Feed Science and Technology

Effects of Different Kinds of Probiotics on Superoxide Dismutase Regulatory Mechanism of Laying Hens

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  • 1. Department of Animal Science, Yanbian University, Yanji 133002, China;
    2. Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China

Received date: 2015-04-14

  Online published: 2015-10-15

Abstract

This experiment was conducted to investigate the effects of different kinds of probiotics on superoxide dismutase (SOD) regulatory mechanism of laying hens.A total of 360 healthy Hyline laying hens were randomly divided into 5 groups with 3 replicates per group and 24 hens per replicate.The hens in control group (group Ⅰ) were fed a basal diet, and the others in experimental groups (groupsⅡ, Ⅲ, Ⅳ and Ⅴ) were fed the basal diets supplemented with 0.5% preparation of lactic acid bacteria with traditional Chinese medicine, 0.25% lactic acid bacteria preparation mixed with 0.25% Monascus preparations with traditional Chinese medicine, 0.5% lactic acid bacteria preparation and 0.5% Monascus preparations with traditional Chinese medicine, respectively.The experiment lasted for 79 days.The results showed as follows: 1)compared with the group Ⅰ, the serum total superoxide dismutase (T-SOD) activity of experimental groups was significantly increased (P<0.05), the yolk T-SOD activity of groups Ⅱ, Ⅲand Ⅳ was significantly increased (P<0.05).2) Compared with the group Ⅰ, the liver SOD1 mRNA expression of group Ⅱ was significantly increased (P<0.05), the liver SOD2 mRNA expression of group Ⅱ, Ⅳ and Ⅴ was significantly increased (P<0.05).In conclusion, different kinds of probiotics can regulate the mechanism on SOD of laying hens, and diets supplemented with 0.5% preparation of lactic acid bacteria with traditional Chinese medicine has the best result in this experiment.

Key words: probiotics; SOD; mRNA; laying hens

Cite this article

ZHANG Xin, JIN Xijiu, JIN Haiguo, ZHANG Min . Effects of Different Kinds of Probiotics on Superoxide Dismutase Regulatory Mechanism of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2015 , 27(10) : 3134 -3139 . DOI: 10.3969/j.issn.1006-267x.2015.10.019

References

[1] 武书庚,王晶,张海军,等.蛋鸡机体内氧化与抗氧化平衡研究进展及调控[C]//动物营养研究进展.北京:中国农业科学技术出版社,2012:128-139.

[2] 林海.家禽热应激状态下的营养与生理反应[C]//动物营养研究进展论文集.北京:中国农业科学技术出版社,2004:237-349.

[3] 刘栋,朱剑英,牛钟相.动物微生态制剂在家禽生产中的应用及其发展前景[J].家禽科学,2008(1):42-46.

[4] 尤淑广,柳军.中草药添加剂在蛋鸡生产上的应用[J].畜禽业,2011(6):26-28.

[5] 祝辉,倪学勤.有益菌中药合生元研究进展[C]//生态环境与畜牧业可持续发展学术研讨会暨中国畜牧兽医学会2012年学术年会和第七届全国畜牧兽医青年科技工作者学术研讨会会议论文集.北京:中国畜牧兽医学会,2012.

[6] D'SOUZA A,FORDJOUR L,AHMAD A,et al.Effects of probiotics,prebiotics,and synbiotics on messenger RNA expression of caveolin-1,NOS,and genes regulating oxidative stress in the terminal ileum of formula-fed neonatal rats[J].Pediatric Research,2010,67(5):526-531.  

[7] 刘晶,黄珊珊,赵征.乳酸菌抗氧化能力研究进展[J].中国乳品工业,2010,38(5):38-41.

[8] 陈卫,田丰伟,赵鑫,等.乳酸菌干预氧化应激的研究进展[J].中国食品学报,2012,12(11):1-7.

[9] 王全溪,林滉,贾洪强,等.乳酸菌素对肉鸡血清生化指标和抗氧化功能的影响[J].动物营养学报,2014,24(1):131-136.

[10] 董秀梅,张超范,魏萍.复合微生态制剂对肉仔鸡肠道菌群及抗氧化机能的影响[J].中国家禽,2004,26(l4):11-13.

[11] 崔明勋,宋光宇,姜成哲,等.红曲霉复合制剂对小鼠肝和肾抗氧化能力的影响[J].畜牧与饲料科学,2009,30(9):12-14.

[12] 丛明柱,张敏.红曲霉与中药合生素对延边黄牛血液指标的影响[J].黑龙江畜牧兽医,2011(5):80-81.

[13] 范明哲,张鑫,陈天龙,等.不同组成合生元对蛋鸡生产性能及蛋品质的影响[J].饲料广角,2014(21):24-27.

[14] 孙建晨.红曲霉合生元对蛋鸡生产性能、蛋品质及血液生化指标的影响[D].硕士学位论文.延吉:延边大学,2013.

[15] 平雨鑫.红曲霉中药复合制剂对鸡蛋品质的影响[D].硕士学位论文.延吉:延边大学,2012.

[16] 翁洋.倪学勤,曾东.有益菌和中药的协同作用及其应用[J].中国兽医杂志,2009,45(2):55-57.

[17] ELCHURI S,OBERLEY T D,QI W B,et al.CuZnSOD deficiency leads to persistent and widespread oxidative damage and hepatocarcinogenesis later in life[J].Oncogene,2005,24(3):367-380.  

[18] YI J F,LI Y M,LIU T,et al.Mn-SOD and CuZn-SOD polymorphisms and interactions with risk factors in gastric cancer[J].World Journal of Gastroenterology,2010,16(37):4738-4746.  

[19] ARCAROLI J J,HOKANSON J E,ABRAHAM E,et al.Extracellular superoxide dismutase haplotypes are associated with acute lung injury and mortality[J].American Journal of Respiratory and Critical Care Medicine,2009,179(2):105-112.  

[20] 杨茂平,叶林秀,邱红,等.锰超氧化物歧化酶9 Ala/Val基因多态性与冠心病的关系[J].武汉大学学报:医学版,2008,29(6):775-779.

[21] LI Y B,HUANG T T,CARLSON E J,et al.Dilated cardiomyopathy and neonatal lethality in mutant mice lacking manganese superoxide dismutase[J].Nature Genetics,1995,11(4):376-381.  
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