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Bacteria Community Diversity of Liquid and Solid Phases of Ruminal Contents of Mongolian Sheep Analyzed by Illumina MiSeq Platform

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  • 1. Institute of Animal Microecology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China;
    2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya'an 625014, China

Received date: 2015-04-01

  Online published: 2015-10-15

Abstract

This experiment was conducted to compare bacteria community diversity of liquid and solid phases of ruminal contents of Mongolian sheep.The samples of liquid and solid phases of ruminal contents from 5 Mongolian sheep were investigated using 16S rRNA amplicon high-throughput sequencing technique by Illumina MiSeq platform, and the results were analyzed by UPARSE-operational taxonomic units (OTU) and unweighted pair group method with arithmetic mean (UPGMA).The results showed that a total of 91 971 sequences and 1 871 OTUs across all samples were observed, and the average number of sequence and OTU was 13 138 and 267 per sample, respectively.Compared between the samples of liquid and solid phases: at phyla level, dominant bacteria with significant differences were Bacteroidetes and Firmicutes, and the percentage of Bacteroidetes of the samples of liquid and solid phases was 67% and 45%, respectively; at genus level, dominant bacteria with significant differences were Prevotella and an unknown genus, dominant bacterium of liquid phase was Prevotella whose percentage reached 53%, and that of solid phase was the unknown genus whose percentage reached 32%; at species level, dominant bacteria with significant differences were Prevotella ruminicola, and the percentage of liquid and solid phases reached 41% and 11%, respectively.The alpha diversity of solid phase was higher compared with that of liquid phase, and the samples from the same animal clustered together by principal component analysis (PCA) and principal coordinate analysis (PCoA).In conclusion, both the contents of liquid and solid phases have high diversity of bacteria, and the diversity of solid phase is higher compared with that of liquid phase.

Cite this article

ZENG Yan, JIAN Ping, NI Xueqin, ZHOU Yi, ZHU Hui, ZENG Dong . Bacteria Community Diversity of Liquid and Solid Phases of Ruminal Contents of Mongolian Sheep Analyzed by Illumina MiSeq Platform[J]. Chinese Journal of Animal Nutrition, 2015 , 27(10) : 3256 -3262 . DOI: 10.3969/j.issn.1006-267x.2015.10.034

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