Selection of Reference Genes in Small Intestinal Mucosa of Hu Lambs

MA Zhiyuan; WENG Xiuxiu; LI Fei; LI Fadi; WANG Weimin; LIU Ting

doi:10.3969/j.issn.1006-267x.2015.11.019

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 11: 3478 - 3484

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.11.019

MOLECULAR NUTRITION

Selection of Reference Genes in Small Intestinal Mucosa of Hu Lambs

MA Zhiyuan ,
WENG Xiuxiu ,
LI Fei ,
LI Fadi ,
WANG Weimin ,
LIU Ting

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1. State Key Laboratory of Grassland Agro-Ecosystem, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
2. Gansu Province Mutton Sheep Breeding Biotechnology Engineering Laboratory, Minqin 733300, China;
3. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2015-06-17

Online published: 2015-11-21

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Abstract

Six Hu lambs were used and three of them were slaughtered at 42 and 84 days of age separately to select a suitable reference gene for real time-PCR (RT-PCR) of lamb intestinal mucosa. Stabilities of four candidate housekeeping genes glyceraldehyde phosphate dehydrogenase (GAPDH), phosphoglycerate kinase 1 (PGK1), 18S rRNA and β-actin (ACTB) were measured in mucosa of duodenum, jejunum and ileum. The results showed as follow:four candidate housekeeping genes' specific gene fragments were detected; the expression of PGK1 and ACTB turned out to be the most stable between mucosa of three small intestinal compartments; and all four candidate genes showed a constant expression between 42 and 84 days of age; the expression of ACTB is the most stable gene according the analysis of ΔCt method and geNorm software. It is concluded that ACTB is the best reference gene of RT-PCR for Hu lambs small intestinal mucosa.

Key words： real time-PCR; reference gene; Hu sheep; small intestinal mucosa

Cite this article

MA Zhiyuan , WENG Xiuxiu , LI Fei , LI Fadi , WANG Weimin , LIU Ting . Selection of Reference Genes in Small Intestinal Mucosa of Hu Lambs[J]. Chinese Journal of Animal Nutrition, 2015 , 27(11) : 3478 -3484 . DOI: 10.3969/j.issn.1006-267x.2015.11.019

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