Aquaculture Nutrition

Lysine Requirement of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)

Expand
  • Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2014-11-30

  Online published: 2015-10-15

Abstract

In this experiment, six isonitrogenous and isolipidic practical diets (contained 45.00% crude protein and 7.00% crude lipid) were formulated with dietary lysine supplement levels of 0, 0.30%, 0.60%, 0.90%, 1.20% and 1.50%, and the measured values of dietary lysine levels were 1.58%, 1.89%, 2.15%, 2.41%, 2.67% and 2.90%, respectively, in order to evaluate the effects of dietary lysine level on growth performance, nutritional components and blood indexes of juvenile yellow catfish (Pelteobagrus fulvidraco), and to determine the lysine requirement of juvenile yellow catfish.A total of 360 yellow catfish with an average initial body weight about 2.00 g were randomly allocated to 6 groups with 3 replicates per group and 20 juvenile yellow catfish per replicate.The experiment lasted for 12 weeks.The results showed as follows: the survival ratio (SR) in 1.58% group was significantly lower than that in other groups (P<0.05).With the dietary lysine level increasing, the weight gain rate (WGR) and specific growth rate (SGR) were firstly increased and then decreased, and the highest values of WGR and SGR were found in 2.41% group.The lowest values of feed efficiency (FE) and protein efficiency rate (PER) were found in 1.58% group, and significantly lower than those in other groups except 2.15% group (P<0.05).The condition factor (CF), hepatosomatic index (HSI) and viscerosomatic index (VSI) were not significantly affected by dietary lysine level (P>0.05).There were no significant differences in the contents of whole body dry matter and crude lipid and muscle dry matter, crude lipid and crude protein among all groups (P>0.05), but the contents of whole body ash and crude protein and muscle ash were significantly affected by dietary lysine level (P<0.05), and the lowest contents of whole body and muscle ash and the highest content of whole body crude protein were all found in 2.41% group.The whole blood red blood cell count, white blood cell count, hemoglobin content and hematocrit were not significantly affected by dietary lysine level (P>0.05), and the serum total cholesterol and glucose contents and aspertate amino transferase (AST) activity were also not significantly affected by dietary lysine level (P>0.05), but the serum triglyceride content and alanine amino transferase (ALT) activity were significantly affected by dietary lysine level (P<0.05).Base on the linear model with the WGR as an evaluation index, the lysine requirement of juvenile yellow catfish is 2.61% of diet (5.80% of dietary protein).

Cite this article

QIU Hong, HUANG Wenwen, HOU Yingmei, LI Yi, ZHOU Qicun . Lysine Requirement of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(10) : 3057 -3066 . DOI: 10.3969/j.issn.1006-267x.2015.10.010

References

[1] 陈代文.饲料添加剂学[M].北京:中国农业出版社,2003:35-36.

[2] WALTON M J,COWEY C B,ADRON J W.The effect of dietary lysine levels on growth and metabolism of rainbow trout (Salmo gairdneri)[J].British Journal of Nutrition,1984,52(1):115-122.  

[3] SANDELL L J,DANIEL J C.Effects of ascorbic acid on collagen mRNA levels in short term chondrocyte cultures[J].Connect of Tissue Research,1988,17(1):11-22.  

[4] 李爱杰.水产动物营养与饲料[M].北京:中国农业出版社,1998:18-26.

[5] SMALL B C,SOARES J H.Quantitative dietary lysine requirement of juvenile striped bass Morone saxatilis[J].Aquaculture Nutrition,2000,6(4):207-212.  

[6] WILSON R P,POE W E.Relationship of whole body and egg essential amino acid patterns to amino acid requirement patterns in channel catfish,Ictalurus punctatus[J].Comparative Biochemistry and Physiology Part B:Comparative Biochemistry,1985,80(2):385-388.

[7] 黄更生,李贵生,周黎华.草鱼生长阶段对赖氨酸需要量的研究[J].生态科学,2003,22(2):147-149.

