Effects of Enteromorpha prolifera Adding Ratio and Microbial Fermentation on Growth, Digestion and Non-Specific Immune of Juvenile Sea Cucumbers (Apostichopus japonicus Selenka)

LI Meng, LIAO Meijie, WANG Yingeng, CHANG Qing, LI Bin, ZHANG Zhen, FAN Ruiyong

doi:10.3969/j.issn.1006-267x.2015.10.036

2015 , Vol. 27 >Issue 10: 3270 - 3278

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.10.036

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Effects of Enteromorpha prolifera Adding Ratio and Microbial Fermentation on Growth, Digestion and Non-Specific Immune of Juvenile Sea Cucumbers (Apostichopus japonicus Selenka)

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1. College of Fishers and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;
2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
3. Qingdao Haichang Aquatic Science & Technology Co., Ltd., Qingdao 266400, China;
4. Qingdao Ruizi Seafood Development Co., Ltd., Qingdao 266400, China

Received date: 2015-04-15

Online published: 2015-10-15

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Abstract

A 46-day feeding trial was conducted to evaluate the effects of adding Enteromorpha prolifera and microbial fermentation on growth, digestion and non-specific immune of juvenile sea cucumbers (Apostichopus japonicus Selenka).Main sources included Enteromorpha prolifera, Sargassum polycystum,Sargassum thunbergii, Ulva lactuca and so on.Five diets were formulated to contain 0, 10%, 20%, 30% and 40% of Enteromorpha prolifera, respectively.Half of each diet was fermented by the suspension of Brewer's yeast.The trial finally had 10 experimental diets.Each diet was randomly fed to one group with 3 replicates of 30 sea cucumber [average body weight of (3.94±0.46) g].The results showed as follows: 1) the weight gain rate (WGR), specific growth rate (SGR) and feed efficiency (FE) of sea cucumbers were increased with Enteromorpha prolifera adding ratio increasing among the non-fermented groups, and the group of 40% Enteromorpha prolifera was the best; the WGR, SGR and FE of sea cucumbers were firstly increased and then decreased with Enteromorpha prolifera adding ratio increasing among the fermented groups, and the group of 30% Enteromorpha prolifera was the best.The WGR, SGR and FE in the group of 30% and 40% Enteromorpha prolifera were not significantly different (P>0.05), but all of them were significantly higher than those in the group of 10% Enteromorpha prolifera (P<0.05).The growth performance indices of sea cucumbers were not significantly affected by microbial fermentation (P>0.05).2) The intestinal cellulase activity was increased in the first stage and then decreased with Enteromorpha prolifera adding ratio increasing among the non-fermented groups.However, the intestinal cellulase activity tended to increase with Enteromorpha prolifera adding ratio increasing among the fermented groups.In the same adding ratio, the intestinal cellulase activity in the fermented groups was higher than that in the non-fermented groups, and the significant differences were found when adding ratios were 10%, 20% and 40% (P<0.05).There was significant interaction between yes or no fermentation and Enteromorpha prolifera adding ratio on the intestinal cellulase activity (P<0.05), but no significant interactions on intestinal amylase and trypsin activities (P>0.05).3) The superoxide dismutase (SOD), acid phosphatase (ACP) and alkaline phosphatase (AKP) of coelomic fluid were not significantly affected by yes or no fermentation and Enteromorpha prolifera adding ratio (P>0.05), and the yes or no fermentation and Enteromorpha prolifera adding ratio had no significant interactions on them.It can be concluded that the optimal adding ratio of Enteromorpha prolifera in the diet of juvenile sea cucumber is between 30% and 40%.And microbial fermentation can promote the intestinal cellulase activity of juvenile sea cucumber to some extent.

Key words： Enteromorpha prolifera; sea cucumbers (Apostichopus japonicus Selenka); fermentation; growth; cellulase; non-specific immune

Cite this article

LI Meng, LIAO Meijie, WANG Yingeng, CHANG Qing, LI Bin, ZHANG Zhen, FAN Ruiyong . Effects of Enteromorpha prolifera Adding Ratio and Microbial Fermentation on Growth, Digestion and Non-Specific Immune of Juvenile Sea Cucumbers (Apostichopus japonicus Selenka)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(10) : 3270 -3278 . DOI: 10.3969/j.issn.1006-267x.2015.10.036

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