Effects of Different Type of Sodium Butyrate on Serum Biochemical Indices, Antioxidant and Anti-Inflammatory Function of Broilers Challenged with Lipopolysaccharide

JU Tingting, GUO Xiaoye, SUI Jiajia, XIAO Xue, ZHAN Xiuan

doi:10.3969/j.issn.1006-267x.2015.10.021

2015 , Vol. 27 >Issue 10: 3146 - 3154

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.10.021

Feed Science and Technology

Effects of Different Type of Sodium Butyrate on Serum Biochemical Indices, Antioxidant and Anti-Inflammatory Function of Broilers Challenged with Lipopolysaccharide

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College of Animal Science, Zhejiang University, Hangzhou 310058, China

Received date: 2015-04-14

Online published: 2015-10-15

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Abstract

The trial was conducted to study on the effects of different type of sodium butyrate on serum biochemical indices, antioxidant and anti-inflammatory function of broilers challenged with lipopolysaccharide (LPS).Feeding trial: a total of 1 000 one-day-old Lingnan yellow broilers were randomly allocated into 5 groups with 4 replicates in each group and 50 broilers per replicate.Broilers in control group were fed a basal diet, the others in experimental groups were fed the basal diets supplemented with 500 mg/kg powder sodium butyrate (NaB), and 250, 500 and 750 mg/kg coated sodium butyrate (CNaB), respectively.The LPS injection experiment was conducted followed a 50-day feeding trial, 72 fifty-day-old female broilers were selected randomly from control, NaB and 250 mg/kg CNaB group with the best growth performance, with 6 broilers per replicate.And they were allotted into a 2×3 factorial arrangement with challenge status (saline or LPS exposure) and feed diet (0, 500 mg/kg NaB or 250 mg/kg CNaB) as main factor for endotoxin exposure test.Half of broilers in each group were injected intraperitoneally with E.coli LPS (0.5 mg/kg body weight)at 53, 56 and 59 d of age, the remaining broilers were injected intraperitoneally with the same amount of saline as the LPS injected group.The trial lasted for 10 days.The results showed as follows: 1) compared with the saline injected group, the content of glucose and riglyceride in serum was significantly decreased in LPS injected group (P<0.05), but the serum uric acid content as significantly increased (P<0.05).The serum glucose content of NaB and CNaB groups injected with LPS was significantly higher than that of control group (P<0.05).2) Compared with the saline injected group, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum and liver was significantly decreased in LPS injected group (P<0.05 or P<0.01), the serum catalase (CAT) activity was significantly decreased (P<0.05).The activity of SOD and CAT in serum and liver of NaB and CNaB groups injected with LPS was higher than that of control group (P>0.05), but the malonaldehyde content was lower than that of control group (P>0.05).There were a significant interaction between dietary sodium butyrate and LPS injected on liver GSH-Px activity (P<0.05), and the two forms of sodium butyrate exerted an equal effect on antioxidant enzyme activity.3) Compared with the saline injected group, the content of ceruloplasmin and interleukin-6 in serum at the age of 53 and 59 days was significantly increased in LPS injected group (P<0.05).The serum ceruloplasmin content at the age of 53 days of NaB and CNaB groups injected with LPS was significantly lower than that of control group (P<0.05).In conclusion, sodium butyrate can improve the nutrient metabolism, maintain antioxidant function and promote anti-inflammation function, the effects on anti-stress of 500 mg/kg NaB are equal to those of 250 mg/kg CNaB which contains 75 mg/kg effective substance.

Key words： sodium butyrate; broilers; biochemical indices; antioxidant; anti-inflammation

Cite this article

JU Tingting, GUO Xiaoye, SUI Jiajia, XIAO Xue, ZHAN Xiuan . Effects of Different Type of Sodium Butyrate on Serum Biochemical Indices, Antioxidant and Anti-Inflammatory Function of Broilers Challenged with Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2015 , 27(10) : 3146 -3154 . DOI: 10.3969/j.issn.1006-267x.2015.10.021

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