Nutritional Values of Elymus sibiricus Linn at Different Growing Stages: Determined by the Methods of in Vitro Gas Production and Cornell Net Carbohydrate and Protein System

SI Xuemeng, ZHAO Donghui, SHENG Yufei, ZHANG Zhengfan, GUO Chunhua, CHEN Guangji

doi:10.3969/j.issn.1006-267x.2015.10.039

2015 , Vol. 27 >Issue 10: 3293 - 3301

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.10.039

Short Communications

Nutritional Values of Elymus sibiricus Linn at Different Growing Stages: Determined by the Methods of in Vitro Gas Production and Cornell Net Carbohydrate and Protein System

Expand

College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610225, China

Received date: 2015-04-14

Online published: 2015-10-15

Fold

Abstract

This study was conducted to evaluate the nutritional values of Elymus sibiricus Linn at different growing stages by the methods of in vitro gas production and Cornell net carbohydrate and protein system (CNCPS).Gas production and fermentation parameters were determined by in vitro gas production method during heading, flowering, filling and milk ripe stages, respectively.The CNCPS component contents were determined, and the correlation between the two methods was analyzed.The results showed as follows: 1) with the growing of Elymus sibiricus Linn, crude protein (CP) and soluble protein (SOLP) contents decreased, neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) contents increased, and NDF insoluble protein (NDFIP) and ADF insoluble protein (ADFIP) contents decreased; the differences between heading and flowering stages compared with filling and milk ripe stages were significant (P<0.05).2) After 72 h in vitro fermentation, gas production of flowering stage was significantly greater than other stages (P<0.05).3) With the growing of Elymus sibiricus Linn, in carbohydrate (CHO) components, the contents of CHO and slowly degraded carbohydrate (CB₂) increased, while those of rapidly degraded carbohydrate (CA) and nonstructural carbohydrate (CNSC) showed an opposite tend; in protein components, unavailable protein (PC) content at heading stage and non-protein nitrogen (PA) content at flowering stage were significantly higher than the other stages (P<0.05).4) CNSC, CA, intermediately degraded carbohydrate (CB₁), CB₂, unavailable fiber (CC), PA, slowly degraded true protein (PB₃) and PC contents were correlated with the gas production at different time point (P<0.05 or P<0.01), and regression equations were set up.In conclusion, flowering stage of Elymus sibiricus Linn has the highest nutritional value, followed by the heading, filling, and milk ripe stages, and it is able to estimate the feed CNCPS component using in vitro gas production method.

Key words： Elymus sibiricus Linn; growing stage; CNCPS; in vitro gas production method; nutritional value; regression model

Cite this article

SI Xuemeng, ZHAO Donghui, SHENG Yufei, ZHANG Zhengfan, GUO Chunhua, CHEN Guangji . Nutritional Values of Elymus sibiricus Linn at Different Growing Stages: Determined by the Methods of in Vitro Gas Production and Cornell Net Carbohydrate and Protein System[J]. Chinese Journal of Animal Nutrition, 2015 , 27(10) : 3293 -3301 . DOI: 10.3969/j.issn.1006-267x.2015.10.039

References

[1] 陈默君,贾慎修.中国饲用植物[M].北京:中国农业出版社,2002.

[2] 李造哲,马青枝,云锦风,等.披碱草和野大麦及其杂种F1与BC1过氧化物酶同工酶分析[J].草业学报,2001,10(3):38-41.

[3] 王元富,杨智永,盘朝邦.川草2号老芒麦选育报告[J].四川草原,1995(1):19-24.

[4] 赵广永.用净碳水化合物-蛋白质体系评定反刍动物饲料营养价值[J].中国农业大学学报,1994,4(S):71-76.

[5] 洪金锁,刘书杰,柴沙驼,等.体外产气法与尼龙袋法评定青海当地燕麦青干草营养价值[J].中国畜牧兽医,2009,36(3):36-38.

