤,黄其永,等.德氏乳杆菌对哺乳仔猪生长性能、血清生化指标、免疫和抗氧化功能的影响[J].动物营养学报,2013,25(12):2943-2950.
[6] XIE N,CUI Y,YIN Y N,et al.Effects of two Lactobacillus strains on lipid metabolism and intestinal microflora in rats fed a high-cholesterol diet[J].BMC Complementary and Alternative Medicine,2011,11(1):53.
[7] 邓军,李云锋,杨倩.枯草芽孢杆菌和猪源乳酸杆菌混合饲喂对仔猪肠绒毛发育的影响[J].畜牧兽医学报,2013,44(2):295-301.
[8] 曾东,李涛,倪学勤,等.乳酸杆菌和囊素三肽对肉鸡生长性能、屠宰性能、肉质和血清生化指标的影响[J].动物营养学报,2014,26(5):1320-1326.
[9] KIM G B,SEO Y M,KIM C H,et al.Effect of dietary prebiotic supplementation on the performance,intestinal microflora,and immune response of broilers[J].Poultry Science,2011,90(1):75-82.

[10] 刘辉,季海峰,张董燕,等.饲粮添加短乳杆菌对生长猪生长性能和血清生化指标的影响[J].动物营养学报,2013,25(1):182-189.
[11] 李涛,曾东,倪学勤,等.乳酸杆菌发酵饲料对猪生长性能和肉质及血清抗氧化性能的影响[J].湖南农业大学学报:自然科学版,2014,40(2):192-195.
[12] 胡新旭,周映华,卞巧,等.无抗发酵饲料对生长育肥猪生产性能、血液生化指标和肉品质的影响[J].华中农业大学学报,2015,34(1):72-77.
[13] 李瑞,侯改凤,黄兴国,等.乳酸菌的生物学功能及其在仔猪生产中的应用[J].中国饲料,2013(5):7-9,19.
[14] YANG F J,HOU C L,ZENG X F,et al.The use of lactic acid bacteria as a probiotic in swine diets[J].Pathogens,2015,4(1):34-45.

[15] 黄其永.德氏乳杆菌对哺乳仔猪消化器官及消化酶活性的影响研究[D].硕士学位论文.长沙:湖南农业大学,2013.
[16] 李瑞,侯改凤,邬理洋,等.微生态制剂对生长猪生产性能、氮磷排放量及血清免疫指标的影响[J].家畜生态学报,2013,34(6):66-71.
[17] 黄金华,梁珠民,宁国信,等.复合益生菌制剂对断奶仔猪的生长性能、养分消化率和血清生化指标的影响[J].饲料工业,2014,35(12):27-31.
[18] 金宇,胡宏章.AST/ALT比值与肝硬化的相关性探讨[J].热带医学杂志,2011,11(10):1144-1146.
[19] 张桐,徐奇友,许红,等.饲料中维生素D₃对松浦镜鲤幼鱼体成分和血清碱性磷酸酶的影响[J].华北农学报,2011,26(增刊):258-263.
[20] 张波.305例不同类型肾炎病人血清胱抑素C(CysC)与尿素(Urea)、肌酐(Cr)测定分析[J].内蒙古医科大学学报,2014,36(增刊):118-119.
[21] 伍淳操,王建华.乳酸菌和甘露寡糖对断奶仔猪生长及血清生化指标的影响[J].江苏农业学报,2011,27(1):94-99.
[22] EI AIDY S,VAN DEN BOGERT B,KLEEREBEZEM M.The small intestine microbiota,nutritional modulation and relevance for health[J].Current Opinion in Biotechnology,2015,32:14-20.
[23] 印遇龙.仔猪营养学[M].北京:中国农业出版社,2010.
[24] 冯跃进.热应激对猪和大鼠肠道结构和功能的影响及其修复机制[D].硕士学位论文.北京:中国农业科学院,2014.
[25] 章文明,汪海峰,刘建新.乳酸杆菌益生作用机制的研究进展[J].动物营养学报,2012,24(3):389-396.
[26] 王华,张淑梅,钟颖婷,等."乳菌宝"益生菌剂对断奶仔猪肠道生理生化指标的影响[J].东北农业大学学报,2013,44(12):27-31.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References