[8] 赵春荣.赖氨酸对幼建鲤消化能力和免疫功能的影响[D].硕士学位论文.雅安:四川农业大学,2005:44-51.

[9] 杨青松.尼罗罗非鱼饲料赖氨酸和蛋氨酸需求量的探讨[J].福建水产,1992(1):67-70.

[10] 林郁葱.胭脂鱼幼鱼对赖氨酸和蛋氨酸的最适需要量[D].硕士学位论文.武汉:华中农业大学,2012:19-27.

[11] 周贤君,解绶启,谢从新.异育银鲫幼鱼对饲料中赖氨酸的利用及需要量研究[J].水生生物学报,2006,30(3):247-255.

[12] 王渊源.鱼虾的蛋白质需要量和其研究方法[J].动物学杂志,1991,26(5):26-42.

[13] CHENG Z J,HARDY R W,USRY J L.Effects of lysine supplementation in plant protein-based diets on the performance of rainbow trout (Oncorhynchus mykiss) and apparent digestibility coefficients of nutrients[J].Aquaculture,2003,215(1/2/3/4):255-265.

[14] 谭芳芳.在草鱼(Ctenopharyngodon idellus)饲料中补充微囊和晶体氨基酸的研究[D].硕士学位论文.苏州:苏州大学,2010.

[15] 王洪涛.异育银鲫对饲料中单体赖氨酸和蛋氨酸的利用研究[D].硕士学位论文.青岛:中国海洋大学,2009:15-19.

[16] ZHU W,MAI K S,ZHANG B G,et al.A study on the meat and bone meal and poultry by-product meal as protein substitutes of fish in practical diets for Patalichthys olivaceus[J].Journal of Ocean University of China,2006,5(1):63-66.  

[17] 张满隆,何小慧.鲤鱼饲料中添加赖氨酸的实验[J].饲料研究,2001(6):31-32.

[18] 尹海富,孙辉,韩英,等.单体氨基酸在水产饲料中的应用[J].中国水产,2002(2):68-69.

[19] 文远红,曹俊明,黄燕华,等.蝇蛆粉替代鱼粉对黄颡鱼幼鱼生长性能、体组成和血浆生化指标的影响[J].动物营养学报,2013,25(1):171-181.

[20] 李明锋.黄颡鱼人工养殖的可行性及实用技术[J].饲料研究,2000(11):40-41.

[21] 沈志刚.黄颡鱼蛋白质需求及饲料配方[J].齐鲁渔业,2010,27(4):38-40.

[22] 韩庆,田宗城,夏维福,等.黄颡鱼饲料脂肪的最适含量[J].水产科学,2005,24(7):8-11.

[23] 韩庆,夏维福,罗玉双,等.不同营养水平对黄颡鱼春片鱼种生长的影响[J].饲料工业,2002,23(7):43-44.

[24] AOAC.Official methods of analysis of AOAC[S].16th ed.Arlington,VA:Association of Analytical Chemist,Inc.,1995.

[25] 谢帝芝,许宝红,刘巧林,等.水生动物赖氨酸营养研究[J].湖南饲料,2010(2):17-19.

[26] ZHOU Q C,WU Z H,CHI S Y,et al.Dietary lysine requirement for juvenile cobia (Rachycentron canadum)[J].Aquaculture,2007,273(4):634-640.  

[27] ZHANG C X,AI Q H,MAI K S.Dietary lysine requirement of large yellow croaker,Pseudosciaena crocea R[J].Aquaculture,2008,283(1/2/3/4):123-127.

[28] PERES H,OLIVA-TELES A.Lysine requirement and efficiency of lysine utilization in turbot (Scophthalmus maximus) juvenile[J].Aquaculture,2008,275(1/2/3/4):283-290.

