Aquaculture Nutrition

Dietary Vitamin C Requirement of Juvenile Yellow Catfish (Pelteobagrus fulvidraco Rich)

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  • Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2015-04-24

  Online published: 2015-10-15

Abstract

A 12-week feeding trial was conducted to evaluate the effects of dietary vitamin C level on growth performance, antioxidant capacity and immune function of juvenile yellow catfish (Pelteobagrus fulvidraco Rich).Six isonitrogenous and isolipidic diets (44% crude protein and 7% crude lipid) were formulated to contain six graded dietary vitamin C levels of 1.9, 18.9, 36.8, 78.4, 156.5 and 316.0 mg/kg diet.Each diet was randomly assigned to three replicates, and ecah replicate had 20 juvenile fish [average body weight of (2.00±0.01) g].The results showed as follows: 1) the weight gain rate (WGR) and specific growth rate (SGR) were significantly increased when dietary vitamin C level increased from 1.9 mg/kg to 156.5 mg/kg (P<0.05), but then were decreased when dietary vitamin C level increased from 156.5 mg/kg to 316.0 mg/kg.However, survival rate (SR), protein efficiency ratio (PER), feed efficiency (FE), hepatosomatic index (HSI) and viscerosomatic index (VSI) were not significantly affected by the dietary vitamin C level (P>0.05).2) The dietary vitamin C level had significant effects on blood hemoglobin content and serum glucose and triglyceride contents (P<0.05), but had no significant effects on red blood cell count (RBC), white blood cell count (WBC) and hematocrit in blood and total protein (TP) and cholesterol (CHOL) contents in serum (P>0.05).3) The activities of serum superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px)were significantly increased and the content of serum malondialdehyde (MDA) was significantly decreased when dietary vitamin C level increased from 1.9 mg/kg to 156.5 mg/kg (P<0.05); however, when dietary vitamin C level increased from 156.5 mg/kg to 316.0 mg/kg, the serum SOD and CAT activities and MDA content were not significant different (P>0.05), but the serum GSH-Px activity was significantly decreased (P<0.05).4) The activity of serum lysozyme (LZM) was significantly increased when dietary vitamin C level increased from 1.9 mg/kg to 156.5 mg/kg (P<0.05), but it had no significant change when dietary vitamin C level continued to increase (P>0.05).The phagocytic percentage and respiratory burst of macrophages from head kidney were firstly increased and then decreased with dietary vitamin C level increasing, and the highest values of them were appeared when dietary vitamin C level was 156.5 mg/kg.5) After injecting A.hydrophila for 14 days, the cumulative survival rate of 18.89 and 36.76 mg/kg groups was significantly higher than that of 1.9 mg/kg group (P<0.05).Using SGR and serum LZM activity as evaluation indexes, broken-line regression analysis results indicate that the dietary vitamin C requirement of juvenile yellow catfish is estimated to be 114.5 and 142.3 mg/kg, respectively.

Cite this article

GUO Chunyang, QIU Hong, LIANG Xiongpei, ZHOU Qicun . Dietary Vitamin C Requirement of Juvenile Yellow Catfish (Pelteobagrus fulvidraco Rich)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(10) : 3067 -3076 . DOI: 10.3969/j.issn.1006-267x.2015.10.011

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