Effects of Dietary Vitamin C on Resistance in Edwardsiella tarda Infection of Tongue Sole (Cynoglossus semilaevis)

HUANG Yadong, SUN Mengmeng, DOU Yanjun, WANG Qingkui, CHEN Chengxun, XING Kezhi

doi:10.3969/j.issn.1006-267x.2015.10.035

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 10: 3263 - 3269

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.10.035

Short Communications

Effects of Dietary Vitamin C on Resistance in Edwardsiella tarda Infection of Tongue Sole (Cynoglossus semilaevis)

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1. Tianjin Haisheng Aquaculture Co., Ltd., Tianjin 300272, China;
2. Tianjin Ocean Pal Carol Biotech Co., Ltd., Tianjin 300356, China;
3. Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Department of Fishery Science, Tianjin 300384, China

Received date: 2015-04-28

Online published: 2015-10-15

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Abstract

This study was conducted to investigate the effects of vitamin C on red blood cell (RBC) antioxidation capacity and glucose-6-phosphate (G-6-PD) activity of tongue sole (Cynoglossus semilaevis).Six experimental diets were prepared with vitamin C content of 0.2 (control), 104.8, 209.6, 313.8, 521.6 and 837.3 mg/kg in form of L-ascorbyl-2-polyphosphate, respectively, and those diets were fed healthy tongue sole [average body weight of (99.12±1.58) g] for 84 days.After feeding trial, The test fish were challenged with Edwardsiella tarda for 14 days.The results showed as follows: the highest values of final body weight, special growth rate and weight gain rate were found in the 209.6 mg/kg group.With the dietary vitamin C content increasing, the vitamin C content in plasma, and hemoglobin (Hb) content and G-6-PD activity in RBC all showed an increasing tendency, and all additional groups were all significantly higher than the control group (P<0.05) except RBC G-6-PD activity in 104.8 mg/kg group.The SOD and CAT activities in RBC in 209.6 mg/kg group were significantly higher than those in the control group (P<0.05), and then the activities decreased with continued to increase the dietary vitamin C content.The malondialdehvde (MDA) content in RBC and nitric oxide (NO) in RBC membrane in the control group were significantly higher than those in all additional groups (P<0.05).The results indicate that the vitamin C can effectively reduce the oxidant damage of RBC of tongue sole with Edwardsiella tarda infection.The suitable dietary vitamin C content is 257.04 to 341.36 mg/kg which estimated by broken-line models.

Key words： tongue sole (Cynoglossus semilaevis); vitamin C; Edwardsiella tarda; RBC; RBC membrane; antioxidation capacity

Cite this article

HUANG Yadong, SUN Mengmeng, DOU Yanjun, WANG Qingkui, CHEN Chengxun, XING Kezhi . Effects of Dietary Vitamin C on Resistance in Edwardsiella tarda Infection of Tongue Sole (Cynoglossus semilaevis)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(10) : 3263 -3269 . DOI: 10.3969/j.issn.1006-267x.2015.10.035

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