[29] LIN Y C,GONG Y,YUAN Y C,et al.Dietary L-lysine requirement of juvenile Chinese sucker,Myxocyprinus asiaticus[J].Aquaculture Research,2013,44(10):1539-1549.  

[30] 杨威.鳡幼鱼赖氨酸需求量的研究[D].硕士学位论文.武汉:华中农业大学,2012.

[31] BICUDO Á J A,SADO R Y,CYRINO J E P.Dietary lysine requirement of juvenile pacu Piaractus mesopotamicus (Holmberg,1887)[J].Aquaculture,2007,297(1/2/3/4):151-156.

[32] ROBINSON E H,WILSON R P,POE W E.Re-ievaluation of the lysine requirement and lysine utilization by fingerling channel catfish[J].The Journal of Nutrition,1980,110(11):2313-2316.

[33] FORSTER I,OGATA H Y.Lysine requirement of juvenile Japanese flounder Paralichthys olivaceus and juvenile red sea bream Pagrus major[J].Aquaculture,1998,161(1/2/3/4):131-142.

[34] SIMMONS L,MOCCIA R D,BUREAU D P,et al.Dietary methionine requirement of juvenile Arctic charr Salvelinus alpinus[J].Aquaculture Nutrition,1999,5(2):93-100.  

[35] DE SILVA S S,GUNASEKERA R M,GOOLEY G.Digestibility and amino acid availability of three protein-rich ingredient-incorporated diets by murray cod Maccullochella peelii (Mitchell) and the Australian shortfin eel Anguilla australis Richardson[J].Aquaculture,2009,31(2):195-205.

[36] RODEHUTSCORD M,BECKER A,PACK M,et al.Response of rainbow trout (Oncorhynchus mykiss) to supplements of individual essential amino acids in a semipurified diet,including an estimate of the maintenance requirement for essential amino acids[J].The Journal of Nutrition,1997,127(6):1166-1175.

[37] KIM K I,KAYES T B,AMUNDSON C H.Requirements for lysine and argnine by rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,1992,106(3/4):333-344.

[38] 朱选,曹俊明,许丰孟,等.饲料中添加赖氨酸及蛋氨酸对罗非鱼生长的影响[J].食品与生物技术学报,2009,28(4):465-473.

[39] 周萌,吴建开,曹俊明,等.军曹鱼幼鱼对赖氨酸需要量的初步研究[J].长江大学学报:自然科学版,2005,2(2):50-52.

[40] 杜强.卵形鲳鲹赖氨酸和蛋氨酸需求量及饲料中鱼粉替代的研究[D].硕士学位论文.上海:上海海洋大学,2012.

[41] 程成荣,温小波,付志雄,等.异育银鲫饲料中补充晶体氨基酸对生长及体组成的影响[J].饲料工业,2010,31(16):19-22.

[42] CHENG Z J,HARDY R W,USRY J L.Plant protein ingredients with lysine supplementation reduce dietary protein level in rainbow trout (Oncorhynchus mykiss) diets,and reduce ammonia nitrogen and soluble phosphorus excretion[J].Aquaculture,2003,218(1/2/3/4):553-565.

[43] DE ALMEIDA BICUDO Á J,CYRINO J E P.Estimating amino acid requirement of Brazilian freshwater fish from muscle amino acid profile[J].Journal of the World Aquaculture Society,2009,40(6):818-823.  

[44] ZHANG Y Z,ZHOU F,SHAO Q J,et al.Study on lysine requirement of juvenile black sea bream (Sparus macrocephalus)[J].Chinese Journal of Animal Nutrition,2009,21(1):78-87.

[45] YAN Q,XIE S,ZHU X,et al.Dietary methionine requirement for juvenile rockfish,Sebastes schlegeli[J].Aquaculture Nutrition,2007,13(3):163-169.  

[46] 宋理平,韩勃,王爱英,等.碳水化合物水平对厚唇弱棘鯻生长和血液指标的影响[J].大连海洋大学学报,2010,25(4):293-297.
Outlines

/