[6] HUHTANEN P,SEPPÄLÄ A,OTS M,et al.In vitro gas production profiles to estimate extent and effective first-order rate of neutral detergent fiber digestion in the rumen[J].Journal of Animal Science,2008,86(3):651-659.

[7] 曲永利,吴健豪,李铁.应用康奈尔净碳水化合物-蛋白质体系评定东北农区奶牛饲料营养价值[J].动物营养学报,2010,22(1):201-206.

[8] 陶春卫,张爱忠,姜宁,等.用CNCPS评定反刍动物几种常用粗饲料营养价值的研究[J].草食家畜,2009(3):50-54.

[9] MENKE K H,STEINGASS H.Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research Development,1988,28(1):7-55.

[10] 崔占鸿.用体外产气法评价双低菜籽饼与藏嵩草的组合效应[J].家畜生态学报,2012,33(2):68-72.

[11] ØRSKOV E R,MCDONALD I.The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage[J].The Journal of Agricultural Science,1979,92(2):499-503.

[12] 张丽英.饲料分析及饲料质量检测技术[M].2版.北京:中国农业大学出版社,2003.

[13] VAN SOEST P J,SNIFFEN C J,MERTENS D R,et al.A net protein system for cattle:the rumen submodel for nitrogen[C]//OWENS F N.Protein requirements for cattle:proceedings of an international symposium.Stillwater:Oklahoma State University,1981:265.

[14] KRISHNAMOORTHY U,SNIFFEN C J,STERN M D,et al.Evaluation of a mathematical model of rumen digestion and an in vitro simulation of rumen proteolysis to estimate the rumen-undegraded nitrogen content of feedstuffs[J].British Journal of Nutrition,1983,50(3):555-568.

[15] AACC.Approved methods of the AACC[M].Saint Paul:American Association of Cereal Chemistry,1976.

[16] SNIFFEN C J,O'CONNOR J D,VAN SOEST P J,et al.A net carbohydrate and protein system for evaluating cattle diets:Ⅱ.carbohydrate and protein availability[J].Journal of Animal Science,1992,70(11):3562-3577.

[17] 李孟良,高志炜.播娘蒿不同生育期鲜草产量与营养价值[J].草业学报,2004,13(5):66-69.

[18] 马召朋,栗孟飞,杨德龙,等.不同水分条件下冬小麦灌浆期茎叶可溶性碳水化合物积累转运与籽粒灌浆的关系[J].草业学报,2014,23(4):68-78.

[19] 杨云贵,程天亮,杨雪娇,等.3个燕麦品种不同收获期对青贮饲草营养价值的影响[J].草地学报,2013,21(4):683-688.

[20] 宋中齐,干友民,田刚,等.多花黑麦草在生长肉兔上的营养价值评定[J].草业学报,2014,23(5):352-358.

[21] 刘春英,孙学映,朱体超,等.不同黑麦草品种生产性能比较与优势品种筛选[J].草业学报,2014,23(4):39-48.

[22] 汤少勋,姜海林,周传社,等.不同牧草品种对体外发酵产气特性的影响[J].草业学报,2005,14(3):72-77.

[23] 张桂杰,王红梅,罗海玲,等.应用体外产气与活体外消化法评定不同生育期禾本科牧草营养价值[J].营养饲料,2012,48(23):60-63,70.

[24] 张吉鹍,李龙瑞,邹庆华.江西几种奶牛常用饲料的多体系营养价值评定[J].江西农业大学学报,2012,34(5):1003-1007.

[25] AQUINO D L,TEDESCHI L O,LANZAS C,et al.Evaluation of CNCPS predictions of milk production of dairy cows fed alfalfa silage[C]//Proceedings of the Cornell Nutritional Conference.[S.l]:[s.n.]2003:137-150.

[26] 隋美霞.产气法和CNCPS法对粗饲料营养评价的比较研究[D].硕士学位论文.哈尔滨:东北农业大学,2009.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References