This experiment was conducted to investigate the effects of Bacillus magaterium 1259 on microbial flora in different gastrointestinal parts of laying hens. A total of 128 healthy New Yangzhou laying hens aged 45 weeks were divided randomly into 2 groups. The control group was fed basal diet and the experimental group was fed basal diet plus 2×106 CFU/g Bacillus magaterium 1259. The pre-experiment period was 1 week and the experimental period was 3 weeks. The fecal and intestinal samples were collected for microflora contents detections by quantitative real-time PCR (RT-PCR) at weekend of the first, second and third weeks during the experimental period. The results showed as follows: 1) the content of Salmonella bacteria in small intestinal contents at the second and third weeks of the experimental group significantly decreased (P<0.05), while the contents of Bacillus bifidus at the first and second weeks and Bacillus magaterium at the second and third weeks significantly increased (P<0.05). 2) The content of Bacillus magaterium in cecum contents of the experimental group significantly increased (P<0.05). 3) The contents of Escherichia coli, Salmonella bacteria in faeces of the experimental group significantly decreased (P<0.05), while the content of Bacillus magaterium at the second and third weeks significantly increased (P<0.05). 4) With the feeding time increased, the contents of Salmonella bacteria in small intestinal contents and faeces of the experimental group significantly decreased (P<0.05), the content of Bacillus magaterium in small intestinal, cecum contents and faeces significantly increased (P<0.05). 5) In addition, the beneficial bacteria increased and the harmful bacteria decreased with feeding time increased. In conclusion, Bacillus magaterium 1259 can effectively inhibit the growth of harmful bacteria such as Escherichia coli but promote the growth of lactic acid-producing bacteria such as Lactobacillus.
GU Tiantian
,
ZHU Pengfei
,
WANG Chunyuan
,
WANG Mengzhi
,
ZHANG Yanyun
. Effects of Bacillus Magaterium 1259 on Microbial Flora in Different Gastrointestinal Parts of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2015
, 27(9)
: 2894
-2902
.
DOI: 10.3969/j.issn.1006-267x.2015.09.029
[1] 袁杰利,文姝,康白,等.三种益生菌对种鸡肠内环境及生产性能的影响[J].中国微生态学杂志,1998,10(2):124-126.
[2] 郑学斌,杜江,余先祥,等.益生素对蛋鸡生产性能、生化指标及蛋品质的影响[J].兽药与饲料添加剂,2008,13(5):1-3.
[3] 王芳,张变英,武霞,等.复合益生素对蛋鸡生产性能及肠道菌群的影响[J].山西农业科学,2012,40(3):276-279.
[4] 李丽红,郭宏,马秋刚,等.芽孢杆菌复合微生态制剂对蛋鸡生产性能的影响[J].中国畜牧杂志,2005,41(9):48-49.
[5] 李晓刚.巨大芽孢杆菌降低蛋鸡排泄物中氨和硫化氢机理的研究[D].硕士学位论文.扬州:扬州大学,2012:34-37.
[6] 刘超男,郑世民,张春丽.微生态制剂及其在动物生产上的应用[J].动物医学进展,2003,24(4):52-54.
[7] 李研东,韩雪,王颖,等.动物微生态制剂的研究进展[J].饲料研究,2008(2):22-24.
[8] 李俊波,成廷水,吕武兴,等.枯草芽孢杆菌制剂对蛋鸡生产性能、蛋品质和养分消化率的影响[J].中国家禽,2009,31(4):15-17.
[9] 戴承镛.巨大芽孢杆菌及其制备方法、用途:中国,CN200510091277.0[P].2006-06-14.
[10] 李晓刚,顾欢,孙龙生,等.巨大芽孢杆菌1259对蛋鸡生产性能、养分消化率及血清指标的影响[J].江苏农业科学,2012,40(3):171-173.
[11] 奥斯伯 F M,布伦特R,金斯顿 R E,等.精编分子生物学实验指南[M].颜子颖,王海林 译.北京:科学出版社,2001:39.
[12] 景翠,李福彬,赵驻军,等.日粮添加地衣芽孢杆菌对蛋鸡肠道菌群与形态结构的影响[J].中国家禽,2012,34(4):18-20.
[13] 陈国营,詹凯,朱由彩,等.枯草芽孢杆菌及其发酵豆粕对蛋鸡肠道菌群和粪便中N、S含量的影响[J].中国家禽,2012,34(6):10-15.
[14] 吕尊周,袁肖笑,郝柱,等.芽孢杆菌制剂对蛋鸡产蛋性能、血清相关指标及肠道菌群的影响[J].中国兽医学报,2012,32(7):1068-1072.
[15] 张华,杨雪,曹喜春,等.芽孢杆菌的生理功能及其在畜禽生产中的应用[J].饲料博览,2013(6):29-31.
[16] 杨承剑,黄兴国.微生态制剂及其在畜牧生产中的应用[J].饲料博览,2006(2):9-13.
[17] 丁轲,余祖华,倪学勤.乳酸杆菌和中草药合生元添加剂对肉鸡生长性能的影响[J].兽药与饲料添加剂,2007,12(6):1-4.
[18] 顾金,周维仁,闫俊书,等.微生态制剂对鸡肠道菌群调控的研究[J].饲料研究,2010(1):16-18.
[19] 周小辉,李彪.枯草芽孢杆菌的机制及应用研究[J].饲料研究,2009(10):26-28.
[20] 程安春,何明清,陈孝跃.SA38蜡样芽孢杆菌在体外对几种致病菌的生物拮抗试验[J].中国兽医杂志,1994,20(2):12-13.
[21] 全艳玲.地衣芽孢杆菌对有害微生物的拮抗作用[J].食品科学,2002,23(8):67-68.
[22] 冯亚强,薛恒平.芽孢杆菌微生态制剂的应用进展[J].中国饲料,1996(10):8-10.
[23] 吕道俊,何明清.饲用芽孢杆菌研究进展(综述)[J].四川农业大学学报,1994,12(增刊):635-638.
[24] 安永义,王新谋,刘春燕,等.活菌添加剂对肉仔鸡粪臭和肉仔鸡生产性能影响的研究[J].饲料研究,1996(12):1-17.
[25] 饶正华.饲料中沙门氏菌的检验方法[J].饲料博览,2002(3):25-28.
